Casein hydrolysates enhance osteoblast proliferation and differentiation in mouse bone marrow culture

Identification of buffalo casein-derived bioactive peptides with osteoblast proliferation activity

“…At lower concentration, PT hydrolysates did not show any proliferation effect, but it started inducing proliferation of calvarial cells at 50 µg/ ml, and the proliferation effect remained constant up to 100 µg/ml. Likewise, in our previous studies, Behera et al [20] reported that tryptic hydrolysates of buffalo casein showed significant difference in the proliferation of mouse bone marrow-derived osteoblast cells at 50 µg/ml. Xu [32] reported that the inclusion of whey protein concentrate at 20 µg/ml of the medium showed significant difference in the proliferation of rat calvarial cells (p < 0.05).…”

“…Earlier work also reported that L. helveticus-fermented milk prevented bone loss by decreasing bone turnover and increasing the bone mineral density in ovariectomized rats [19]. In vitro osteogenic potential of casein hydrolysates has been studied [20] previously in our laboratory, but the active component has not been delineated. Hence, in the present investigation, attempts have been made to study the effect of pepsin-trypsin hydrolysates of buffalo casein and four novel bioactive peptides of below 1 kDa derived from PT hydrolysates to induce osteoblast proliferation.…”

Comparisons of Processing Stability and Antioxidant Activity of the Silkworm Pupae Protein Hydrolysates by Spray-dry and Freeze-dry

“…Bioactive peptides from food-derived protein hydrolysates have been found to have positive effects on the major systems of the body such as the digestive, cardiovascular, nervous and immune systems [1,2]. Bioactive peptides have also been found to possess antioxidant capacity and their biological activity has been extensively studied [3][4][5].…”

Milk-Derived Bioactive Peptides With Antiosteoporotic Effect: A Mini Review

“…Over the last two decades, epidemiologic and intervention studies have shown that milk and milk-derived peptides prevented bone loss in pre-and postmenopausal women (Chee et al, 2003). Also, several studies thereafter have reported that milk derived bioactive peptides can induce osteoblast cells differentiation, maturation and matrix mineralization in vitro and thus could have beneficial effect against postmenopausal osteoporosis (Mada et al, 2018;Behera et al, 2013;Huttunen et al, 2008). In addition, studies have reported a positive association between high dietary protein intakes with bone mineral density and fracture repair (Wengreen et al, 2004), bone strength and bone formation (Huttunen et al, 2007).…”