(+)-Catechin and (-)-epigallocatechin gallate: are these promising antioxidant therapies for frozen goat semen?

Silva, Ellen Cordeiro Bento da; Arruda, Lúcia Cristina Pereira; Vieira, Joane Isis Travassos; Soares, Pierre Castro; Guerra, Maria Madalena Pessoa

doi:10.1590/1678-4162-10539

Cited by 10 publications

(13 citation statements)

References 24 publications

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“…The antioxidant activity of EGCG is polyphenol groups of catechin as chelating agents, whereas flavonoids could favor divesting its catalytic activity to the lipid bilayers membranes [ 20 ]. Epigallocatechin-3-gallate (EGCG) is considered the most promising bioactive compound in green tea due to its potent antioxidant activity [ 21 ]. EGCG interacts with the estrogen receptor (ER) or other proteins and/or lipids receptors in the plasma membrane, acted on signaling pathways to induced cyclic adenosine monophosphate, adenosine monophosphate-activated protein kinase, calcium ion, and ferric iron.…”

Section: Discussionmentioning

confidence: 99%

Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients

et al. 2021

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Objective: The aim of this study was to ascertain the effects of adding green tea extract (GTE) to skim milk-egg yolk (SM-EY) extender on both the quality of post-thawed bull semen and the pregnancy rates of the recipient cows.Methods: Twelve ejaculates from four Simmental bulls, aged 3 to 5 years and weighing 900 to 950 kg, were diluted SM-EY extender, added with 0, 0.05, 0.1, and 0.15 mg GTE/100 mL extender and then frozen. After four weeks storage in liquid nitrogen, the sperm were thawed and evaluated for viability, motility, intact plasma membrane (IPM), and DNA fragmentation. Meanwhile, the estrus cycles of 48 recipient cows were synchronized by intramuscular administration of a single injection of 5 mg prostaglandin F2α. Estrus cows were divided into four equal groups and inseminated artificially 18 to 20 h after the onset of estrus by using semen from each extender group. Pregnancy was diagnosed by measuring serum progesterone levels at 21 days, followed by transrectal palpation 90 days after insemination.Results: The findings revealed that adding 0.1 mg of GTE/100 mL extender produced the highest percentages of sperm viability (70.67%±1.75%), motility (69.17%±1.47%), and IPM (69.23%±1.21%) and the lowest percentage of DNA fragmentation (3.00%±0.50%). The pregnancy diagnosis revealed that all cows (36/36) inseminated using frozen semen in GTE addition extender were pregnant (pregnancy rate 100%), whereas the pregnancy rate of the control group was 83.33% (10/12).Conclusion: It may be concluded that 0.1 mg GTE/100 mL extender yields the best quality of spermatozoa and that all variants doses of GTE in extender produce a higher pregnancy rate among recipient cows.

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Section: Discussionmentioning

confidence: 99%

Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients

et al. 2021

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“…Contradictory observations are, however, available with respect to the impact of EPI on male fertility. A number of in vivo [14,15], as well as in vitro studies [16,17] emphasize the ability of EPI to exhibit protective and antioxidant effects on male reproductive cells and tissues. Furthermore, EPI has been shown to reverse the reprotoxic effects caused by a variety of endogenous [15] or exogenous risk factors [18,19].…”

Section: Introductionmentioning

confidence: 99%

Epicatechin Provides Antioxidant Protection to Bovine Spermatozoa Subjected to Induced Oxidative Stress

Tvrdá

Straka

Galbavy³

et al. 2019

Molecules

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Epicatechin (EPI) is a natural flavonoid with antibacterial, anti-inflammatory and anti-cancer properties. Furthermore, the molecule exhibits powerful reactive oxygen species (ROS) scavenging and metal-chelating properties. In this study, we assessed the efficiency of EPI to reverse ROS-mediated alterations to the motility, viability, DNA integrity and oxidative profile of bovine spermatozoa. For the first experiment, spermatozoa were washed out of fresh semen and exposed to 12.5 μmol/L EPI, 25 μmol/L EPI, 50 μmol/L EPI and 100 μmol/L EPI in the presence of ferrous ascorbate (FeAA) during a 6 h in vitro culture. For the second experiment, the ejaculates were split into aliquots and cryopreserved with a commercial semen extender supplemented with 12.5 μmol/L EPI, 25 μmol/L EPI, 50 μmol/L EPI, 100 μmol/L EPI or containing no supplement. Sperm motility was assessed using the computer-aided sperm analysis and the cell viability was studied with the metabolic activity test. ROS production was quantified using luminometry, and DNA fragmentation was evaluated using the chromatin dispersion test. Cell lysates were prepared at the end of the culture in order to assess the concentration of protein carbonyls and malondialdehyde. Exposure to FeAA led to a significantly reduced sperm motility (p < 0.001), mitochondrial activity (p < 0.001), but increased the generation of ROS (p < 0.001), as well as oxidative damage to proteins (p < 0.001), DNA (p < 0.001) and lipids (p < 0.001). EPI supplementation, particularly at a concentration range of 50–100 μmol/L, resulted in higher preservation of the spermatozoa vitality (p < 0.001). Furthermore, 50–100 μmol/L EPI were significantly effective in the prevention of oxidative damage to sperm proteins (p < 0.001), lipids (p < 0.001) and DNA (p < 0.01 in relation to 50 μmol/L EPI; p < 0.001 with respect to 100 μmol/L EPI). In the case of the cryopreserved spermatozoa, the administration of 50–100 μmol/L EPI resulted in higher sperm motility (p < 0.001) and mitochondrial activity (p < 0.001). ROS production, the number of protein carbonyls, lipid peroxidation as well as oxidative DNA damage were found to be significantly decreased particularly in samples cryopreserved in the presence of 100 μmol/L EPI (p < 0.001). Our results suggest that EPI could behave as an effective antioxidant which may prevent oxidative insults to spermatozoa, and thus, preserve their vitality and functionality. Nevertheless, its potential to achieve higher fertilization rates in reproductive technologies needs to be validated.

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“…For cryopreservation, was used the protocol described by Silva et al (2019), where the goat semen pools were diluted (1:9) in Tris buffer solution and centrifuged twice (3000 rpm; 10 min). Then, the spermatozoa pellets were diluted in skimmedmilk based extender, to a concentration of 200x10 6 sperm/mL, fractionated and treated, according to the experimental groups (G1=control, G2=20 µM Trolox and G3=40 µM Trolox).…”

Section: Methodsmentioning

confidence: 99%

“…Regarding the study of plasma membrane integrity (PMi), parameter positively correlated with fertility (Lee et al, 2009) and most affected by semen cryopreservation (Castro et al, 2016), it was performed by double staining with carboxifluorescein diacetate (CFD) and propidium iodide (PI) (Silva et al, 2019). A total of 200 spermatozoa were evaluated under an epifluorescence microscope (Carl Zeiss, Germany; 400x) and classified as carriers of intact membrane, when stained in green, or damaged membrane, when stained in red.…”

Section: Methodsmentioning

confidence: 99%

“…The acrosome integrity, essential to the fertility (Fernandes & Pimentel, 1997), was evaluated through fluoresceine isothiocyanate-conjugated peanut agglutinin (FITC-PNA) (Silva et al, 2019). A total of 200 spermatozoa per slide were analyzed under a fluorescence microscope (Carl Zeiss, Germany) and classified as having intact acrosome (Aci), when stained in green, or reacted acrosome (Acr), when stained in mixed green, without staining or stained only in the equatorial region of the sperm head.…”

Section: Methodsmentioning

confidence: 99%

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Effect of trolox added to freezing extenders over goat and ram spermatozoa

Arruda

Silva

Batista

et al. 2021

RSD

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Semen cryopreservation is responsible for decrease the gamete fertility, due to structural and functional damages. Among the various causes, oxidative stress, resulting from the higher generation of reactive oxygen species (ROS), has been attributed to affect semen quality. Thus, it was objectified evaluate the effect of Trolox on ram and goat sperm, subjected to freezing. Semen pools of goat (n=5) and ram (n=6) were diluted in skimmed milk (7% glycerol) or Tris-egg yolk (5% glycerol) extender, respectively, added or not of Trolox (0, 20 or 40 μM/ml) and frozen. After thawing (37 °C/30 s), aliquots of semen were evaluated for lipid peroxidation by high performance liquid chromatography, coupled with a photodiode array detector (HPLC-DAD), and flow cytometry (C11-BODIPY581/591), besides of plasma membrane and acrosome integrity by fluorescence microscopy, and sperm kinetics by computerized sperm analysis (CASA). The antioxidant treatment with Trolox did not determine significant effects (p>0.05) on lipid peroxidation, plasma membrane integrity, acrosomal integrity and on the kinetic parameters evaluated. Thus, it is concluded that Trolox (20 or 40 μM) did not have a protective or deleterious effect on goats and ram sperm, submitted to freezing.

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(+)-Catechin and (-)-epigallocatechin gallate: are these promising antioxidant therapies for frozen goat semen?

Cited by 10 publications

References 24 publications

Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients

Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients

Epicatechin Provides Antioxidant Protection to Bovine Spermatozoa Subjected to Induced Oxidative Stress

Effect of trolox added to freezing extenders over goat and ram spermatozoa

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