Cases of Subclinical Mastitis in Dairy Cattle, Microbiological Quality and Residues of Antibiotic Cow’s Milk: A Case Study in Tanggamus District

Abstract

The aim of this study was to determine cases subclinical mastitis in dairy cattle, microbiological quality and residues of antibiotic cow's milk from dairy farm in Tanggamus District. This research was conducted in January 2020 in Tanggamus District, Lampung Province. The milk samples used in this study were individual samples from all lactating cows, as many as 19 samples from 2 districts, namely Gisting Sub District and Air Naningan Sub District.The tests carried out were the somatic cells count (SCC) using the Breed method and mastitis IPB-1 test, total plate count (TPC), Staphylococcus aureus, coliform and Escherichia coli, density, pH values and antibiotic residue testing using Screening test/Bioassay. The data obtained were analyzed descriptively. The results of the study on SCC showed that Gisting Sub District had higher SCC than Air Naningan Sub District, which was > 400,000 cells/ml (2,554,285 cells/ml) and was supported by the IPB-1 mastitis test, which was 33.33% the teats that were positive for mastitis subclinical. The test results on TPC and E. coli showed that Air Naningan Sub District had higher TPC and E. coli values than Gisting Sub District, which were 3.39 log cfu/ml and 0.8 log cfu/ml, respectively. Meanwhile, the test results for the presence of S. aureus and coliform bacteria showed that Gisting Sub District had higher levels of S. aureus and coliform bacteria than Air Naningan Sub District, which were 0.32 log cfu/ml and 2.36 log cfu/ml, respectively. The results of density and pH values in 2 sub-districts showed almost the same, namely 1.030 and 6.88. The results of the Bioassay screening test for antibiotic residues in cow's milk in Tanggamus District showed that there were no samples containing penicillin, tetracycline, aminoglycoside, and macrolide antibiotic residues. The conclusion of this study was that there were cases of subclinical mastitis in dairy farms in Gisting and Air Naningan Districts based on the presence of SCC in milk and no antibiotic residue was found in milk.