Cassava pulp enzymatic hydrolysis process as a preliminary step in bio-alcohols production from waste starchy resources

Virunanon, Chompunuch; Ouephanit, Chanika; Burapatana, Vorakan; Chulalaksananukul, Warawut

doi:10.1016/j.jclepro.2012.07.055

Cited by 64 publications

(26 citation statements)

References 25 publications

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“…These polysaccharides first have to be converted into sugars, in order to achieve high ethanol efficiencies and make the overall process economically viable. This is the case with wasted crop, cereal bran, cassava pulp, sago pith residues and brewery-spent grains, which have been proposed as low-cost materials for bioethanol, mainly by means of chemical pre-treatment, commercial cellulases, xylanase and amylases addition and subsequent fermentation [14][15][16][17][18][19]. The previously mentioned studies, though achieving promising results, demonstrate that the total exploitation of such substrates still needs to be addressed and that there is an opportunity to further increase the hydrolysis and fermentation yields from agricultural by-products containing different polysaccharides.…”

Section: Introductionmentioning

confidence: 99%

Utilisation of wheat bran as a substrate for bioethanol production using recombinant cellulases and amylolytic yeast

et al. 2015

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Wheat bran, generated from the milling of wheat, represents a promising feedstock for the production of bioethanol. This substrate consists of three main components: starch, hemicellulose and cellulose. The optimal conditions for wheat bran hydrolysis have been determined using a recombinant cellulase cocktail (RCC), which contains two cellobiohydrolases, an endoglucanase and a beta-glucosidase. The 10% (w/v, expressed in terms of dry matter) substrate loading yielded the most glucose, while the 2% loading gave the best hydrolysis efficiency (degree of saccharification) using unmilled wheat bran. The ethanol production of two industrial amylolytic Saccharomyces cerevisiae strains, MEL2[TLG1-SFA1] and M2n [TLG1-SFA1], were compared in a simultaneous saccharification and fermentation (SSF) for 10% wheat bran loading with or without the supplementation of optimised RCC. The recombinant yeasts. cerevisiae MEL2[TLG1-SFA1] and M2n[TLG1-SFA1] completely hydrolysed wheat bran's starch producing similar amounts of ethanol (5.3 +/- 0.14 g/L and 5.0 +/- 0.09 g/L, respectively). Supplementing SSF with RCC resulted in additional ethanol production of about 2.0 g/L. Scanning electron microscopy confirmed the effectiveness of both RCC and engineered amylolytic strains in terms of cellulose and starch depolymerisatio

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Section: Introductionmentioning

confidence: 99%

Utilisation of wheat bran as a substrate for bioethanol production using recombinant cellulases and amylolytic yeast

et al. 2015

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“…Além do ácido butírico, obteve-se uma produção de ácido láctico com o inóculo SM (1650,0 ± 580,0 mg L -1 ) e CM (3170,0 ± 1100,0 mg L -1 ) (Figura 5), mostrando que os consórcios tiveram potencial para produzir outros bioprodutos com interesse comercial. Virunanon et al (2013), utilizando como substrato efluente de mandioca e Clostridium acetobutyricum como inóculo, obtiveram produção de butanol de 850,0 mg L -1 e 1760,0 mg L -1 de etanol, a concentração final de ácido butírico foi de 2800,0 mg L -1 , também evidenciado no presente estudo.…”

Section: Produção De áCidos Orgânicosunclassified

Production Of Biofuel And Organic Acids For Adding Value To Cassava Wastewater

Chogi¹,

Araujo²,

Prado³

et al. 2020

Rev. Virtual Quim.

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Resíduos agroindustriais são ricos em açúcares que podem ser fermentáveis, sendo promissores na geração de produtos de alto valor agregado em processos biotecnológicos. Dentre estes produtos estão os ácidos orgânicos voláteis e álcoois. A fermentação acetona-butanol-etanol (ABE) vem sendo cada vez mais estudada de modo a aumentar a produção dos ácidos e solventes produzidos a partir destas fontes renováveis. Este trabalho teve como objetivo avaliar a produção de solventes e ácidos orgânicos a partir de dois substratos, glicose e água residual do processamento de farinha de mandioca (manipueira). As fermentações ABE em batelada foram realizadas com Clostridium beijerinckii (ATCC 10132)-CB, e três consórcios microbianos previamente submetidos a tratamento térmico, sendo estes o efluente de suinocultura-SM; lodo UASB da estação de tratamento de esgoto-UB; esterco bovino-CM. Enquanto que o inóculo CB obteve maior produção de butanol (1,03 g L-1) e etanol (2,31 g L-1) utilizando a manipueira como fonte de carbono, os demais inóculos exceto UB, produziram maiores quantidades de ácido butírico nas concentrações de 0,31 g L-1 (CM) e 0,13 g L-1 (SM) também com manipueira como fonte de carbono. Outros ácidos orgânicos também foram produzidos nestas fermentações. Os resultados deste estudo mostram a viabilidade do uso da manipueira como fonte de carbono na fermentação ABE para a produção de ácidos e solventes, além de promover uma forma de agregar valor a esses resíduos.

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“…Moreover, exogenous fibrolytic enzymes influenced rumen fermentation and improved feed efficiency of growing buffalo calves (Malik and Bandla 2010). Therefore, the fibrous structure in FCSR may be disrupted by exogenous enzyme (Sriroth et al 2000), producing a high yield of free sugar (Virunanon et al 2013) acting as an energy source for the benefit of rumen microbes.…”

Section: Carcass Characteristicsmentioning

confidence: 99%

Growth performance and carcass characteristics of feedlot Thai native × Lowline Angus crossbred steer fed with fermented cassava starch residue

Pilajun

Wanapat

2016

Trop Anim Health Prod

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Ten Thai native × Lowline Angus crossbred (50:50) steers were used in a completely randomized design to receive two treatments including concentrate containing cassava chips (Control) and fermented cassava starch residue (FCSR) as major carbohydrate sources. FCSR was prepared as yeast (Saccharomyces cerevisiae) inoculates with exogenous enzyme addition. All steers received ad libitum rice straw and concentrate in equal proportions (1:1). Replacement of cassava chip with FCSR increased neutral detergent fiber and acid detergent fiber intakes but decreased non-protein nitrogen intake of steers. Digestibility of dry matter, organic matter, and crude protein were decreased with replacement of cassava chip by FCSR; however, digested nutrients were similar between groups. Replacement of cassava chip in the concentrate with FCSR resulted in comparable growth performance and feed efficiency of the feedlot steers. Moreover, carcass characteristics in terms of yield and meat quality of the steers were not affected by cassava chips replaced by FCSR as a major carbohydrate source in the concentrate. In conclusion, replacement of cassava chip in the concentrate with FCSR decreased feed digestibility; however, it did not impact negatively on growth performance and carcass traits of feedlot Thai native × Lowline Angus crossbred steers.

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Cassava pulp enzymatic hydrolysis process as a preliminary step in bio-alcohols production from waste starchy resources

Cited by 64 publications

References 25 publications

Utilisation of wheat bran as a substrate for bioethanol production using recombinant cellulases and amylolytic yeast

Utilisation of wheat bran as a substrate for bioethanol production using recombinant cellulases and amylolytic yeast

Production Of Biofuel And Organic Acids For Adding Value To Cassava Wastewater

Growth performance and carcass characteristics of feedlot Thai native × Lowline Angus crossbred steer fed with fermented cassava starch residue

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