Casein Haplotype Structure in Five Italian Goat Breeds

Sacchi, Paola; Chessa, S.; Budelli, E.; Bolla, P.; Ceriotti, G.; Soglia, Dominga; Rasero, Roberto; Cauvin, Elsa; Caroli, Anna

doi:10.3168/jds.s0022-0302(05)72825-3

Cited by 93 publications

(127 citation statements)

References 37 publications

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“…The positive value of F ST indicates that 5.16% of the genetic variation is explained by the difference between the caprine populations. These values, despite being based on only one locus data (Nei, 1987), agree with the result of study by Sacchi et al (2005) (F ST = 6.2%) in 5 caprine breeds using four loci (αs1-casein, αs2-casein, β-casein, and κ-casein). However, Saitbekova et al (1999) using microsatellites found a superior value in all loci for the F ST index of 17%.…”

Section: Population Structure and Divergencesupporting

confidence: 88%

“…In this study, the average heterozygote excess value inside each population was -0.0034, which is different from the positive values found in purebred goat populations from Italy, Morocco, France, China and Switzerland (Saitbekova et al 1999;Yang et al 1999;Tadlaoui et al 2002;Sacchi et al 2005). The positive value of F ST indicates that 5.16% of the genetic variation is explained by the difference between the caprine populations.…”

Section: Population Structure and Divergencecontrasting

confidence: 85%

“…The alleles of the CSN1S1 locus in goats have been grouped in 4 levels according to their individual approximate contribution to quantity of protein in milk; strong alleles (A, B 1 , B 2 , B 3 , B 4 , C, H, L and M), intermediate alleles (E and I), weak alleles (D, F and G), and null alleles (O 1 , O 2 and N) (Grosclaude et al 1987;Moioli et al 1998;Rando et al 2000;Bevilacqua et al 2002;Ramunno et al 2005;Sacchi et al 2005;Sztankóová et al 2007). In dairy goat populations, it is often considered convenient to identify the CSN1S1 genotypes of individual animals, with the idea of incorporating this information to the selection processes, and therefore propitiate an increase on the rate of genetic gain for casein contents and yield through gene assisted selection (GAS), compared to selection programs based only on phenotypic and pedigree information (BLUP), (Grosclaude et al 1987;Moioli et al 1998;Dekkers, 2004;Sánchez et al 2005).…”

mentioning

confidence: 99%

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Genetic polymorphism of the as1-casein locus in five populations of goats from Mexico

Torres‐Vázquez¹,

Flores²,

Montaldo³

et al. 2008

Electron. J. Biotechnol.

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Section: Population Structure and Divergencesupporting

confidence: 88%

Section: Population Structure and Divergencecontrasting

confidence: 85%

mentioning

confidence: 99%

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Genetic polymorphism of the as1-casein locus in five populations of goats from Mexico

Torres‐Vázquez¹,

Flores²,

Montaldo³

et al. 2008

Electron. J. Biotechnol.

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“…It has been established that the A, B and C alleles at CSN1S1 locus associated with the production of a high level of protein in milk, the E allele associated with a medium-level protein and F and G alleles with a low-level protein (Grosclaude et al, 1987 and. The distribution of different alleles at CSN1S1 locus has been investigated in European countries at the genomic as well as protein levels , Ramunno et al, 1991Bevilacqua et al, 2002;Sacchi et al, 2005). However, casein variability has not been well characterised in Indian goats except for the reports of Prakash et al (2002) and Rout et al (2004a).…”

Section: Introductionmentioning

confidence: 99%

Identification of the CSN1S1 allele in Indian goats by the PCR-RFLP method

Kumar

Rout

Mandal

et al. 2007

Animal

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The allelic distributions of the CSN1S1 (as 1 -casein) in the Indian goats are quite different from European goat breeds. Majority of Indian goat breeds and non-descript goats carry A, B, E and F alleles at as 1 -casein locus, as found by analysing both DNA and protein levels. However, A and B alleles, known to be associated with better casein yield, were observed in the highest proportion in all the Indian goat breeds. Gene frequency and breed heterozygosity were computed for the CSN1S1 gene. The gene frequency of allele A in Indian goats varies from 0.68 to 1.00 and allele B varies from 0.098 to 0.23. Allele F was observed in Beetal, Marwari, Chegu and non-descript goats of MP (Local MP) in less than 1% of population. The expected heterozygosity (He) varied from 0.141 to 0.506 over the population. The Beetal breed showed the highest gene diversity (0.506) followed by Jamunapari (0.395), Chegu (0.383) and Jakhrana (0.381) breeds. Therefore, the variability at CSN1S1 locus can be utilised for conservation as well as for genetic improvement of Indian goat breeds for increasing both the quality and quantity of milk production.

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“…It has been shown that goat casein variants differ among breeds and that they are related to region [6,7,20,22,39,44,47].…”

Section: Introductionmentioning

confidence: 99%

Protein composition and polymorphism in the milk of Skopelos goats

Moatsou

Vamvakaki

Mollé

et al. 2006

Lait

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-Individual milk samples taken from goats of the Skopelos breed of Greece were analyzed by RP-HPLC using as standards the casein fraction of milks of known genotypes. Milk samples with particular characteristics with respect to the casein fraction were further analyzed by IEF and RP-HPLC/ESI-MS. The mean total protein content (36.7 ± 2.6 g·L -1 ) and the mean casein content (29.7 ± 2.3 g·L -1 ) were high. The same was true for the α s1 -cn fraction, which was 21.8% of the total cn fraction, whereas the β-cn percentage of total cn was 43.8%. The respective values for α s2 -cn and κ-cn were 13.7% and 13.8%. These quantitative characteristics were consistent with the predominance of the strong α s1 -cn variants B3, B4 and As/B1. In all samples analyzed by RP-HPLC/ ESI-MS the deleted form differing from the respective complete form by Gln78 was found at different levels of phosphorylation, with the exception of variants F and D/G. The most frequent α s2 -cn variant was by far variant A followed by variants C and F, whereas compositional data implied the existence of a null allele. The detected β-cn forms were variants A and C in similar frequencies. Variant D (formerly B) of κ-cn predominated and the rare variant G was found. Different phosphorylation types of each casein variant and the characteristics of whey proteins were also determined.goat milk protein / casein genotype / phosphorylation / protein quantification / polymorphism Résumé -Polymorphisme des protéines du lait de chèvre de la race Skopelos. Des laits individuels de chèvres de Skopelos (Grèce) ont été analysés par RP-HPLC et les profils protéiques ont été comparés avec ceux issus de lait de chèvres de génotypes connus au locus α s1 . Les échantillons qui présentaient des caractéristiques particulières de leur fraction caséique ont été analysés par IEF et RP-HPLC/ESI-MS. Les teneurs moyennes en protéines totales (36,7 ± 2,6 g·L -1 ), et en caséines (29,7 ± 2,3 g·L -1 ), étaient plus élevées que dans les laits des races hautement sélectionnées. Le pourcentage en α s1 -cn représentait 21,8 % des caséines totales, tandis que celui de la β-cn atteignait 43,8 %. Les pourcentages en α s2 -cn et κ-cn étaient respectivement 13,7 % et 13,8 %. Ces caracté-ristiques quantitatives étaient corrélées avec la prédominance des variants forts B3, B4 et As/B1 de la α s1 -cn. Dans tous les échantillons analysés par RP-HPLC/ESI-MS, la forme déplétée en Gln78 de la α s1 -cn présentait plusieurs niveaux de phosphorylation, à l'exception des variants F et D/G. Le variant le plus abondant de la α s2 -cn était le variant A suivi par les variants C et F. Les variants A et C de la β-cn ont été trouvés en fréquences similaires. Le variant D (antérieurement dénommé B) de la κ-cn était prédominant bien que le variant rare G était aussi trouvé. Plusieurs niveaux de phosphorylation de chacun de ces variants ont été aussi mis en évidence, ainsi que les principales caractéristiques des protéines du sérum.polymorphisme / variant proteique / lait de chèvre / caséine / génotype /...

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Casein Haplotype Structure in Five Italian Goat Breeds

Cited by 93 publications

References 37 publications

Genetic polymorphism of the as1-casein locus in five populations of goats from Mexico

Genetic polymorphism of the as1-casein locus in five populations of goats from Mexico

Identification of the CSN1S1 allele in Indian goats by the PCR-RFLP method

Protein composition and polymorphism in the milk of Skopelos goats

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