Catalase: its effect on microbial enumeration

Martin, Scott E.; Flowers, Russell S; Ordal, Z. John

doi:10.1128/aem.32.5.731-734.1976

Cited by 120 publications

(80 citation statements)

References 18 publications

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“…The addition of catalase to the surface of selective medium plates also stimulated a growth of physically or chemically stressed (injured) microorganisms. It is reported that heat-injured cells had a decreased catalase activity and supplemented catalase acted by preventing the accumulation of hydrogen peroxide in or around injured cells [19]. It is proposed that a sudden transfer of injured or starved cells to nutrientrich agar at temperatures optimal for enzyme activities initiates an imbalance in metabolism, inducing a near instantaneous production of superoxide and free radicals.…”

Section: Discussionmentioning

confidence: 99%

Resuscitation of viable but nonculturable cells of Vibrio parahaemolyticus induced at low temperature under starvation

Mizunoe

2000

FEMS Microbiology Letters

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Vibrio parahaemolyticus is known to exist in a viable but nonculturable state when incubated at low temperature under starvation. It has long been debated whether the culturable cells which appear after temperature upshift are the result of true resuscitation or regrowth of a few residual culturable cells. Starved V. parahaemolyticus cells at 4°C reached the nonculturable stage in about 12 days. The true resuscitation of nonculturable cells of V. parahaemolyticus occurred after spreading them onto an agar medium supplemented with H2O2‐degrading compounds such as catalase or sodium pyruvate. The proposed method may be applicable to detecting the enteropathogen from environmental samples.

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Section: Discussionmentioning

confidence: 99%

Resuscitation of viable but nonculturable cells of Vibrio parahaemolyticus induced at low temperature under starvation

Mizunoe

2000

FEMS Microbiology Letters

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“…Thus, we reasoned that, if catalase activity was important in the VBNC response in V. vulnificus, then deletion of OxyR activity should result in a cell which would become nonculturable, similar to that observed in the VBNC state, even at ambient temperature. This would be due to the presence of H 2 O 2 in routine culture media, a fact which has been shown in a number of studies [21,22], or during metabolism of the cells when exposed to the high nutrient medium, as suggested by Bloomfield et al [11].…”

Section: Introductionmentioning

confidence: 91%

Role of catalase and oxyR in the viable but nonculturable state of Vibrio vulnificus

Kong

Bates

Hülsmann

et al. 2004

FEMS Microbiology Ecology

136

112

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Vibrio vulnificus has proven difficult to culture from water or shellfish during winter months, which is attributed to the viable but nonculturable (VBNC) state. Because reactive oxygen species were found to be involved in the low temperature-induced entrance of V. vulnificus into this state, we generated an oxyR mutant which lacks catalase activity. This strain is nonculturable on solid media even at ambient temperature, due to the presence of H(2)O(2) in such media. Low temperature incubation of the parent resulted in loss of catalase activity, making the cells H(2)O(2) sensitive, and paralleling the loss of culturability (entry into the VBNC state). Thus, cells of V. vulnificus in the VBNC state are likely exhibiting this response to low in situ temperature and only when the artificial condition of laboratory culture is attempted are the cells nonculturable due to cold-induced loss of catalase activity. To our knowledge, this is the first study providing direct evidence for the metabolic basis of nonculturability and the viable but nonculturable state.

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“…1987), azelaic acid (Passi et al 1991), caffeine (Shi et al 1991), carnosine (Pavlov et al 1991;Salim-Hanna et al 1991), cimetidine (Uchida and Kawakishi 1990), dimethylsulphoxide (DMSO ; Cederbaum et al 1977;Repine et al 1981), dimethyl thiourea (Repine et al 1981) and thiourea (Cederbaum et al 1979). The hydrogen peroxide scavengers were bovine liver catalase (Martin et al 1976) and sodium pyruvate (Thompson et al 1951). The singlet oxygen quenchers were spermidine, spermine (Khan et al 1992) and d-alpha-tocopherol (Kaiser et al 1990).…”

Section: Reactive Oxygen Intermediate Scavengersmentioning

confidence: 99%

Oxygen tolerance estimates in Campylobacter species depend on the testing medium

Hodge

Krieg

1994

Journal of Applied Bacteriology

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Oxygen tolerance of the microaerophile Campylobacter jejuni subsp. jejuni varied with different brands of complex media which were used for plating the dilute cell suspensions. The tryptone component was one factor. With some tryptones growth occurred at 21% oxygen whereas with others there was no growth at oxygen levels of 15% or higher. A chemically-defined, agar-solidified plating medium was used to estimate the oxygen tolerance of Camp. jejuni subsp. jejuni, Camp. coli and Camp. fetus subsp. fetus, and also to assess the effect of added scavengers of reactive oxygen intermediates on the oxygen tolerance. Some scavengers such as allopurinol, azelaic acid, caffeine, cimetidine, TEMPOL and pyruvate enhanced oxygen tolerance markedly whereas others such as carnosine, dimethyl thiourea, spermidine and superoxide dismutase had little effect.

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Catalase: its effect on microbial enumeration

Cited by 120 publications

References 18 publications

Resuscitation of viable but nonculturable cells of Vibrio parahaemolyticus induced at low temperature under starvation

Resuscitation of viable but nonculturable cells of Vibrio parahaemolyticus induced at low temperature under starvation

Role of catalase and oxyR in the viable but nonculturable state of Vibrio vulnificus

Oxygen tolerance estimates in Campylobacter species depend on the testing medium

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