Cat-to-Human Orthopoxvirus Transmission, Northeastern Italy

Carletti, Fabrizio; Bordi, Licia; Castilletti, Concetta; Di, Antonino; Falasca, Laura; Gioia, Cristiana; Ippolito, Giuseppe; Zaniratti, S.; Beltrame, Anna; Viale, Pierluigi; Capobianchi, Maria Rosaria

doi:10.3201/eid1503.080813

Cited by 26 publications

(40 citation statements)

References 5 publications

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“…1 Our study reinforces the fact that coinfections with OPV and PPVs may occur in cattle not only involving PCPV but also BPSV. The high sequence identity and the circulation of CPXV in Italy in different animal species [5][6][7] lead us to conclude that the OPV DNA detected in domestic ruminants might be CPXV. This virus used to be enzootic in cattle; however, such infections have not been diagnosed over the last decades, while individual cases of cowpox have been repeatedly found in cats or exotic zoo animals that were responsible for human transmission.…”

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confidence: 99%

Evidence of zoonotic Poxviridae coinfections in clinically diagnosed papillomas using a newly developed mini-array test

et al. 2015

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Our study describes a newly developed mini-array test for the rapid detection of poxviruses in animals and humans. The method is based on detection that combines target nucleic acid amplification by polymerase chain reaction and specific hybridization, using enzyme-linked antibodies, allowing identification of zoonotic orthopoxviruses and parapoxviruses in animal and human biological samples. With 100% specificity, the test rules out the possibility of cross-reactions with viral agents causing look-alike diseases. The assay was employed in the field to investigate the causes of several outbreaks of a malignant proliferative skin disease that affected domestic ruminants in Sicily during 2011–2014. Due to specific aspects of the lesions, the animals were clinically diagnosed with papillomatosis. The mini-array test allowed the identification of coinfections caused by more than 1 viral species belonging to the Parapoxvirus and Orthopoxvirus genera, either in goats or in cattle. Our study suggests that the so-called “papillomatosis” can be the result of multiple infections with epitheliotropic viruses, including zoonotic poxviruses that cannot be properly identified with classical diagnostic techniques.

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confidence: 99%

Evidence of zoonotic Poxviridae coinfections in clinically diagnosed papillomas using a newly developed mini-array test

et al. 2015

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“…PCR in combination with RFLP assay [36][37][38] and sequencing 39 were proposed for CPXV identification. However, both these methods are not free from shortcomings.…”

Section: Introductionmentioning

confidence: 99%

Development of real-time PCR assay for specific detection of cowpox virus

Gavrilova¹,

Щербаков²,

Maksyutov³

et al. 2010

Journal of Clinical Virology

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“…Thus, worldwide, almost all individuals below 33 years of age have no immunity against smallpox and other human orthopoxvirus infections, resulting in numerous reported outbreaks of the human diseases caused by the zoonotic OPVs, including MPXV and CPXV (Campe et al, 2009;Carletti et al, 2009;Ninove et al, 2009). Therefore, there is a need to develop a rapid and reliable method for CPXV detection (Gavrilova et al, 2010).…”

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confidence: 99%