Catalytic applications of enzymes encapsulated in metal–organic frameworks

Drout, Riki J.; Robison, Lee; Farha, Omar K.

doi:10.1016/j.ccr.2018.11.009

Cited by 227 publications

(167 citation statements)

References 159 publications

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“…Many biomacromolecules@ZIF-8, including enzymes@-ZIF-8, were recently developed as robust platform for biocatalysis,b iosensing, biobanking and bioprocessing. [10,11,56,57] Furthermore,i ncreasing efforts were devoted to construct biomacromolecule@nanoZIF-8 intracellular delivery systems for protein-and gene-based theranostics. [25,[58][59][60] These applications receive more and more attention because of the high loading efficiency and protecting effect of ZIF-8, and the convenient de novo synthesis process.H erein, we highlighted the importance of the embedding patterns for facilitating and preserving the biofunctionality of abiomacromolecule encapsulated within ZIF-8.…”

Section: Resultsmentioning

confidence: 99%

Modulating the Biofunctionality of Metal–Organic‐Framework‐Encapsulated Enzymes through Controllable Embedding Patterns

et al. 2020

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Embedding an enzyme within aMOF as exoskeleton (enzyme@MOF) offers new opportunities to improve the inherent fragile nature of the enzyme,but also to impart novel biofunctionality to the MOF.D espite the remarkable stability achieved for MOF-embedded enzymes,e mbedding patterns and conversion of the enzymatic biofunctionality after entrapment by aM OF have only received limited attention. Herein, we reveal howe mbedding patterns affect the bioactivity of an enzyme encapsulated in ZIF-8. The enzyme@MOF can maintain high activity when the encapsulation process is driven by rapid enzyme-triggered nucleation of ZIF-8. When the encapsulation is driven by slow coprecipitation and the enzymes are not involved in the nucleation of ZIF-8, enzy-me@MOF tends to be inactive owing to unfolding and competing coordination caused by the ligand, 2-methyl imidazole.T hese two embedding patterns can easily be controlled by chemical modification of the amino acids of the enzymes,modulating their biofunctionality.Supportinginformation and the ORCID identification number(s) for the author(s) of this article can be found under: https://doi.

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Section: Resultsmentioning

confidence: 99%

Modulating the Biofunctionality of Metal–Organic‐Framework‐Encapsulated Enzymes through Controllable Embedding Patterns

et al. 2020

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“…Many biomacromolecules@ZIF‐8, including enzymes@ZIF‐8, were recently developed as robust platform for biocatalysis, biosensing, biobanking and bioprocessing . Furthermore, increasing efforts were devoted to construct biomacromolecule@nanoZIF‐8 intracellular delivery systems for protein‐ and gene‐based theranostics .…”

Section: Resultsmentioning

confidence: 99%

Modulating the Biofunctionality of Metal–Organic‐Framework‐Encapsulated Enzymes through Controllable Embedding Patterns

et al. 2020

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Embedding an enzyme within a MOF as exoskeleton (enzyme@MOF) offers new opportunities to improve the inherent fragile nature of the enzyme, but also to impart novel biofunctionality to the MOF. Despite the remarkable stability achieved for MOF‐embedded enzymes, embedding patterns and conversion of the enzymatic biofunctionality after entrapment by a MOF have only received limited attention. Herein, we reveal how embedding patterns affect the bioactivity of an enzyme encapsulated in ZIF‐8. The enzyme@MOF can maintain high activity when the encapsulation process is driven by rapid enzyme‐triggered nucleation of ZIF‐8. When the encapsulation is driven by slow coprecipitation and the enzymes are not involved in the nucleation of ZIF‐8, enzyme@MOF tends to be inactive owing to unfolding and competing coordination caused by the ligand, 2‐methyl imidazole. These two embedding patterns can easily be controlled by chemical modification of the amino acids of the enzymes, modulating their biofunctionality.

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“…5,6 A variety of different strategies are amenable for incorporating biomimetic metal active sites into MOFs. These approaches include metallolinkers, 7 non-covalent encapsulation of molecular compounds and enzymes, 8,9 templated metal/ligand assemblies, 10 and the use of the metal ions or clusters comprising the secondary building units (SBUs). This perspective describes recent developments and future directions in bioinorganic chemistry and biomimetic catalysis centered at metal-organic framework SBUs.…”

Section: Biomimetic Chemistries In Crystalline Porous Materialsmentioning

confidence: 99%