Catalytic properties of lipase from <i>Ficus trichopoda</i> and <i>Euphorbia unispina</i> latex: Study of their typoselectivity

Abstract

Objective: The search for lipase with distinct features, from plant latex is of great importance for industrial applications. The catalytic properties of lipases from Ficus trichopoda and Euphorbia unispina latex were characterized. Methodology and Results: Fresh latex from Ficus trichopoda and Euphorbia unispina were collected and dried through solar. Dried latex was taken for complete proximate analysis and their activity was analysed by thin layer chromatography. The two lipases were optimally active at pH=5 and temperature of 35°C and 50°C for Ficus trichopoda and Euphorbia unispina latex, respectively. The presence of metal ions enhances the activity of Ficus trichopoda latex, while no significant enhancement was observed in the case of Euphorbia unispina latex. Both lipases were able to hydrolyze saturated esters, and showed typo-selectivity for this group. However, the lipases are weak selective for the hydrolysis of unsaturated esters, especially for 18:2 fatty acids. Conclusions and application of finding: The enzyme from Ficus trichopoda latex was able to attack specific oil to generate free fatty acids or ester as the major product. This understanding may help in devising efficient methods to produce valuable modified oils.

Euphorbia species latex: A comprehensive review on phytochemistry and biological activities

“…Screening of Euphorbia latex has revealed the presence of many enzymes, including proteolytic enzymes that may be involved in plant defense against certain pathogens and external environmental conditions ( Domsalla et al, 2010 ; Fais et al, 2021 ). The catalytic properties of lipases contained in the latex of E. unispina have been described by Mazou et al (2017) . The optimum temperature and pH for the hydrolytic activity of the lipases were 50°C and 5, respectively.…”

Insights in the biocatalyzed hydrolysis, esterification and transesterification of waste cooking oil with a vegetable lipase

“…In this emerging topic of investigation, lipases from plant latex are also being studied, consisting CPL the standard of this kind of enzymes [42][43][44]. Other latex producing plant species having lipase activity belong to the Vascocellea (a close relative of the Caricaeae family) [30], Moraceae [45], Euphorbiaceae [46], and Apocynaceae [31,47], all showing very similar catalytic characteristics to CPL. Among the Apocynaceae family, the South American native climbing milkweed Araujia sericifera demonstrated lipase activity in its latex, whereupon which has been proved to be a promising biocatalyst for its use in the biorefinery industry [32,48].…”

Extraction and Purification of Lipases Enzyme from Germinating Seeds of Four Crops

“…Lipases can be extracted from microorganisms, including bacteria [9] and fungi [10], and from animals [11] and plants [12,13]. Plant lipases are extracted from different parts of the plant, such as fruits, latex and seeds.…”