Catechin prevents the calcium oxalate monohydrate induced renal calcium crystallization in NRK-52E cells and the ethylene glycol induced renal stone formation in rat

Abstract

BackgroundReactive oxygen species play important roles in renal calcium crystallization. In this study, we examined the effects of catechin, which have been shown to have antioxidant properties on the renal calcium crystallization.MethodsIn the vitro experiment, the changes of the mitochondrial membrane potential, expression of superoxide dismutase (SOD), 4-hydroxynonenal (4-HNE), cytochrome c, and cleaved caspase 3 were measured to show the effects of catechin treatment on the NRK-52E cells induced by calcium oxalate monohydrate (COM). In the vivo study, Sprague–Dawley rats were administered 1% ethylene glycol (EG) to generate a rat kidney stone model and then treated with catechin (2.5 and 10 mg/kg/day) for 14 days. The urine and serum variables were dected on 7 and 14 days after EG administration. The expression of cytochrome c, cleaved caspase 3, SOD, osteopontin (OPN), malondialdehyde (MDA), 8-hydroxy-2′-deoxyguanosine (8-OHdG) in kidney were measured. Furthermore, the mitochondrial microstructure in the kidney was also examined by transmission electron microscopy.ResultsCatechin treatment could prevent the changes in mitochondrial membrane potential and expression of SOD, 4-HNE, cytochrome c, and cleaved caspase 3 in NRK-52E cells induced by the COM. For the in vivo experiments, the EG administration induced renal calcium crystallization was also prevented by the catechin. The expression of SOD, OPN, MDA, OPN and 8-OHdG, were increased after EG administration and this increase was diminished by catechin. Moreover, catechin also prevented EG induced mitochondrial collapse in rat.ConclusionsCatechin has preventive effects on renal calcium crystallization both in vivo and in vitro, and provide a potential therapeutic treatment for this disease.

Oral administration of indigenous oxalate degrading lactic acid bacteria and quercetin prevents calcium oxalate stone formation in rats fed with oxalate rich diet

“…In addition, Siener, Bade et al (2013) suggested that LAB preparation may be altered to select the highest oxalate degrading activity. Oxalate induced toxicity enhanced the oxidative stress resulting as renal epithelial injury and tissue damage which could be attenuated using the exogenous antioxidant supplements both in vitro and in vivo (Farooq et al, 2014;Zhai et al, 2013). Quercetin decreased the lipid peroxidation and inhibited CaOx crystal deposition in rats fed with ethylene glycol (Zhu et al, 2014).…”

Preventive Effect of Liupao Tea Polyphenols on HCl/Ethanol-Induced Gastric Injury in Mice

“…Gallic acid can inhibit oxidative stress and inflammation to improve gastric injury [31]. Catechins can also protect tissues by reducing the content of malondialdehyde and increasing the activity of superoxide dismutase [32]. Epicatechin also has the effect of alleviating the disease through its antioxidation function.…”

Microvillar injury in renal tubular epithelial cells induced by calcium oxalate crystal and the protective role of epigallocatechin‐3‐gallate

“…Moreover, the induction of oxidative stress may be responsible for apical microvillar breakdown by reorganization of the actin cytoskeleton (40, 41). Catechin, which is one of the main components of green tea extract, acts as a free radical scavenger and can prevent COM crystallization and renal stone formation in rats (42, 43). EGCG has been identified as a chemoprotective agent with an ability to inhibit proteasome function (44).…”