Catabolic enzyme activities during biodegradation of three-ring PAHs by novel DTU-1Y and DTU-7P strains isolated from petroleum-contaminated soil

Sakshi, .; Singh, Santosh Kumar; Haritash, A. K.

doi:10.1007/s00203-021-02297-4

Cited by 32 publications

(2 citation statements)

References 36 publications

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“…Wang et al used an engineered microbial consortium composed of 4 bacteria and 8 fungi to degrade 75 mg/L of phenanthrene in 28 days, but the degradation ratio was only 67% . Pugazhendi et al also reported using a consortium of bacteria and fungi to degrade 200 mg/L of phenanthrene, whereby the degradation ratio reached 58% and 91% within 42–46 days. , Singh et al used Kocuria flava and Rhodococcus pyridinivorans degrade 10 mg/L of phenanthrene in 15 days, and the degradation ratio was only 61.32% . These degradation systems are composed of bacteria mixed with fungi, and the metabolism of different species is significantly different, which makes the system less predictable and controllable.…”

Section: Results and Discussionmentioning

confidence: 99%

Artificial Consortium of Three E. coli BL21 Strains with Synergistic Functional Modules for Complete Phenanthrene Degradation

et al. 2021

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Polycyclic aromatic hydrocarbons (PAHs) are highly toxic and persistent organic pollutions that can accumulate in the environment. In this study, an aromatic ring cleavage module, a salicylic acid synthesis module, and a catechol metabolism module were respectively constructed in three Escherichia coli BL21 strains. Subsequently, the engineered strains were cocultured as an artificial consortium for the biodegradation of phenanthrene, a typical PHA. Single factor experiments and response surface methodology were used to identify the optimal degradation conditions, including an inoculation interval of 6 h, inoculation ratio of 1:1:1, and IPTG concentration of 2 mM. Under these conditions, the 7-day degradation ratio of 100 mg/L phenanthrene reached 72.67%. Moreover, the engineered Escherichia coli BL21 strains showed good phenanthrene degradation ability at substrate concentrations 10 mg/L up to 500 mg/L. Enzyme activity assays combined with gas chromatography–mass spectrometry measurements confirmed that the three engineered strains behaved as a synergistic consortium in the phenanthrene degradation process. Based on the analysis of the key metabolites, the engineered bacteria were supplemented at 7-day intervals in batches so that each engineered strain maintained its optimal degradation ability. The 21-day degradation ratio finally reached 90.66%, which was much higher than what was observed with simultaneous inoculation. These findings suggest that the three engineered strains with separate modules constructed in this study offer an attractive solution for removing PAHs from the environment.

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Section: Results and Discussionmentioning

confidence: 99%

Artificial Consortium of Three E. coli BL21 Strains with Synergistic Functional Modules for Complete Phenanthrene Degradation

et al. 2021

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“…Microbially enhanced remediation is a promising technique for alleviating site pollution. Numerous studies have shown that bioaugmentation may enhance the bioremediation of PAHs in different environments, including water, sediment and soil (Sakshi et al 2021a(Sakshi et al , 2021b. However, the coexistence of heavy metals affects the bioremediation of PAHs pollution.…”

Section: Introductionmentioning

confidence: 99%

Synergistic effects of a functional bacterial consortium on enhancing phenanthrene biodegradation and counteracting rare earth biotoxicity in liquid and slurry systems

Wang

Liu

Zhang

et al. 2022

Letters in Applied Microbiology

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Significance and Impact of the Study: This study focused on the effect of rare earth (REs) on the biodegradation of polycyclic aromatic hydrocarbons (PAHs). The RE ions Ce 3+ and Y 3+ inhibited Moraxella osloensis CFP312 from degrading phenanthrene without affecting its glucose utilization. This inhibition effect can be relieved through co-cultivation with Bacillus subtilis MSP117, which has high adsorption capacity for RE ions in liquid and slurry systems. MSP117 adsorbed and fixed RE ions on its cell surfaces, thereby reducing the bioavailability of RE ions. This study provides a feasible way for the bioremediation of the co-pollution of RE and organic pollutants.

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Unveiling degradation mechanism of PAHs by a Sphingobium strain from a microbial consortium

Zhang¹,

Liu

Dai

et al. 2022

mLife

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Polycyclic aromatic hydrocarbons (PAHs) are a class of persistent pollutants with adverse biological effects and pose a serious threat to ecological environments and human health. The previously isolated phenanthrene‐degrading bacterial consortium (PDMC) consists of the genera Sphingobium and Pseudomonas and can degrade a wide range of PAHs. To identify the degradation mechanism of PAHs in the consortium PDMC, metagenomic binning was conducted and a Sphingomonadales assembly genome with 100% completeness was obtained. Additionally, Sphingobium sp. SHPJ‐2, an efficient degrader of PAHs, was successfully isolated from the consortium PDMC. Strain SHPJ‐2 has powerful degrading abilities and various degradation pathways of high‐molecular‐weight PAHs, including fluoranthene, pyrene, benzo[a]anthracene, and chrysene. Two ring‐hydroxylating dioxygenases, five cytochrome P450s, and a pair of electron transfer chains associated with PAH degradation in strain SHPJ‐2, which share 83.0%–99.0% similarity with their corresponding homologous proteins, were identified by a combination of Sphingomonadales assembly genome annotation, reverse‐transcription quantitative polymerase chain reaction and heterologous expression. Furthermore, when coexpressed in Escherichia coli BL21(DE3) with the appropriate electron transfer chain, PhnA1B1 could effectively degrade chrysene and benzo[a]anthracene, while PhnA2B2 degrade fluoranthene. Altogether, these results provide a comprehensive assessment of strain SHPJ‐2 and contribute to a better understanding of the molecular mechanism responsible for the PAH degradation.

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Catabolic enzyme activities during biodegradation of three-ring PAHs by novel DTU-1Y and DTU-7P strains isolated from petroleum-contaminated soil

Cited by 32 publications

References 36 publications

Artificial Consortium of Three E. coli BL21 Strains with Synergistic Functional Modules for Complete Phenanthrene Degradation

Artificial Consortium of Three E. coli BL21 Strains with Synergistic Functional Modules for Complete Phenanthrene Degradation

Synergistic effects of a functional bacterial consortium on enhancing phenanthrene biodegradation and counteracting rare earth biotoxicity in liquid and slurry systems

Unveiling degradation mechanism of PAHs by a Sphingobium strain from a microbial consortium

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