Degradation of fluoranthene, pyrene, benz[ a ]anthracene and dibenz[ a , h ]anthracene by Burkholderia cepacia

Abstract

Microbiological analysis of soils from a polycyclic aromatic hydrocarbon (PAH)‐contaminated site resulted in the enrichment of five microbial communities capable of utilizing pyrene as a sole carbon and energy source. Communities 4 and 5 rapidly degraded a number of different PAH compounds. Three pure cultures were isolated from community 5 using a spray plate method with pyrene as the sole carbon source. The cultures were identified as strains of Burkholderia (Pseudomonas) cepacia on the basis of biochemical and growth tests. The pure cultures (VUN 10 001, VUN 10 002 and VUN 10 003) were capable of degrading fluorene, phenanthrene and pyrene (100 mg l−1) to undetectable levels within 7–10 d in standard serum bottle cultures. Pyrene degradation was observed at concentrations up to 1000 mg l−1. The three isolates were also able to degrade other PAHs including fluoranthene, benz[a]anthracene and dibenz[a,h]anthracene as sole carbon and energy sources. Stimulation of dibenz[a,h]anthracene and benzo[a]pyrene degradation was achieved by the addition of small quantities of phenanthrene to cultures containing these compounds. Substrate utilization tests revealed that these micro‐organisms could also grow on n‐alkanes, chlorinated‐ and nitro‐aromatic compounds.

Changes in bacterial community of anthracene bioremediation in municipal solid waste composting soil

“…Many different bacterial genera previously isolated from PAH-contaminated sites belong to Sphingomonas (Pinyakong et al, 2000), Paracoccus (Teng et al, 2010), Mycobacterium (Luo, 2008), Comamonas (Goyal and Zylstra, 1996), Pseudomonas (Jacques et al, 2005;Santos et al, 2008), Burkholderia (Juhasz et al, 1997), Janibacter (Zhang et al, 2009), and Sphingobium (Cunliffe and Kertesz, 2006). Enrichment of PAH-degraders is a common phenomenon during the course of PAH biodegradation (Guo et al, 1997;Piskonen et al, 2005).…”

Content of potentially bioavailable polycyclic aromatic hydrocarbons in rhizosphere soil in relation to properties of soils

“…5-rings). For example Juhasz et al (1997) showed a visible increase of dibenz [ah]anthracene and benzo [a]anthracene mineralization in the presence of fluoranthene. Some earlier studies (Oleszczuk and Baran, 2003b) showed also a limitation of the 5-rings PAH mineralization in the case of the quicker degradation of 3-and 4-rings compounds.…”

Toluene Degrading Bacteria from the Rhizosphere of Solanum melongena Contaminated with Polycyclic Aromatic Hydrocarbon

“…Also, microbes derive energy necessary to degrade contaminants from the catabolic degradation of contaminants themselves. Because of all these properties, microbes are used in situ to minimize disturbance of the pollutants from contaminated site (Juhasz et al, 1997 (Bisht et al, 2010). When a suitable rhizospheric strain is introduced together with a suitable plant, it settles on the root along with indigenous population, thereby enhancing the bioremediation process.…”