Cat’s claw inhibits TNFα production and scavenges free radicals: role in cytoprotection

Sandoval, Manuel; Charbonnet, Randi M; Okuhama, Nataly N.; Roberts, Jarod; Křenová, Zdenka; Trentacosti, Ann Marie; Miller, Mark J.S.

doi:10.1016/s0891-5849(00)00327-0

Cited by 147 publications

(141 citation statements)

References 32 publications

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“…• (Sandoval et al 2000). Ju et al (2004) reported the extract from the dried bark of Betula platyphylla var.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant activity of phenolic fractions of Mallotus philippinensis bark extract

Arfan¹,

Amin²,

Karamać³

et al. 2009

Czech J. Food Sci.

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Phenolic compounds were extracted from Mallotus philippinensis bark using methanol. Six fractions (I-VI) were separated from the extract on a Sephadex LH-20 column using ethanol and acetone-water as the mobile phases and were evaluated for their total antioxidant activity, antiradical activity against DPPH• (2,2-diphenyl-1-picrylhydrazyl radical), and reducing power. The total phenolics and tannin contents in the fractions were determined. The content of total phenolics in the fractions ranged from 54 mg/g (fraction I) to 927 mg/g (fraction VI). Condensed tannins were detected in fractions II-VI. Total antioxidant activity (TAA) of phenolic fractions of Mallotus philipinensis bark extract ranged from 0.58 mmol Trolox/g (fraction I) to 6.82 mmol Trolox/g (fraction IV). Fraction IV also showed the strongest antiradical activity against DPPH • and reducing power. Several phenolic constituents in the fractions were detected by RP-HPLC using a gradient solvent system with UV-DAD detection.

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“…• (Sandoval et al 2000). Ju et al (2004) reported the extract from the dried bark of Betula platyphylla var.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant activity of phenolic fractions of Mallotus philippinensis bark extract

Arfan¹,

Amin²,

Karamać³

et al. 2009

Czech J. Food Sci.

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“…Aguilar et al y Aquino et al, demostraron que los extractos etanólico y metánolico de UT reducen la inflamación en el edema plantar (7,16) . Sandoval et al, demostraron que la inhibición de la inflamación se relaciona con la disminución de TNF-α y es mediado por la inhibición de NFκB (17) . Fazio et al, demostraron que la UT reduce tumores de melanoma, metástasis pulmonar e inhibe TNF-α e IL-6 (8) .…”

Section: Introductionunclassified

Poblaciones linfocitarias, células dendríticas y perfil de citoquinas en ratones con melanoma tratados con Uncaria tomentosa

Lozada-Requena

Núñez

Álvarez

et al. 2015

Rev Peru Med Exp Salud Publica

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RESUMENObjetivos. Evaluar el efecto inmunomodulador sobre poblaciones linfocitarias, células dendríticas (DC), citoquinas Th1/Th2/Th17 (T-helper) e inflamatorias en el ámbito sistémico y/o en el microambiente tumoral de ratones con o sin melanoma. Materiales y métodos. Se obtuvieron muestras de sangre periférica y/o de tumores primarios de ratones con melanoma B16 tratados o no con un extracto hidroalcohólico de Uncaria tomentosa (UT) con 5,03% de alcaloides oxindólicos pentacíclicos (UT-POA) obtenido de la corteza de la planta. Todos los ensayos de medición de células y citoquinas fueron realizados por citometría de flujo. Resultados. UT-POA a nivel sistémico incrementa la relación CD4/CD8a (Cluster of Differenciation), mientras que la activación celular es inversamente proporcional; incrementa la proporción de DCm (DC mieloides); induce un perfil Th1 proinflamatorio y reduce la respuesta Th17. TNF-a (tumor necrosis factor alpha) y IL-17A (interleuquina) correlacionan positiva y negativamente con la relación CD4/CD8a. Conclusiones. El incremento de Th1 (TNF-a) puede tener como consecuencia el incremento de linfocitos CD4 o la activación de macrófagos M1. Aunque UT-POA muestra un incremento de DCm, este no es dosis-dependiente. La disminución de Th17 (IL-17A) puede favorecer el funcionamiento de los linfocitos CD8a. UT-POA muestra mejores efectos inmunomoduladores en el ámbito sistémico que intratumoral. Palabras clave: Uncaria tomentosa; Activación de linfocitos; Células dendríticas; Melanoma (fuente: DeCS BIREME). LYMPHOCYTE SUBSETS, DENDRITIC CELLS AND CYTOKINE PROFILES IN MICE WITH MELANOMA TREATED WITH Uncaria tomentosa ABSTRACT Objectives.To evaluate the immunomodulatory effect on lymphocyte subsets, dendritic cells (DC), Th1 / Th2 / Th17 and inflammatory cytokines on systemic level and/or in the tumor microenvironment of mice with or without melanoma. Materials and methods: Peripheral blood and/or primary tumors samples were obtained of mice with B16 melanoma treated or not with a hydroalcoholic extract of Uncaria tomentosa (UT) with 5.03% of pentacyclic oxindole alkaloids (UT-POA) obtained from the bark of the plant. All cell assays and cytokine measurements were performed by flow cytometry. Results. UT-POA systemically increased CD4/CD8a relation while cell activation was inversely proportional; increased the proportion of DCm; induced a pro-inflammatory Th1 profile and reduced Th17 response. TNF-α and IL-17A positively and negatively correlated with CD4/CD8a relation. Conclusions. The increase of Th1 (TNF-α) may result in the increase of CD4 or M1 macrophage activation. Although UT-POA shows increased DCm, is not dose-dependent. Th17(IL-17A) decreased can support the function of CD8a lymphocytes. UT-POA shows better systemic immunomodulatory effects than intratumoral.

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“…27 Uncaria tomentosa is a medicinal plant used over the centuries by the indigenous civilization of the peruvian rainforest, as an alternative treatment for different diseases. Studies performed in the last decades proved its antiinflamatory, 5,8,17,25 antineoplastic, 19,20 anticonceptive, 12 immunostimulant 18 and antioxidant 4,11,13 properties. Fitotherapy is a viable alternative for all health professionals in the prevention and treatment of several pathologies.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of Uncaria tomentosa against oral human pathogens

et al. 2007

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Uncaria tomentosa is considered a medicinal plant used over centuries by the peruvian population as an alternative treatment for several diseases. Many microorganisms usually inhabit the human oral cavity and under certain conditions can become etiologic agents of diseases. The aim of the present study was to evaluate the antimicrobial activity of different concentrations of Uncaria tomentosa on different strains of microorganisms isolated from the human oral cavity. Micropulverized Uncaria tomentosa was tested in vitro to determine the minimum inhibitory concentration (MIC) on selected microbial strains. The tested strains were oral clinical isolates of Streptococcus mutans, Staphylococcus spp., Candida albicans, Enterobacteriaceae and Pseudomonas aeruginosa. The tested concentrations of Uncaria tomentosa ranged from 0.25-5% in Müeller-Hinton agar. Three percent Uncaria tomentosa inhibited 8% of Enterobacteriaceae isolates, 52% of S. mutans and 96% of Staphylococcus spp. The tested concentrations did not present inhibitory effect on P. aeruginosa and C. albicans. It could be concluded that micropulverized Uncaria tomentosa presented antimicrobial activity on Enterobacteriaceae, S. mutans and Staphylococcus spp. isolates. Descriptors: Cat's claw; Contact inhibition; Bacteria; Candida albicans.Resumo: Uncaria tomentosa é uma planta medicinal usada por vários séculos pela população peruana como alternativa de tratamento para diversas doenças. Muitos microrganismos que usualmente não habitam a cavidade bucal humana podem se tornar agentes etiológicos de doenças sob certas condições. O objetivo deste estudo foi avaliar a atividade antimicrobiana de diferentes concentrações de Uncaria tomentosa sobre diferentes cepas de microrganismos isolados de cavidades bucais humanas. Uncaria tomentosa micropulverizada foi testada in vitro para determinar a concentração inibitória mínima (CIM) em isolados microbianos selecionados. Cepas de Streptococcus mutans, Staphylococcus spp., Candida albicans, Enterobacteriaceae e Pseudomonas aeruginosa avaliadas foram isoladas de cavidades bucais humanas. Foram preparadas as concentrações de Uncaria tomentosa entre 0.25 e 5% em ágar Müeller-Hinton. Uncaria tomentosa a 3% inibiu 8% de Enterobacteriaceae, 52% de S. mutans e 96% de Staphylococcus spp. As concentrações testadas não apresentaram efeito inibitório sobre P. aeruginosa e C. albicans. Concluiu-se que Uncaria tomentosa micropulverizada apresenta atividade antimicrobiana sobre cepas de Enterobacteriaceae, S. mutans e Staphylococcus spp.

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Cat’s claw inhibits TNFα production and scavenges free radicals: role in cytoprotection

Cited by 147 publications

References 32 publications

Antioxidant activity of phenolic fractions of Mallotus philippinensis bark extract

Antioxidant activity of phenolic fractions of Mallotus philippinensis bark extract

Poblaciones linfocitarias, células dendríticas y perfil de citoquinas en ratones con melanoma tratados con Uncaria tomentosa

Antimicrobial activity of Uncaria tomentosa against oral human pathogens

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