Oxygen tolerance estimates in Campylobacter species depend on the testing medium

Hodge, Jeffrey Paul; Krieg, Noel R.

doi:10.1111/j.1365-2672.1994.tb02817.x

Cited by 24 publications

(21 citation statements)

References 29 publications

(34 reference statements)

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“…Instead, our results confirm that the protective effect of pyruvate might be based on the ability of pyruvate to act as a quencher of ROI, as assumed in literature (Bolton et al. 1984; Hodge and Krieg 1994). The measured decreased KatA activity in the presence of pyruvate in our study supports the suggestion of Giandomenico et al.…”

Section: Discussionsupporting

confidence: 90%

“…1978; Bolton et al. 1984; Hodge and Krieg 1994). However, these studies were performed on plates or in flasks, in which the cultivation conditions were only given as the percentage of oxygen in the gaseous phase.…”

Section: Discussionmentioning

confidence: 99%

“…1984; Fraser et al. 1992; Hodge and Krieg 1994). These compounds are thought to act as quenchers of ROI, but the exact mechanism is not yet clear.…”

Section: Introductionmentioning

confidence: 99%

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Pyruvate relieves the necessity of high induction levels of catalase and enables Campylobacter jejuni to grow under fully aerobic conditions

Arends

Snoep

Zwietering

et al. 2008

Lett Appl Microbiol

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Aims: Several cases of campylobacteriosis reported worldwide seemingly conflict with the strict growth requirements and sensitivity to environmental stress of Campylobacter jejuni. In this study, the need for a micro‐aerobic environment [dissolved oxygen tension (DOT): 0·1–90%; 100% air saturation)] and the adaptive responses to oxygen stress were studied. Methods and Results: The growth of C. jejuni in continuous culture was assessed under different DOT in the presence or absence of pyruvate. In a medium without pyruvate, continuous cultures of C. jejuni showed typically micro‐aerobic behaviour and cells were unable to grow under fully aerobic conditions. However in the presence of pyruvate (25 mmol l−1), continuous cultures of C. jejuni were able to grow in a broad DOT range, varying from 0·1% to at least 90%, and the catalase activity was decreased. Conclusions: Addition of pyruvate results in the decrease in the concentration of hydrogen peroxide, which enables C. jejuni to grow aerobically. Significance and Impact of the Study: New information on the oxidative physiology of C. jejuni and its ability to grow aerobically in media supplemented with pyruvate is presented.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

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Pyruvate relieves the necessity of high induction levels of catalase and enables Campylobacter jejuni to grow under fully aerobic conditions

Arends

Snoep

Zwietering

et al. 2008

Lett Appl Microbiol

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“…Tempol enhanced the growth of the anaerobic bacteria Campylobacter species. These bacteria normally cannot grow in media with an O 2 content above 15% but tolerated high O 2 contents in the presence of tempol (Hodge & Krieg, 1994). Oral tempol given to mice for four months after injection with Leishmania amazonensis inhibited inducible NOS expression and NO derived oxidants and increased the parasite burden (Linares et al, 2008).…”

Section: Additional Actions Of Tempolmentioning

confidence: 99%

Effects of tempol and redox-cycling nitroxides in models of oxidative stress

Wilcox

2010

Pharmacology & Therapeutics

411

344

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Tempol is a redox cycling nitroxide that promotes the metabolism of many reactive oxygen species (ROS) and improves nitric oxide bioavailability. It has been studied extensively in animal models of oxidative stress. Tempol has been shown to preserve mitochondria against oxidative damage and improve tissue oxygenation. Tempol improved insulin responsiveness in models of diabetes mellitus and improved the dyslipidemia, reduced the weight gain and prevented diastolic dysfunction and heart failure in fat-fed models of the metabolic syndrome. Tempol protected many organs, including the heart and brain, from ischemia/reperfusion damage. Tempol prevented podocyte damage, glomerulosclerosis, proteinuria and progressive loss of renal function in models of salt and mineralocorticosteroid excess. It reduced brain or spinal cord damage after ischemia or trauma and exerted a spinal analgesic action. Tempol improved survival in several models of shock. It protected normal cells from radiation while maintaining radiation sensitivity of tumor cells. Its paradoxical pro-oxidant action in tumor cells accounted for a reduction in spontaneous tumor formation. Tempol was effective in some models of neurodegeneration. Thus, tempol has been effective in preventing several of the adverse consequences of oxidative stress and inflammation that underlie radiation damage and many of the diseases associated with aging. Indeed, tempol given from birth prolonged the life span of normal mice. However, presently tempol has been used only in human subjects as a topical agent to prevent radiation-induced alopecia.

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“…Oxygen tolerance of Campylobacter jejuni varied even with different brands of complex media as with some tryptones growth occurred at 21% O 2 whereas with other brands no growth occurred at 15% O 2 [7]. The reason for these differences is not known but Hodge and Krieg [7] have proposed the use of media devoid of protein digest products for testing oxygen tolerance. The presence of peptone and/or yeast extract in the PYGV medium may have increased oxygen tolerance of some of the groundwater isolates and thus resulted in the higher proportion of isolates growing aerobically in PYGV than in glucose medium.…”

Section: Discussionmentioning

confidence: 99%

Psychrotolerant and microaerophilic bacteria in boreal groundwater

Männistö

Puhakka

2002

FEMS Microbiology Ecology

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Growth temperature and microaerophily of 39 phylogenetically different isolates from boreal oxygen-deficient groundwater were studied. Based on growth temperatures, the isolates were mainly psychrotolerant bacteria as 35 grew at 2 degrees C and only three at 35 degrees C. Growth rates in the range of 4-35 degrees C fitted the Ratkowsky square root model well. Optimum temperatures of the groundwater isolates varied between 18 and 30 degrees C. In semisolid glucose and PYGV media, 59% and 28% of the isolates, respectively, preferred microaerophilic growth and 33% were catalase-negative. The microaerophilic isolates had the highest sensitivity to H(2)O(2) whereas sensitivity to the superoxide generator paraquat was similar among microaerophilic and aerobic isolates. The results show that the cold (6-8 degrees C) and oxygen-deficient groundwater harbors psychrotolerant and microaerophilic bacteria of different phylogenetic origins which are well adapted to their environment.

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Oxygen tolerance estimates in Campylobacter species depend on the testing medium

Cited by 24 publications

References 29 publications

Pyruvate relieves the necessity of high induction levels of catalase and enables Campylobacter jejuni to grow under fully aerobic conditions

Pyruvate relieves the necessity of high induction levels of catalase and enables Campylobacter jejuni to grow under fully aerobic conditions

Effects of tempol and redox-cycling nitroxides in models of oxidative stress

Psychrotolerant and microaerophilic bacteria in boreal groundwater

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