Catalytically Active Nanomaterials: Artificial Enzymes of Next Generation

Singh, Sanjay

doi:10.15226/2374-8141/5/1/00150

Cited by 9 publications

(12 citation statements)

References 41 publications

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“…The development of nanostructures that mimic the remarkable functions and catalytic properties of natural enzymes, i.e., to create nanozymes [1,2], has been an exciting topic in the field of nanotechnology for the last two decades [3]. Among several approaches used to create nanozymes [4,5], the self-assembly of thiolated ligands onto the surface of gold nanoparticles (Au-NPs) to obtain monolayer-protected gold clusters (Au-MPCs) has emerged as a powerful strategy [6].…”

Section: Introductionmentioning

confidence: 99%

Peptide–Gold Nanoparticle Conjugates as Artificial Carbonic Anhydrase Mimics

Mikolajczak

Koksch

2019

Catalysts

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We herein describe the design and synthesis of a catalytically active peptide–gold nanoparticle conjugate (Pep-Au-NP) that binds Zn(II) within its peptide monolayer and develops carbonic anhydrase activity. Specifically, a modified variant of the β-sheet forming IHIHIQI-peptide (IHQ), which forms an interstrand 3-His Zn(II)-binding site, was used as a ligand for spherical gold nanoparticles (Au-NPs). The resulting immobilized peptide maintains its ability to form β-sheets, as determined by circular dichroism (CD)-spectroscopy and, thus, maintains its ability to form Zn(II)-binding sites. The addition of Zn(II)-ions to the peptide–gold nanoparticle conjugates (Au@IHQ-NP) resulted in significant improvements in rates of ester hydrolysis of 4-nitrophenyl acetate (4-NPA) and the hydration of CO2 compared to the unconjugated peptide variants. Recycling of the catalyst revealed that Au@IHQ-NP remains intact with at least 94% of its initial activity after five rounds of CO2 hydration. The herein reported results reveal that Pep-Au-NPs are able to perform reactions catalyzed by natural metalloenzymes and open up new possibilities for the implementation of these conjugates.

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Section: Introductionmentioning

confidence: 99%

Peptide–Gold Nanoparticle Conjugates as Artificial Carbonic Anhydrase Mimics

Mikolajczak

Koksch

2019

Catalysts

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“…TMB (3,3,5,5-tetramethylbenzidine), DOPA (dihydroxyphenylalanine) (adopted from Singh, 2017) One of the first superoxide dismutase activities determined was for cerium dioxide nanoparticles (Korsvik et al, 2007;Singh, 2016). In the case of superoxide anion dismutration, the formation of hydrogen peroxide and transitional compound -cerium hydroperoxide Се(ООН)(ОН) 3 on the surface of the nanodispersed cerium dioxide occurs, ie, three reactions actually occur (Batinić-Haberle et al, 2010):…”

Section: Fig 3 Enzyme-like Properties Of Nanomaterialsmentioning

confidence: 99%

Enzyme-like activity of nanomaterials

Tsekhmistrenko

Bityutskyy

Цехмістренко

et al. 2018

Regul. Mech. Biosyst.

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In modern conditions, nanomaterials, especially nanoparticles of metals and nonmetals, are increasingly used in various industries. Due to their unique properties, in particular, the ability of nanoparticles to exhibit an enzyme-like effect they are widely used in biology, medicine, biotechnology, the food industry and agriculture. Important advantages of nanoparticles are their size, which enables specific properties to be present: their large surface area, the ability to transfer molecules and the ability to protect them from degradation and release over a long time, the location of action and the specificity of interaction with biological structures. Nanoparticles play a special role in the processes of neutralizing the active forms of oxygen. It has been established that a number of nanoparticles, in particular, Fe, Mn, Zn, Ce, Si and Se oxides, have an enzyme-like activity mimicking that of some enzymes. By changing the degree of oxidation, these particles can regenerate and continuously catalyze the reaction of neutralizing superoxide anion radicals, thus fulfilling the function of SOD and being the first link in protecting tissues and cells from oxidative stress in physiological and pathological conditions. It is proved that nanoparticles Mn3O4, Fe3O4, Co3O4, CeO2, LaCoO3 and other elements can effectively dispose of hydrogen peroxide and other peroxides, showing catalase-like and peroxidase-like activity. Nanozymes are characterized that exhibit the activity of oxidases, peroxidases and phosphatase. The prospect of using mimetics for complex in vitro analyzes of high-sensitivity biomarker disease detection is shown. The possibility of effective multi-use of nanoparticles as antioxidants is indicated. There are good prospects for further research on properties and the use of polyfunctional particles that are easily synthesized, reliable and inexpensive. More work is needed to determine the interaction of enzymomimetics with biological molecules such as proteins, carbohydrates and lipids, and also to take into account the peculiarities of their metabolism, clearance, degradation, biocompatibility and side effects, since individual nanoparticles have the potential to be deposited in separate organs.

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“…There are multiple excellent reviews already available for each of our discussed emerging topics: nanozymes, [14,30] nano-/micromotors, [2,31] and microfluidics. [32][33][34][35] By cultivating interdisciplinarity as a habit of mind, our review discusses integration or interconnectedness of these emerging topics by focusing on i) generation of new ideas, ii) discussions across disciplinary boundaries (overlap of emerging topics allows looking at the problem from a variety of viewpoints-the same questions can be asked in different ways), and iii) solutions to complex problems, which are beyond the scope of a single discipline.…”

Section: Introductionmentioning

confidence: 99%

Micro‐Bio‐Chemo‐Mechanical‐Systems: Micromotors, Microfluidics, and Nanozymes for Biomedical Applications

et al. 2021

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Wireless nano‐/micromotors powered by chemical reactions and/or external fields generate motive forces, perform tasks, and significantly extend short‐range dynamic responses of passive biomedical microcarriers. However, before micromotors can be translated into clinical use, several major problems, including the biocompatibility of materials, the toxicity of chemical fuels, and deep tissue imaging methods, must be solved. Nanomaterials with enzyme‐like characteristics (e.g., catalase, oxidase, peroxidase, superoxide dismutase), that is, nanozymes, can significantly expand the scope of micromotors’ chemical fuels. A convergence of nanozymes, micromotors, and microfluidics can lead to a paradigm shift in the fabrication of multifunctional micromotors in reasonable quantities, encapsulation of desired subsystems, and engineering of FDA‐approved core–shell structures with tuneable biological, physical, chemical, and mechanical properties. Microfluidic methods are used to prepare stable bubbles/microbubbles and capsules integrating ultrasound, optoacoustic, fluorescent, and magnetic resonance imaging modalities. The aim here is to discuss an interdisciplinary approach of three independent emerging topics: micromotors, nanozymes, and microfluidics to creatively: 1) embrace new ideas, 2) think across boundaries, and 3) solve problems whose solutions are beyond the scope of a single discipline toward the development of micro‐bio‐chemo‐mechanical‐systems for diverse bioapplications.

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Catalytically Active Nanomaterials: Artificial Enzymes of Next Generation

Cited by 9 publications

References 41 publications

Peptide–Gold Nanoparticle Conjugates as Artificial Carbonic Anhydrase Mimics

Peptide–Gold Nanoparticle Conjugates as Artificial Carbonic Anhydrase Mimics

Enzyme-like activity of nanomaterials

Micro‐Bio‐Chemo‐Mechanical‐Systems: Micromotors, Microfluidics, and Nanozymes for Biomedical Applications

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