Catalase function in plants: a focus on Arabidopsis mutants as stress-mimic models

Abstract

Hydrogen peroxide (H(2)O(2)) is an important signal molecule involved in plant development and environmental responses. Changes in H(2)O(2) availability can result from increased production or decreased metabolism. While plants contain several types of H(2)O(2)-metabolizing proteins, catalases are highly active enzymes that do not require cellular reductants as they primarily catalyse a dismutase reaction. This review provides an update on plant catalase genes, function, and subcellular localization, with a focus on recent information generated from studies on Arabidopsis. Original data are presented on Arabidopsis catalase single and double mutants, and the use of some of these lines as model systems to investigate the outcome of increases in intracellular H(2)O(2) are discussed. Particular attention is paid to interactions with cell thiol-disulphide status; the use of catalase-deficient plants to probe the apparent redundancy of reductive H(2)O(2)-metabolizing pathways; the importance of irradiance and growth daylength in determining the outcomes of catalase deficiency; and the induction of pathogenesis-related responses in catalase-deficient lines. Within the context of strategies aimed at understanding and engineering plant stress responses, the review also considers whether changes in catalase activities in wild-type plants are likely to be a significant part of plant responses to changes in environmental conditions or biotic challenge.

Two Cytoplasmic Effectors of Phytophthora sojae Regulate Plant Cell Death via Interactions with Plant Catalases

“…However, catalases are usually enriched in the cytoplasm and peroxisomes (Mhamdi et al, 2010). We found that N. benthamiana NbCAT1 colocalized with a peroxisome marker protein (Nelson et al, 2007;Fig.…”

“…8F). On the one hand, CATs prevent PCD by detoxifying H 2 O 2 , which is supported by a large body of evidence Mhamdi et al, 2010). LSD1, a negative regulator of PCD, interacts with CATs to regulate light-dependent runaway PCD and hypersensitive-type PCD (Li et al, 2013).…”

“…Plant catalase, a type of peroxisomal enzyme decomposing H 2 O 2 , plays essential roles in maintaining H 2 O 2 homeostasis and regulating PCD in plant cells (Mhamdi et al, 2010; Fig. 8F).…”

Role of ROS and auxin in plant response to metal-mediated stress

“…Excess ROS accumulation leading to oxidative stress is also assayed in plants exposed to the adverse environmental conditions. 7,[17][18][19] For metal stress, many metals such as Cd, Cu, Fe, Zn, Hg, Mn and Al can induce the ROS production 11,13,[20][21][22] and ROS scavenging systems, including catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxide (APX) and glutathione reductase (GR) often work in the protection against excess ROS toxicity in plants. 11,13,17,18,23 Hg has negative effect on algae growth and stimulates ROS production.…”

Comparison of an antioxidant system in tolerant and susceptible wheat seedlings in response to salt stress

“…H 2 O 2 is a toxic ROS and has deleterious effects in plant tissue. It is scavenged by CAT and different classes of peroxidase (POD) (Mhamdi et al, 2010). Other enzymes that are very important in the ROS scavenging system and function in the ascorbate-glutathione cycle are glutathione reductase (GR), monodehydroascorbate reductase (MDHAR; EC 1.6.5.4) and dehydroascorbate reductase (DHAR; EC 1.8.5.1) (Foyer & Noctor, 2011).…”