Catalytic and thermodynamic properties of β-glucosidases produced by<i>Lichtheimia corymbifera</i>and<i>Byssochlamys spectabilis</i>

Morais, Tobias Pereira de; Barbosa, Paula Mirella Gomes; Garcia, Nayara Fernanda Lisbôa; Rosa-Garzon, Nathália Gonsales da; Fonseca, Gustavo Graciano; Paz, Marcelo Fossa da; Cabral, Hamilton; Leite, Rodrigo Simões Ribeiro

doi:10.1080/10826068.2018.1509083

Cited by 13 publications

(12 citation statements)

References 37 publications

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“…According to El-Shishtawy et al (2014), cultivations in wheat bran promote higher enzymatic production owing to their nutritional value, large surface area, better air circulation, and efficient penetration of mycelium from filamentous fungi. Previous work confirms wheat bran as an excellent substrate for β-glucosidase production by filamentous fungi (Santos et al 2016, Garcia et al 2018, Morais et al 2018.…”

Section: Resultsmentioning

confidence: 73%

“…The optimized parameter in each step was adopted in subsequent cultivation. All assays were performed in triplicate, and the described values represent the respective averages (Morais et al 2018).…”

Section: β-Glucosidase Production By Solid State Cultivationmentioning

confidence: 99%

“…Our research group has been working on the description of new biocatalysts from enzymeproducing filamentous fungi isolated from the Midwest region of Brazil, with appreciable biotechnological characteristics such as low production cost, high structural stability, and broad industrial applicability (Cavalheiro et al 2017, Costa et al 2019, Garcia et al 2015, Morais et al 2018). Among the strains recently isolated by our team, the thermophilic filamentous fungus Thermoascus crustaceus stood out for the production of the enzyme β-glucosidase.…”

Section: Introductionmentioning

confidence: 99%

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β-glucosidase from thermophilic fungus Thermoascus crustaceus: production and industrial potential

Garbin

Garcia

Cavalheiro

et al. 2021

An. Acad. Bras. Ciênc.

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Microbial β-glucosidases can be used in several industrial processes, including production of biofuels, functional foods, juices, and beverages. In the present work, production of β-glucosidase by solid state cultivation of the fungus Thermoascus crustaceus in a low-cost cultivation medium (comprising agroindustrial residues) was evaluated. The highest production of β-glucosidase, about 415.1 U/g substrate (or 41.51 U/mL), was obtained by cultivating the fungus in wheat bran with 70% humidity, during 96 h at 40°C. The enzymatic activity was optimum at pH 4.5 and 65°C. β-Glucosidase maintained its catalytic activity when incubated at a pH range of 4.0-8.0 and temperature of 30-55°C. The enzyme was strongly inhibited by glucose; even when the substrate and glucose concentrations were equal, the inhibition was not reversed, suggesting a noncompetitive inhibition. In the presence of up to 10% ethanol, β-glucosidase maintained its catalytic activity. In addition to β-glucosidase, the enzymatic extract showed activity of 36 U/g for endoglucanase, 256.2 U/g for xylanase, and 18.2 U/g for β-xylosidase. The results allow to conclude that the fungus T. crustaceus has considerable potential for production of β-glucosidase and xylanase when cultivated in agroindustrial residues, thereby reducing the cost of these biocatalysts.

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Section: Resultsmentioning

confidence: 73%

Section: β-Glucosidase Production By Solid State Cultivationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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β-glucosidase from thermophilic fungus Thermoascus crustaceus: production and industrial potential

Garbin

Garcia

Cavalheiro

et al. 2021

An. Acad. Bras. Ciênc.

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“…Finally, it is important to underline that different complex substrates with different compositions will induce distinct responses in the sense of inducing and/or repressing enzyme production. Different works in the literature using agro-industrial residues report these distinct behaviors depending on the composition and complexity of the media, not only for L. ramosa [1,2,4,5,13,14], but also for other filamentous fungi species and strains cultivated in residues [5,[9][10][11]21].…”

Section: Fatty Acidsmentioning

confidence: 99%

“…The use of agricultural residues as substrates provides an alternative for SSB because it adds value to these materials and assists in the mitigation of environmental problems [5]. Fruit wastes are rich in starch, cellulose, soluble sugars and organic compounds [6] and those of interest for SSB include apple, grape, kiwi, orange, pineapple, pequi, and guavira, among others [4,[7][8][9] The utilization of SSB to produce enzymes and protein enrichment has received great attention due to the low level of applied technology and efficiency in the conversion of substrates [4], with several applications widely reported in the literature, as amylase production to be used in the pharmaceutical and food industry, use of carboxymethylcellulase (CMCase), xylanase, and β-glucosidase have potential to hydrolyze plant cell wall e lipases are very important both from a physiological aspect, since they hydrolyze oils and fats into free fatty acids [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Biotransformation of fruit residues via solid state bioprocess using Lichtheimia ramosa

Silva

et al. 2020

SN Appl. Sci.

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Lichtheimia ramosa is a promising candidate fungus for solid state bioprocesses (SSB) due its rapid colonization. Fruit wastes present ideal conditions for fungal growth and biotransformation, which can promote the release of products of biotechnological interest. Thus, the aim of this work was to evaluate the mycelial growth and the enzymatic activities of L. ramosa in pineapple (Ananas comosus), orange (Citrus sinensis), mango (Mangifera indica), passion fruit (Passiflora edulis) and grape (Vitis vinifera) fruit wastes via SSB, and their influence in the generation of molecules with potential use. The SSB was evaluated in terms of capacity of biotransformation of the substrate (composition, protein enrichment, and fatty acid profile) and production of amylase, carboxymethylcellulase (CMCase), xylanase and lipase enzymes. Main cultivations were carried out at 30 °C for 30 days and every 5 days samples were taken and analyzed for microbiological content, proximal composition and enzymatic profile. Fatty acids were determined at day 0 (baseline) and at the end of the cultivations. L. ramosa grew well in all substrates up to the 25th day, except for on the orange residue, upon which development was slightly lower at this time. Protein enrichment was found in all substrates as follows: passion fruit (309.54%), pineapple (294.89%), mango (263.45%), orange (65.60%) and grapes (19.17%). Regarding enzymes, lipase was not synthetized in any of the substrates, though amylase, CMCase and xylanase were observed at different levels. The fatty acid profile varied from raw to cultivated substrates, indicating that L. ramosa can act in the synthesis and conversion of these acids. It was concluded that L. ramosa and the studied substrates are viable for SSB.

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Production and biochemical characterization of halotolerant β-glucosidase by Penicillium roqueforti ATCC 10110 grown in forage palm under solid-state fermentation

Neves

Menezes

Soares

et al. 2020

Biomass Conv. Bioref.

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Catalytic and thermodynamic properties of β-glucosidases produced byLichtheimia corymbiferaandByssochlamys spectabilis

Cited by 13 publications

References 37 publications

β-glucosidase from thermophilic fungus Thermoascus crustaceus: production and industrial potential

β-glucosidase from thermophilic fungus Thermoascus crustaceus: production and industrial potential

Biotransformation of fruit residues via solid state bioprocess using Lichtheimia ramosa

Production and biochemical characterization of halotolerant β-glucosidase by Penicillium roqueforti ATCC 10110 grown in forage palm under solid-state fermentation

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