Caspase-1 als Regulator der Autoinflammation bei rheumatischen Erkrankungen

Winkler, Stefan; Hedrich, Christian M.; Rösen‐Wolff, Angela

doi:10.1007/s00393-016-0077-3

Cited by 11 publications

(9 citation statements)

References 124 publications

(95 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Caspase-1 is a member of the Caspase family, and was the first to be identified ( 18 ). As the typical inflammation-associated Caspase, Caspase-1 is a key effector molecule during the inflammatory response, regulating the inflammatory reaction in the tumor microenvironment and the anti-tumor immunity of organisms ( 19 , 20 ). Recent studies demonstrated that Caspase-1 functions in apoptosis ( 21 , 22 ).…”

Section: Discussionmentioning

confidence: 99%

Expression of Caspase-1 in breast cancer tissues and its effects on cell proliferation, apoptosis and invasion

Sun¹,

Guo²

2018

Oncol Lett

View full text Add to dashboard Cite

The present study aimed to detect the expression of Caspase-1 in the tumor tissues and tumor-adjacent tissues of patients with breast cancer, and to investigate the effects of Caspase-1 on the proliferation, apoptosis and invasion of breast cancer cells. Reverse transcription-quantitative polymerase chain reaction was used to detect Caspase-1 mRNA expression in breast cancer tissues and tumor-adjacent tissues from patients. Additionally, the human breast cancer MDA-MB-231 cell line was treated with the Caspase-1 small molecule inhibitor Ac-YVAD-CMK, following which the changes to Caspase-1 protein expression were detected via western blotting. The MTT method detected the changes to cell proliferation, flow cytometry detected the rate of apoptosis, and a Transwell assay was employed to assess invasion. Caspase-1 mRNA expression was significantly decreased in the breast cancer tissues of patients, compared with in the tumor-adjacent tissues, a difference that was statistically significant (P<0.05). Treatment with the Ac-YVAD-CMK markedly decreased the protein expression of Caspase-1 in MDA-MB-231 cells, and the difference was statistically significant (P<0.05). Following this treatment of Ac-YVAD-CMK cells, the proliferation and invasion abilities markedly increased, while the apoptotic levels significantly decreased (P<0.05). In conclusion, the expression of Caspase-1 is low in breast cancer tissues, which may promote the proliferation and invasion of breast cancer cells and could be closely associated with the occurrence and development of breast cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

Expression of Caspase-1 in breast cancer tissues and its effects on cell proliferation, apoptosis and invasion

Sun¹,

Guo²

2018

Oncol Lett

View full text Add to dashboard Cite

show abstract

“…IL-18 is a novel cytokine with the similar structure of IL-1 and the analogous biological function of IL-12 [17]. Inactive IL-18 protein is cleaved by Caspase-1 to acquire activity and Caspase-1 has been found to regulate autoin ammation in the occurrence of rheumatic diseases, including AS [18]. In publication, IL-18 can facilitate Th1 cells to secret a number of cytokines, including IFN-γ, IL-2, GM-CSF and so on, and promote the proliferation of Th1 cells, too [19].…”

Section: Discussionmentioning

confidence: 99%

Interleukin-18 Polymorphisms Impose Considerable Impacts on Ankylosing Spondylitis Occurrence

Gou

Wang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: This research was conducted to explore the genetic association of interleukin-18 (IL-18) polymorphisms in promoter with ankylosing spondylitis (AS) in Chinese population using a case-control design.Methods: Polymerase chain reaction (PCR) and sequencing were used to complete the genotyping of IL-18 polymorphisms in 146 AS patients and 134 healthy controls. The genotype distribution of polymorphism was detected the status of Hardy-Weinberg equilibrium (HWE). The genotype and allele frequencies difference between the two groups was compared by chi-square test. Odds ratio (OR) with 95% confidence interval (95%CI) was calculated to express the association strength of AS. The linkage disequilibrium of IL-18 polymorphisms was investigated in AS occurrence by Haploview.Results: TT genotype and T allele of rs1946518 showed the significantly higher frequencies in AS patients than that of the controls (P=0.042, 0.026), which indicated that rs1946518 was obviously associated with AS risk (TT vs. GG: OR=1.993, 95%CI=1.021-3.891; T vs.G: OR=1.460, 95%CI=1.046-2.038). However, no significant association was found between rs187238 and AS. Moreover, the strong linkage disequilibrium was found and haplotype rs1946518T-rs187238C was a susceptible factor of AS (OR=1.638, 95%CI=1.017-2.639).Conclusion: IL-18 rs1946518 polymorphism may contribute to the susceptibility of individuals to AS in Chinese population, but not another polymorphism rs187238. The interaction of the two polymorphisms should be considered in AS etiology.

show abstract

“…Differences between here presented data and previous observations may be explained with varying sensitivity between detection systems, since IL-1RA is usually considered a quite robust marker of inflammasome activation. In addition to IL-1β cleavage and activation, caspase-1 is also responsible for the activation of the pro-inflammatory cytokine IL-18 ( 14 , 15 ). IL-18 is expressed by monocytes, lymphocytes, eosinophils, mast cells, and others.…”

Section: Discussionmentioning

confidence: 99%

Serum Interleukin-6 and CCL11/Eotaxin May Be Suitable Biomarkers for the Diagnosis of Chronic Nonbacterial Osteomyelitis

et al. 2017

View full text Add to dashboard Cite

ObjectivesChronic recurrent multifocal osteomyelitis (CRMO), the most severe form of chronic nonbacterial osteomyelitis (CNO), is an autoinflammatory bone disorder. In the absence of diagnostic criteria or biomarkers, CNO/CRMO remains a diagnosis of exclusion. The aim of this study was to identify biomarkers for diagnosing multifocal disease (CRMO).Study designSera from 71 pediatric CRMO patients, 11 patients with osteoarticular infections, 62 patients with juvenile idiopathic arthritis (JIA), 7 patients with para-infectious or reactive arthritis, and 43 patients with acute leukemia or lymphoma, as well as 59 healthy individuals were collected. Multiplex analysis of 18 inflammation- and/or bone remodeling-associated serum proteins was performed. Statistical analysis included univariate ANOVA, discriminant analysis, univariate receiver operating characteristic (ROC) analysis, and logistic regression analyses.ResultsFor 14 of 18 blood serum proteins, significant differences were determined between CRMO patients, at least one alternative diagnosis, or healthy controls. Multi-component discriminant analysis delivered five biomarkers (IL-6, CCL11/eotaxin, CCL5/RANTES, collagen Iα, sIL-2R) for the diagnosis of CRMO. ROC analysis allowed further reduction to a core set of 2 biomarkers (CCL11/eotaxin, IL-6) that are sufficient to discern between CRMO, healthy controls, and alternative diagnoses.ConclusionSerum biomarkers CCL11/eotaxin and IL-6 differentiate between patients with CRMO, healthy controls, and alternative diagnoses (leukemia and lymphoma, osteoarticular infections, para-infectious arthritis, and JIA). Easily accessible biomarkers may aid in diagnosing CRMO. Further studies testing biomarkers in larger unrelated cohorts are warranted.

show abstract

Caspase-1 als Regulator der Autoinflammation bei rheumatischen Erkrankungen

Cited by 11 publications

References 124 publications

Expression of Caspase-1 in breast cancer tissues and its effects on cell proliferation, apoptosis and invasion

Expression of Caspase-1 in breast cancer tissues and its effects on cell proliferation, apoptosis and invasion

Interleukin-18 Polymorphisms Impose Considerable Impacts on Ankylosing Spondylitis Occurrence

Serum Interleukin-6 and CCL11/Eotaxin May Be Suitable Biomarkers for the Diagnosis of Chronic Nonbacterial Osteomyelitis

Contact Info

Product

Resources

About