Catechin as the Most Efficient Bioactive Compound from Azadirachta indica with Antibiofilm and Anti-quorum Sensing Activities Against Dental Biofilm: an In Vitro and In Silico Study

Lahiri, Dibyajit; Nag, Moupriya; Dutta, Bandita; Mukherjee, Indranil; Ghosh, Sutanu; Dey, Ankita; Banerjee, Ritwik; Ray, Rina Rani

doi:10.1007/s12010-021-03511-1

Cited by 46 publications

(25 citation statements)

References 29 publications

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“…In this research, it was possible to observe an inhibition of total growth from the first incubation time (30 min) using SeNPs ( Figure 8 a,b). It could be due to the content of bioactive compounds in the Cof-Met extracts present on the surface of the SeNPs, capable of damaging the signaling receptors necessary to carry out quorum sensing (QS) [ 50 ], a chemical process of cell-to-cell communication used by bacteria such as A. faecalis to regulate their virulence and the formation of biofilms [ 51 , 52 ]. This QS inhibition has been reported using SeNPs biosynthesized with AsAc, with the average size of 85 nm, where the inhibition of the biosynthesis of crucial QS compounds such as violacein (80%) was demonstrated, suggesting the disruption of the QS signal [ 53 ].…”

Section: Resultsmentioning

confidence: 99%

Antibacterial Activity of Biosynthesized Selenium Nanoparticles Using Extracts of Calendula officinalis against Potentially Clinical Bacterial Strains

et al. 2021

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The use of selenium nanoparticles (SeNPs) in the biomedical area has been increasing as an alternative to the growing bacterial resistance to antibiotics. In this research, SeNPs were synthesized by green synthesis using ascorbic acid (AsAc) as a reducing agent and methanolic extract of Calendula officinalis L. flowers as a stabilizer. Characterization of SeNPs was performed by UV-vis spectrophotometry, infrared spectrophotometry (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM) techniques. SeNPs of 40–60 nm and spherical morphologies were obtained. The antibacterial activity of marigold extracts and fractions was evaluated by disk diffusion methodology. The evaluation of SeNPs at different incubation times was performed through the colony-forming unit (CFU) count, in both cases against Serratia marcescens, Enterobacter cloacae, and Alcaligenes faecalis bacteria. Partial antibacterial activity was observed with methanolic extracts of marigold leaves and flowers and total inhibition with SeNPs from 2 h for S. marcescens, 1 h for E. cloacae, and 30 min for A. faecalis. In addition, SeNPs were found to exhibit antioxidant activity. The results indicate that SeNPs present a potentiated effect of both antimicrobial and antioxidant activity compared to the individual use of marigold extracts or sodium selenite (Na2SeO3). Their application emerges as an alternative for the control of clinical pathogens.

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Section: Resultsmentioning

confidence: 99%

Antibacterial Activity of Biosynthesized Selenium Nanoparticles Using Extracts of Calendula officinalis against Potentially Clinical Bacterial Strains

et al. 2021

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“…It was determined that catechin (flavan‐3‐ol) isolated from Azadirachta indica has strong antibiofilm effect on the dental plaque caused by P. gingivalis and Alcaligenes faecalis in vitro , catechin was further docked with quorum sensing LuxS protein (PDB ID: 6SLI) which also showed considerable interaction (Lahiri et al . 2021). In another study, both of in vitro and in silico studies were done to prove the anti‐quorum sensing potential of Senegalia nigrescens by selected phytochemicals and suggested that the S. nigrescens extracts and phytochemicals such as melanoxetin and quercetin‐3‐ O ‐methyl ether, have ability to reduce virulence and pathogenicity of drug‐resistant bacteria in vivo (Bodede et al .…”

Section: Resultsmentioning

confidence: 99%

Phytochemical profile, antimicrobial, and anti‐quorum sensing properties of fruit stalks ofPrunus aviumL.

Önem

Sarisu

Özaydın

et al. 2021

Lett Appl Microbiol

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The aim of this study is to investigate the phytochemical contents and antibacterial properties of 2‐year Prunus avium L. standard cultivars [Cristalina (Cr), 0900 Ziraat (Zr)] and to elucidate the mechanism of action of the extracts on the quorum sensing (QS) system by using homology modelling and molecular docking. Phenolic contents of methanol extract of Cr and Zr stalks were detected by HPLC. As a result, catechin hydrate (6364·67–8127·93 µg g−1) and chlorogenic acid (998·81–1273·4 µg g−1) were found to be the highest in stalk extracts in the two varieties in 2017. All extracts had inhibitory effect on Gram‐positive bacteria. Stalk extract of Zr showed higher inhibition rate (86%) on swarming motility. Stalk samples of Zr collected in 2017 and 2018 also reduced biofilm formation by 75 and 73%, respectively. The computational analysis revealed that one of the major component of the extracts, chlorogenic acid, was able to bind to the QS system receptors, LasR, RhlR, and PqsR. Therefore, the mechanism of decreasing the production of virulence factors by the extracts might be through inhibiting these receptors and thus interfering with the QS system.

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“…Neem contains highly water-soluble phytochemicals (flavonoids, tannins, polyphenols, catechins, gallic acid, quercetins, and saponins) with more health-promoting and biological effects [ 82 ]. It is anticipated that neem leaf extract targeted at sites other than those used by antibiotics will be effective against drug-resistant pathogens [ 83 ].…”

Section: Discussionmentioning

confidence: 99%

Ameliorative Effect of Neem Leaf and Pomegranate Peel Extracts in Coccidial Infections in New Zealand and V-Line Rabbits: Performance, Intestinal Health, Oocyst Shedding, Carcass Traits, and Effect on Economic Measures

Mohammed

Sallam

Basuni

et al. 2021

Animals

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Healthy, weaned, coccidial-free male rabbits from two breeds (New Zealand white (NZ) and V-line (VL)) were divided into 10 equal groups (5 groups each for NZ and VL) (3 replicates/group, 6 rabbits/replicate, 18 rabbits/group). All rabbits were inoculated with 5 × 104 Eimeria spp. oocysts (E. intestinalis (67%), E. magna (22%), and E. media (11%)) except for the rabbits in the first group (G1), which were inoculated with a sterile solution and served as a negative control. The remaining four groups were treated as follows: G2, no treatment/positive control, G3, treated with neem leaf extract, G4, treated with pomegranate peel extract (PPE), and G5, treated with a combination of neem leaf extract and PPE. For both breeds, our results showed that the use of neem leaf and/or pomegranate peel extract resulted in improved growth performance, with a significant improvement in relative feed conversion ratio (FCR) compared to the positive control groups, which recorded the worst values, as well as a significant (p ≤ 0.05) reduction in mean oocyst count compared to the positive control groups. We also observed downregulation of mRNA levels of IL-1βα, IL6, and TNF-α in the herbal treatment groups compared with the mRNA levels of these genes in the positive control groups. Herbal treatment with neem leaf and/or pomegranate peel extracts had positive effects on the NZ and VL rabbits experimentally infected with mixed Eimeria species, as evidenced by their healthy appearance, good appetite, no mortalities, an anticoccidial index > 120, and a significantly higher total return and net profit when compared to the positive control groups of both breeds. In NZ rabbits, the treatment with neem leaf extract alone (G3) or in combination with PPE (G5) recorded the most efficient economic anticoccidial activity.

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Catechin as the Most Efficient Bioactive Compound from Azadirachta indica with Antibiofilm and Anti-quorum Sensing Activities Against Dental Biofilm: an In Vitro and In Silico Study

Cited by 46 publications

References 29 publications

Antibacterial Activity of Biosynthesized Selenium Nanoparticles Using Extracts of Calendula officinalis against Potentially Clinical Bacterial Strains

Antibacterial Activity of Biosynthesized Selenium Nanoparticles Using Extracts of Calendula officinalis against Potentially Clinical Bacterial Strains

Phytochemical profile, antimicrobial, and anti‐quorum sensing properties of fruit stalks ofPrunus aviumL.

Ameliorative Effect of Neem Leaf and Pomegranate Peel Extracts in Coccidial Infections in New Zealand and V-Line Rabbits: Performance, Intestinal Health, Oocyst Shedding, Carcass Traits, and Effect on Economic Measures

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