Catalysis by site-specific recombinases

Stark, W. Marshall

doi:10.1016/0168-9525(92)90176-5

Cited by 245 publications

(130 citation statements)

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“…A topological analysis of recombination products can yield important information on the architecture of the synaptic complex and the mechanism of strand exchange employed by a recombinase (reviewed in Dröge & Cozzarelli 1992;Stark et al 1992). We therefore constructed plasmid pABD41 (Fig.…”

Section: Topological Analysis Of Recombination Productsmentioning

confidence: 99%

“…Resolution systems from various transposons have been studied in depth and the wealth of data suggests a very similar multistep model for these systems (reviewed in Sherratt 1989;Stark et al 1992;Grindley 1994;Stark & Boocock 1995;Oram et al 1995). In the first step, resolvase dimers bind to three subsites which together constitute a single, functional res site.…”

Section: Introductionmentioning

confidence: 99%

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The resolvase encoded by Xanthomonas campestris transposable element ISXc5 constitutes a new subfamily closely related to DNA invertases

Liu

Hühne

et al. 1998

Genes to Cells

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Background: Conservative site-specific recombination is responsible for the resolution of cointegrates which result during the transposition of class II transposable elements. Resolution is catalysed by a transposonencoded recombinase, resolvase, that belongs to a large family of recombinases, including DNA invertases. Resolvases and the related invertases are likely to employ similar reaction mechanisms during recombination. There are important differences, however. Resolvases require two accessory DNA binding sites within each of the two directly repeated recombination sites. Invertases instead need a host factor, Fis, and an enhancer type DNA sequence, in addition to two inversely orientated recombination sites.

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Section: Topological Analysis Of Recombination Productsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The resolvase encoded by Xanthomonas campestris transposable element ISXc5 constitutes a new subfamily closely related to DNA invertases

Liu

Hühne

et al. 1998

Genes to Cells

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“…A number of recent reviews have addressed a variety of mechanistic aspects of these reactions (Berg & Howe 1989;Landy 1989;Mizuuchi 1992a;Stark et al 1992). In this review, I will consider several aspects of the chemical steps of polynucleotidyl transfer by these recombination enzymes.…”

Section: Introductionmentioning

confidence: 99%

Polynucleotidyl transfer reactions in site‐specific DNA recombination

1997

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Site-specific DNA rearrangement reactions are widespread among organisms. They are used, for example, by vertebrates to boost immune response diversity, and in turn by parasitic organisms to evade the host immune system by surface antigen switching. Parasitic genetic elements ubiquitous to most organisms invade new host genomic sites by a variety of types of site-specific recombination. Polynucleotidyl transfer reactions are central to these DNA recombination reactions. The recombinase of each reaction system that 'catalyses' such chemical reactions at specific DNA sites are apparently designed to accomplish unique DNA geometrical specificity, or delicate control over the extent or direction of the reaction, with the sacrifice of protein turnover. Here we discuss our current understanding of several issues that relate to the polynucleotidyl transfer steps in several of the better studied site-specific recombination reactions.

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“…Recombination is catalysed by recombinase proteins that carry out DNA strand cleavage and transfer reactions at short DNA sequences known as recombination core sites (reviewed in Stark et al, 1992). Site-specific recombination often requires additional accessory proteins, which act at accessory sequences adjacent to the recombination core.…”

Section: Introductionmentioning

confidence: 99%

The ArcA/ArcB two‐component regulatory system of Escherichia coli is essential for Xer site‐specific recombination at psi

Colloms

Alén

Sherratt

1998

Molecular Microbiology

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SummaryTwo recombinases, XerC and XerD, act at the recombination sites psi and cer in plasmids pSC101 and ColE1 respectively. Recombination at these sites maintains the plasmids in a monomeric state and helps to promote stable plasmid inheritance. The accessory protein PepA acts at both psi and cer to ensure that only intramolecular recombination takes place. An additional accessory protein, ArgR, is required for recombination at cer but not at psi. Here, we demonstrate that the ArcA /ArcB two-component regulatory system of Escherichia coli, which mediates adaptation to anaerobic growth conditions, is required for efficient recombination in vivo at psi. Phosphorylated ArcA binds to psi in vitro and increases the efficiency of recombination at this site. Binding of ArcA to psi may contribute to the formation of a higher order synaptic complex between a pair of psi sites, thus helping to ensure that recombination is intramolecular.

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Catalysis by site-specific recombinases

Cited by 245 publications

References 0 publications

The resolvase encoded by Xanthomonas campestris transposable element ISXc5 constitutes a new subfamily closely related to DNA invertases

The resolvase encoded by Xanthomonas campestris transposable element ISXc5 constitutes a new subfamily closely related to DNA invertases

Polynucleotidyl transfer reactions in site‐specific DNA recombination

The ArcA/ArcB two‐component regulatory system of Escherichia coli is essential for Xer site‐specific recombination at psi

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