Caspase-8 controls the secretion of inflammatory lysyl-tRNA synthetase in exosomes from cancer cells

Kim, Sang Bum; Kim, Hye Rim; Park, Min-Chul; Cho, Seong-Min; Goughnour, Peter C.; Han, Daeyoung; Yoon, Ina; Kim, Youn Ha; Kang, Taehee; Song, Eunjoo; Kim, Pilhan; Choi, Hyosun; Mun, Ji Young; Song, Chihong; Lee, Sang‐Min; Jung, Hyun Suk; Kim, Sung Hoon

doi:10.1083/jcb.201605118

Cited by 79 publications

(82 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Plasticembedded samples for immunostaining were sectioned using an ultramicrotome (60~80-nm thickness). Sections collected using an EM grid were stained for specific antigens using antibody-conjugated gold nanoparticles (immunogold labeling) (Kim et al, 2017). In HPF-FS images, we confirmed that the shape of exosomes was round.…”

Section: Cryofixation Of Exosome-treated Cells and Cryo-tem Of Exosomesmentioning

confidence: 56%

Structural Analysis of Exosomes Using Different Types of Electron Microscopy

Choi

Mun

2017

Self Cite

View full text Add to dashboard Cite

Negative staining has been traditionally used for exosome imaging; however, the technique is limited to surface topology only and can cause staining artifacts. Therefore, to analyze the internal structure of exosomes, we employed a method of block preparation, thin sectioning, and electron tomography. In addition, an automatic serial sectioning technique with 15-nm thickness through focused ion beam was employed to observe the three-dimensional structure of exosomes of various sizes. Cryo-transmission electron microscopy revealed the near-to-native structure of exosomes.

show abstract

Section: Cryofixation Of Exosome-treated Cells and Cryo-tem Of Exosomesmentioning

confidence: 56%

Structural Analysis of Exosomes Using Different Types of Electron Microscopy

Choi

Mun

2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…Serum starvation of HCT116 colorectal carcinoma cells has been demonstrated to induce exosomal secretion of lysyl‐tRNA synthase (KRS) after dissociation of the multi‐tRNA synthetase complex . KRS sorting in exosomes was shown to involve caspase‐8 cleavage of KRS, thus unmasking a PDZ‐binding motif that could then interact with syntenin and Alix.…”

Section: Exosomes and Homeostasismentioning

confidence: 99%

Exosomes: Revisiting their role as “garbage bags”

Vidal

2019

Traffic

116

View full text Add to dashboard Cite

In recent years, the term “extracellular vesicle” (EV) has been used to define different types of vesicles released by various cells. It includes plasma membrane‐derived vesicles (ectosomes/microvesicles) and endosome‐derived vesicles (exosomes). Although it remains difficult to evaluate the compartment of origin of the two kinds of vesicles once released, it is critical to discriminate these vesicles because their mode of biogenesis is probably directly related to their physiologic function and/or to the physio‐pathologic state of the producing cell. The purpose of this review is to specifically consider exosome secretion and its consequences in terms of a material loss for producing cells, rather than on the effects of exosomes once they are taken up by recipient cells. I especially describe one putative basic function of exosomes, that is, to convey material out of cells for off‐site degradation by recipient cells. As illustrated by some examples, these components could be evacuated from cells for various reasons, for example, to promote “differentiation” or enhance homeostatic responses. This basic function might explain why so many diseases have made use of the exosomal pathway during pathogenesis.

show abstract

“…In addition, patients with high KRS expression in cancer cells accompanied by low or no KRS expression in inflammatory cells had significantly reduced survival rates. Conspicuously, Kim et al 110 systematically studied the secretion mechanism of KRS in colorectal carcinoma cells (Fig. 4).…”

Section: Arss and Tumor Immunitymentioning

confidence: 99%

Roles of aminoacyl-tRNA synthetases in immune regulation and immune diseases

Nie

Sun

et al. 2019

Cell Death Dis

View full text Add to dashboard Cite

Aminoacyl-tRNA synthetases (ARSs) play a vital role in protein synthesis by linking amino acids to their cognate transfer RNAs (tRNAs). This typical function has been well recognized over the past few decades. However, accumulating evidence reveals that ARSs are involved in a wide range of physiological and pathological processes apart from translation. Strikingly, certain ARSs are closely related to different types of immune responses. In this review, we address the infection and immune responses induced by pathogen ARSs, as well as the potential anti-infective compounds that target pathogen ARSs. Meanwhile, we describe the functional mechanisms of ARSs in the development of immune cells. In addition, we focus on the roles of ARSs in certain immune diseases, such as autoimmune diseases, infectious diseases, and tumor immunity. Although our knowledge of ARSs in the immunological context is still in its infancy, research in this field may provide new ideas for the treatment of immune-related diseases.

show abstract

Caspase-8 controls the secretion of inflammatory lysyl-tRNA synthetase in exosomes from cancer cells

Abstract: Lysyl-tRNA synthetase (KRS) can be released from cancer cells to cause inflammation, but the mechanism of KRS secretion is unknown. Kim et al. demonstrate that KRS is cleaved by caspase-8, which exposes a binding motif for syntenin and facilitates the secretion of KRS in exosomes.

Cited by 79 publications

References 52 publications

Structural Analysis of Exosomes Using Different Types of Electron Microscopy

Structural Analysis of Exosomes Using Different Types of Electron Microscopy

Exosomes: Revisiting their role as “garbage bags”

Roles of aminoacyl-tRNA synthetases in immune regulation and immune diseases

Contact Info

Product

Resources

About