Catabolism of l-Arginine by Pseudomonas aeruginosa

Comparative studies on the degradation of guanidino and ureido compounds by Pseudomonas

“…The same diversity appears in the degradation of ureido compounds : ureohydrolysis is observed for allantoic acid and ureidoglycine; hydrolysis of the ureido group to C 0 2 and ammonia occurs for carbamoylputrescine (Mercenier et al, 1980 ;Stalon & Mercenier, 1984), carbamoylalanine (Carvaca & Grisolia, 1958 ;Campbell, 1960) and carbamoylsarcosine (Kim et al, 1976;Shimizu et al, 1986); phosphorolysis is observed for citrulline in Pseudornonas of group I having the arginine deiminase pathway and in other bacterial systems for carbamoylputrescine (Wargnies et al, 1979) and carbamoyloxamate (Vander Wauven et al, 1986). …”

“…Creatine amidinohydrolase (EC 3.. 5.3.3) was assayed in the presence of 100 mM-Trislmalate buffer, pH 8.0, and 25 mM-creatine. Agmatine deiminase (EC 3.5.3.12) was assayed under the conditions given by Mercenier et al (1980), but with 20 mwagmatine instead of 10 mM.…”

Diguanidinobutane (Arcaine) Degradation inRhodococcussp. C-x

“…The agmatine deiminase reaction was done under the conditions similar to those described by Mercenier et al 7 ) at the agmatine concentration of 6.0 mM. The reaction was stopped by the addition of 0.25 ml of 3 N HCI to the reaction mixture (1.0 ml).…”

“…done under conditions silimar to those described by Mercenier et al 7 ) at the carbamoylputrescine concentration of 3.0 mM. The reaction was stopped with HCI, the mixture was neutralized as described above, and then the ammonia formed was measured with alanine dehydrogenase.…”

Function of the Arginine Oxygenase Pathway in Utilization ofL-Arginine-related Compounds inArthrobacter globiformisandBrevibacterium helvolum

“…L-Citrulline, agmatine monohydrochloride, lithium carbamoylphosphate, barium argininosuccinate, 4;-guanidinobutyrate, bovine liver arginase (150 units/mg protein), aminooxyacetic acid (carboxymethoxylamine) hemihydrochloride, and gabaculine (3-amino-2,3-dihydrobenzoic acid) hydrochloride were purchased from Sigma (St. Louis, U.S.A.). 4-Aminobutyraldehyde was prepared from the diethylacetal (Aldrich Chemical Co., Milwaukee, U.s.A.) as described previously.l2) 4-Guanidinobutyramide was synthesized as described previously.lO) Carbamoylputrescine was synthesized from putrescine by the method of Mercenier et al 13 ) Peroxidase was the product of Toyobo Co. Ltd. (Osaka, Japan). Partially purified guanidinobutyramidase of B. helvolum lO ) and guanidinobutyrase of Pseudomonas sp.…”