The resolvase encoded by <i>Xanthomonas campestris</i> transposable element IS<i>Xc5</i> constitutes a new subfamily closely related to DNA invertases

Liu, Chia‐Chen; Hühne, Rolf; Tu, Jenn; Lorbach, Elke; Dröge, Peter

doi:10.1046/j.1365-2443.1998.00182.x

Cited by 19 publications

(13 citation statements)

References 42 publications

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“…They are also found in many different bacterial species, presumably as a result of their association with plasmids or transposons. (21,30,44). The sources of the sequences are: Tn1403/In28, AF313472 and M59035; Tn1696/In4, U12338; Tn1412/In32, L36547; Tn21/In2, AF071413; R46/In1, M95287; pVS1/In0, U10456 and U49101; pSa/In6, U303471; pMG7, S78872; pCG4, AF164956; and DT104, AF071555 and AF261826.…”

Section: Discussionmentioning

confidence: 99%

Family of Class 1 Integrons Related to In4 from Tn 1696

Partridge

Recchia

Stokes

et al. 2001

Antimicrob Agents Chemother

127

122

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The class 1 integron In28, found in the multidrug resistance transposon Tn1403, was found to be located in the res site of the backbone transposon and is flanked by a 5-bp direct duplication, indicating that it reached this position by transposition. In28 has a backbone structure related to that of In4, but has lost internal sequences, including the sul1 gene, due to an IS6100-mediated deletion. In28 also lacks the partial copy of IS6100 found in In4 and contains different gene cassettes, blaP1, cmlA1, and aadA1. In1, the class 1 integron found in the multidrug resistance plasmid R46, is also located in a putative res site and belongs to the In4 group. In1 has a shorter internal deletion than In28 and has also lost one end. Additional integrons with structures related to In4 were also found in databases, and most of them had also lost either one end or internal regions or both. Tn610 belongs to this group.

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Section: Discussionmentioning

confidence: 99%

Family of Class 1 Integrons Related to In4 from Tn 1696

Partridge

Recchia

Stokes

et al. 2001

Antimicrob Agents Chemother

127

122

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“…These IS elements are of potential interest because the TnpR resolvase of ISXc5 represents a new subfamily of recombinases responsible for the resolution of co‐integrates of class II transposable elements, such as Tn 3 (Liu et al ., 1998). Intriguingly, pXAC64, which presumably carries a functional allele of pthA ( pthA4 ), also has a tnpA transposase gene that is 99.9% similar to the tnpA of Tn5044, which is in the Tn 3 subgroup of the Tn 3 family of transposases (Kholodii et al ., 2000).…”

Section: Introduction To Citrus Canker Disease: Molecular and Descripmentioning

confidence: 99%

Xanthomonas citri: breaking the surface

Brunings

Gabriel

2003

Molecular Plant Pathology

330

294

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SUMMARYTaxonomy: Bacteria; Proteobacteria, Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae, Xanthomonas.Microbiological properties: Gram-negative, obligately aerobic, straight rods, motile by a single polar flagellum, yellow pigment.Related species: X. campestris , X. axonopodis , X. oryzae , X. albilineans .Host range: Affects Rutaceous plants, primarily Citrus spp., Fortunella spp., and Poncirus spp., world-wide. Quarantined pathogen in many countries. Economically important hosts are cultivated orange, grapefruit, lime, lemon, pomelo and citrus rootstock.Disease symptoms: On leaves, first appearance is as oily looking, 2-10 mm, similarly sized, circular spots, usually on the abaxial surface. On leaves, stems, thorns and fruit, circular lesions become raised and blister-like, growing into white or yellow spongy pustules. These pustules then darken and thicken into a light tan to brown corky canker, which is rough to the touch. On stems, pustules may coalesce to split the epidermis along the stem length, and occasionally girdling of young stems may occur. Older lesions on leaves and fruit tend to have more elevated margins and are at times surrounded by a yellow chlorotic halo (that may disappear) and a sunken centre. Sunken craters are especially noticeable on fruit, but the lesions do not penetrate far into the rind. Defoliation and premature abscission of affected fruit occurs on heavily infected trees.

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“…The least divergent from the resolvase/invertases are transposon‐ and plasmid‐encoded recombinases that have an N‐terminal catalytic domain, a DNA binding motif and an additional, approximately 100 amino acids at the C‐terminus. Two of these, the resolvases from ISXc5 and that from Tn 5044 , from Xanthomonas campestris , have similar properties to Tn3 and γδ resolvases (Liu et al , 1998; Kholodii et al , 2000). The C‐terminal 100 amino acids in the ISXc5‐encoded resolvase are not essential for activity but the analogous region from the Tn 5044 resolvase is required, albeit function unknown (Kholodii et al , 2000; Schneider et al , 2000).…”

Section: Structural and Functional Variations To The Resolvase/invertmentioning

confidence: 98%

Diversity in the serine recombinases

Smith¹,

Thorpe

2002

Molecular Microbiology

267

278

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Summary Most site‐specific recombinases fall into one of two families, based on evolutionary and mechanistic relatedness. These are the tyrosine recombinases or λ integrase family and the serine recombinases or resolvase/invertase family. The tyrosine recombinases are structurally diverse and functionally versatile and include integrases, resolvases, invertases and transposases. Recent studies have revealed that the serine recombinase family is equally versatile and members have a variety of structural forms. The archetypal resolvase/invertases are highly regulated, only affect resolution or inversion and they have an N‐terminal catalytic domain and a C‐terminal DNA binding domain. Phage‐encoded serine recombinases (e.g. φC31 integrase) cause integration and excision with strictly controlled directionality, and have an N‐terminal catalytic domain but much longer C‐terminal domains compared with the resolvase/invertases. This high molecular weight group also contains transposases (e.g. TnpX from Tn4451). Other transposases, which belong to a third structurally different group, are similar in size to the resolvase/invertases but have the DNA binding domain N‐terminal to the catalytic domain (e.g. IS607 transposase). These three structural groups represented by the resolvase/invertases, the large serine recombinases and relatives of IS607 transposase correlate with three major groupings seen in a phylogeny of the catalytic domains. These observations indicate that the serine recombinases are modular and that fusion of the catalytic domain to unrelated sequences has generated structural and functional diversity.

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The resolvase encoded by Xanthomonas campestris transposable element ISXc5 constitutes a new subfamily closely related to DNA invertases

Cited by 19 publications

References 42 publications

Family of Class 1 Integrons Related to In4 from Tn 1696

Family of Class 1 Integrons Related to In4 from Tn 1696

Xanthomonas citri: breaking the surface

Diversity in the serine recombinases

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