Casein kinase 1α decreases β-catenin levels at adherens junctions to facilitate wound closure in<i>Drosophila</i>larvae

Tsai, Chang Ru; Galko, Michael J.

doi:10.1242/dev.175133

Cited by 5 publications

(4 citation statements)

References 56 publications

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“…The lethality suppressor screen, while not perfect, was nonetheless quite fruitful at expanding our collection of known WC genes beyond the JNK and actin pathways ( Lesch et al 2010 ; Brock et al 2012 ). Other genes that scored positive in this screen (CK1α) were also found in an analysis of adherens junctions at larval wound sites ( Tsai and Galko 2019 ).…”

Section: Resultsmentioning

confidence: 79%

Pvr and distinct downstream signaling factors are required for hemocyte spreading and epidermal wound closure at Drosophila larval wound sites

Tsai

Wang

Jacobson

et al. 2021

G3 Genes|Genomes|Genetics

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Tissue injury is typically accompanied by inflammation. In Drosophila melanogaster larvae, wound-induced inflammation involves adhesive capture of hemocytes at the wound surface followed by hemocyte spreading to assume a flat, lamellar morphology. The factors that mediate this cell spreading at the wound site are not known. Here, we discover a role for the platelet-derived growth factor/vascular endothelial growth factor-related receptor (Pvr) and its ligand, Pvf1, in blood cell spreading at the wound site. Pvr and Pvf1 are required for spreading in vivo and in an in vitro spreading assay where spreading can be directly induced by Pvf1 application or by constitutive Pvr activation. In an effort to identify factors that act downstream of Pvr, we performed a genetic screen in which select candidates were tested to determine if they could suppress the lethality of Pvr overexpression in the larval epidermis. Some of the suppressors identified are required for epidermal wound closure (WC), another Pvr-mediated wound response, some are required for hemocyte spreading in vitro, and some are required for both. One of the downstream factors, Mask, is also required for efficient wound-induced hemocyte spreading in vivo. Our data reveal that Pvr signaling is required for wound responses in hemocytes (cell spreading) and defines distinct downstream signaling factors that are required for either epidermal WC or hemocyte spreading.

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Section: Resultsmentioning

confidence: 79%

Pvr and distinct downstream signaling factors are required for hemocyte spreading and epidermal wound closure at Drosophila larval wound sites

Tsai

Wang

Jacobson

et al. 2021

G3 Genes|Genomes|Genetics

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“…Consistent with this possibility, ectopic expression of either CKIɑ or CKIIβ could not restore the proliferation defects observed in the absence of Yun (Figure S6). CKI and CKII are members of Ser/Thr kinases; they interact with many other proteins, such as β‐catenin, other kinases like PIP4K and S6KII, the RNA‐binding protein Orb, circadian proteins(Tim (Timeless) and Per (Period)), and are involved in multiple signalling pathways, including Wnt, Hh, and Hpo 36–41 . The two casein kinases are essential for ISC proliferation.…”

Section: Discussionmentioning

confidence: 99%

“…CKI and CKII are members of Ser/Thr kinases; they interact with many other proteins, such as β-catenin, other kinases like PIP4K and S6KII, the RNA-binding protein Orb, circadian proteins(Tim (Timeless) and Per (Period)), and are involved in multiple signalling pathways, including Wnt, Hh, and Hpo. [36][37][38][39][40][41] The two casein kinases are essential for ISC proliferation.…”

Section: Protein Phosphorylation Is One Of the Most Widely Observedmentioning

confidence: 99%

Phosphorylation of Yun is required for stem cell proliferation and tumorigenesis

et al. 2022

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Stem cells maintain adult tissue homeostasis under physiological conditions.Uncontrolled stem cell proliferation will lead to tumorigenesis. How stem cell proliferation is precisely controlled is still not fully understood. Phosphorylation of Yun is essential for ISC proliferation. Yun is essential for the proliferation of normal and transformed intestinal stem cells. Our mass spectrometry and biochemical data suggest that Yun can be phosphorylated at multiple residues in vivo. Interestingly, we show that the phosphorylation among these residues is likely interdependent. Furthermore, phosphorylation of each residue in Yun is important for its function in ISC proliferation regulation. Thus, our study unveils the important role of post-translational modification of Yun in stem cell proliferation.

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“…The lethality suppressor screen, while not perfect, was nonetheless quite fruitful at expanding our collection of known WC genes beyond the JNK and actin pathways (Brock et al 2012;Lesch et al 2010). Other genes that scored positive in this screen (CK1) were also found in an analysis of adherens junctions at larval wound sites (Tsai and Galko 2019).…”

Section: New Wound Closure Genes-ras Mkk3 and Maskmentioning

confidence: 99%

Pvr and downstream signaling factors are required for spreading of Drosophila hemocytes at larval wound sites

Tsai

Jacobson

et al. 2021

Preprint

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Tissue injury is typically accompanied by inflammation. In Drosophila melanogaster, wound-induced inflammation involves adhesive capture of hemocytes at the wound surface followed by hemocyte spreading to assume a flat, lamellar morphology. The factors that mediate this cell spreading at the wound site are not known. Here, we discover a role for the Platelet-derived growth factor (PDGF)/ Vascular endothelial growth factor (VEGF)-related receptor (Pvr) and its ligand, Pvf1, in blood cell spreading at the wound site. Pvr and Pvf1 are required for spreading in vivo and in an in vitro spreading assay where spreading can be directly induced by Pvf1 application or by constitutive Pvr activation. In an effort to identify factors that act downstream of Pvr, we performed a genetic screen in which select candidates were tested to determine if they could suppress the lethality of Pvr overexpression in the larval epidermis. Some of the suppressors identified are required for epidermal wound closure, another Pvr-mediated wound response, some are required for hemocyte spreading in vitro, and some are required for both. One of the downstream factors, Mask, is also required for efficient wound-induced hemocyte spreading in vivo. Our data reveals that Pvr signaling is required for wound responses in hemocytes (cell spreading) and defines distinct downstream signaling factors that are required for either epidermal wound closure or hemocyte spreading.

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Casein kinase 1α decreases β-catenin levels at adherens junctions to facilitate wound closure inDrosophilalarvae

Cited by 5 publications

References 56 publications

Pvr and distinct downstream signaling factors are required for hemocyte spreading and epidermal wound closure at Drosophila larval wound sites

Pvr and distinct downstream signaling factors are required for hemocyte spreading and epidermal wound closure at Drosophila larval wound sites

Phosphorylation of Yun is required for stem cell proliferation and tumorigenesis

Pvr and downstream signaling factors are required for spreading of Drosophila hemocytes at larval wound sites

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