Catalysis-based specific detection and inhibition of tyrosinase and their application

Qu, Yunwei; Qu, Zhan; Du, Shubo; Ding, Yang; Fang, Bin; Du, Wei; Wu, Qiong; Yu, Hai‐Dong; Li, Lin; Huang, Wei

doi:10.1016/j.jpha.2020.07.004

Cited by 39 publications

(26 citation statements)

References 81 publications

(105 reference statements)

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“…During the monophenolase cycle, to form E oxy -monophenol complex (E oxy M), the oxygen atom on the deprotonated monophenol is coordinated with the coppers of E oxy . Then, the phenol is o-hydroxylated to generate E met -diphenol (D) complex (E met D) [26,35]. Reducing agents could well draw the E met into the E deoxy , with concomitant oxidation to the corresponding o-quinone [36].…”

Section: Structural Feature and Catalytic Mechanism Of Tyrmentioning

confidence: 99%

Spectrophotometric Assays for Sensing Tyrosinase Activity and Their Applications

et al. 2021

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Tyrosinase (TYR, E.C. 1.14.18.1), a critical enzyme participating in melanogenesis, catalyzes the first two steps in melanin biosynthesis including the ortho-hydroxylation of L-tyrosine and the oxidation of L-DOPA. Previous pharmacological investigations have revealed that an abnormal level of TYR is tightly associated with various dermatoses, including albinism, age spots, and malignant melanoma. TYR inhibitors can partially block the formation of pigment, which are always used for improving skin tone and treating dermatoses. The practical and reliable assays for monitoring TYR activity levels are very useful for both disease diagnosis and drug discovery. This review comprehensively summarizes structural and enzymatic characteristics, catalytic mechanism and substrate preference of TYR, as well as the recent advances in biochemical assays for sensing TYR activity and their biomedical applications. The design strategies of various TYR substrates, alongside with several lists of all reported biochemical assays for sensing TYR including analytical conditions and kinetic parameters, are presented for the first time. Additionally, the biomedical applications and future perspectives of these optical assays are also highlighted. The information and knowledge presented in this review offer a group of practical and reliable assays and imaging tools for sensing TYR activities in complex biological systems, which strongly facilitates high-throughput screening TYR inhibitors and further investigations on the relevance of TYR to human diseases.

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Section: Structural Feature and Catalytic Mechanism Of Tyrmentioning

confidence: 99%

Spectrophotometric Assays for Sensing Tyrosinase Activity and Their Applications

et al. 2021

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“…No. ab252899) following colorimetric method Qu et al [16], Serum copper was determined using (Copper Assay Kit, Sigma-Aldrich , USA, Cat. No.…”

Section: Biochemical Determinationsmentioning

confidence: 99%

Effect of Copper Sulphate Pollution and its Antidote Penicillamine on Liver and Serum Markers of Albino Rats

Fawzy

Ahmed

Khamis

et al. 2021

Zagazig Veterinary Journal

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Copper is an essential trace element and is required for many metabolic functions. The present study was designed to study the negative impact of excess copper sulphate (Cuso4) on liver of albino rats and studying how its antidote d-penicillamine play role in the development of its side effects. Seventy albino rats were divided into seven equal groups each containing 10 rats (G1:control group received distilled water) ; (G2: 0.1 LD50 of CuSO4) ; (G3: 0.2 LD50 of CuSO4) ; (G4: 0.4 LD50 of CuSO4) ; (G5: 0.1 LD50 of CuSO4 +100 mg/kg/day of penicillamine) ; (G6: LD50 of CuSO4.+100 mg/kg/day of penicillamine) and (G7: LD50 of CuSO4+ 100 mg/kg/day of penicillamine) for 30 days. At the end of the experiment all rats were sacrificed, and blood samples and liver tissues were collected for biochemical and molecular assaying. The result showed that administration of copper sulphate with different levels induced a significant increase in fasting blood glucose level (FBG), lipid peroxidation marker (MDA), serum copper level, white blood cells (WBCs) and serum tyrosinase activity, but a significant decrease in total antioxidant activity (TAC), Hb and platelet count. Moreover, copper sulphate administration elicited a significant (P ˂ 0.05) downregulation of cytochrome c oxidase (Cyto co) and glucose -6-phosphate dehydrogenase (G6PD). It could be approved that d-penicillamine (DPA) can decrease the negative impact of copper sulphate on hepatic tissues and serum enzymes. DPA can reduce hepatotoxicity and oxidative stress caused by copper pollution.

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“…Melanogenesis refers to the production of melanin by melanocytes. The absence of melanocytes or loss of pigmentation causes skin diseases such as vitiligo and albinism [1]. Currently, the most preferred treatments to relieve skin damages are skin grafts, lasers, vitamin D analogs, and steroids.…”

Section: Introductionmentioning

confidence: 99%

Linarin enhances melanogenesis in B16F10 cells via MAPK and PI3K/AKT signaling pathways

Kang

Hyun

2021

JABC

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In this study, we discovered for the first time that linarin, a flavonoid compound, enhances melanin biosynthesis in B16F10 cells, and subsequently elucidated the underlying mechanism of linarin-induced melanogenesis. Linarin showed no cytotoxicity at a concentration of 42 μM and significantly increased intracellular tyrosinase activity and melanin content in B16F10 cells. Mechanistic analysis showed that linarin increased the expression of tyrosinase, tyrosinase-related protein 1 (TRP-1), and microphthalmia-associated transcription factor (MITF) that are related to melanogenesis. Moreover, linarin decreased the phosphorylation of extracellular signal-regulated kinase (ERK) and protein kinase B (AKT). Finally, we evaluated the effect of the structure-activity relationship of linarin and its aglycone on melanogenesis. The results indicated that linarin enhances the expression of melanogenic proteins by activating MITF expression via the modulation of mitogen-activated protein kinase (MAPK), phosphatidylinositol 3-kinase (PI3K), and protein kinase B signaling pathways in B16F10 cells, thereby enhancing melanogenesis.

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Catalysis-based specific detection and inhibition of tyrosinase and their application

Cited by 39 publications

References 81 publications

Spectrophotometric Assays for Sensing Tyrosinase Activity and Their Applications

Spectrophotometric Assays for Sensing Tyrosinase Activity and Their Applications

Effect of Copper Sulphate Pollution and its Antidote Penicillamine on Liver and Serum Markers of Albino Rats

Linarin enhances melanogenesis in B16F10 cells via MAPK and PI3K/AKT signaling pathways

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