Casein-derived antioxidative peptide prevents oxidative stress-induced dysfunction in osteoblast cells

Mada, Sanusi Bello; Reddi, Srinu; Kumar, Naveen; Vij, Rishika; Yadav, Radha; Kapila, Suman; Kapila, Rajeev

doi:10.1016/j.phanu.2018.07.001

Cited by 16 publications

(14 citation statements)

References 53 publications

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“…The osteoanabolic role of bioactive peptides cannot be ruled out in the detected effect of CETH. According to Amso et al [84] and Mada et al [85], individual short food peptides can reduce the expression of inflammatory and resorbing cytokines and stimulate differentiation of osteoblasts by increasing the expression of osteogenic genes and the activity of antioxidant enzymes.…”

Section: Discussionmentioning

confidence: 99%

Anti-Arthritic Effect of Chicken Embryo Tissue Hydrolyzate Against Adjuvant Arthritis in Rats (X-Ray Microtomographic and Histopathological Analysis)

Rzhepakovsky¹,

Avanesyan²,

Benlidayı³

et al. 2020

Preprint

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Finding new, safe strategies to prevent and control rheumatoid arthritis is an urgent task. Of particular interest in this regard are bioactive peptides and peptide-rich protein hydrolyzates, which represent a new trend in the development of functional foods and nutraceuticals. The resulting tissue hydrolyzate of the chicken embryo (CETH) has been evaluated for acute toxicity and tested against chronic arthritis induced by Freund's full adjuvant in rats. The anti-arthritic effect of CETH was studied on the 28th day of the experiment after two weeks of oral administration of CETH at doses of 60 and 120 mg/kg body weight. Arthritis was evaluated on the last day of the experiment on the injected animal paw using X-ray computerized microtomography and histopathology analysis methods. The CETH effect was compared with the non-steroidal anti-inflammatory drug diclofenac sodium (5 mg/kg). Oral administration of CETH was accompanied by effective dose-dependent correction of morphological changes caused by the adjuvant injection. CETH had relatively high recovery effects in terms of parameters for reducing inflammatory edema, inhibition of osteolysis, prevention of osteophitosis, reduction of the inflammatory reaction of periarticular tissues, and cartilage degeneration. This study presents a potential theoretical strategy for the safe correction of this pathological process and, for the first time, shows that CETH may be a powerful potential nutraceutical agent or bioactive component of functional products in the treatment of rheumatoid arthritis.

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Section: Discussionmentioning

confidence: 99%

Anti-Arthritic Effect of Chicken Embryo Tissue Hydrolyzate Against Adjuvant Arthritis in Rats (X-Ray Microtomographic and Histopathological Analysis)

Rzhepakovsky¹,

Avanesyan²,

Benlidayı³

et al. 2020

Preprint

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“…Intracellular ROS content was evaluated by quantifying the fluorescence emitted by 2' ,7'-dichlorofluorescein, the oxidized form of 2' ,7'-dichlorofluorescein diacetate (DCFH-DA) according to the instruction of a ROS assay kit. The fluorescent intensity was measured at excitation wavelength 485 nm and emission wavelength 530 nm using a multi-mode detection plate reader (Mada et al, 2018).…”

Section: Determination Of Sod Cat Mda and Ros Levelsmentioning

confidence: 99%

“…After washing with distilled water, the cells were stained with 40 mM Alizarin Red S for 30 min. To quantify the bound dye, the stain was solubilized with 10% cetylpyridinium chloride in the dark for 1 h. The absorbance of the solubilized stain was measured at 570 nm using a microplate reader (Mada et al, 2018).…”

Section: Mineralization Assaymentioning

confidence: 99%

“…Bioactive peptides produced by enzymatic hydrolysis of food proteins have been shown to possess osteoprotective and antioxidant activities. For example, casein-derived peptide has been reported to ameliorate oxidative stress-induced osteoblasts dysfunction and increase cell viability, superoxide dismutase (SOD) and catalase (CAT) activities (Mada et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Ameliorative effects of phosphorylated peptide from Antarctic krill (Euphausia superba) against H2O2-induced damage in MC3T3-E1 cells

2022

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Phosphorylated peptide from Antarctic krill (P-AKP) was prepared by the dry-heating method with sodium pyrophosphate in order to improve its antioxidant activity and osteogenic activity. P-AKP exhibited more competitive DPPH• and OH• scavenging activities compared to the native Antarctic krill peptide (AKP). In hydrogen peroxide (H 2 O 2 )-induced oxidative damage of MC3T3-E1 cells, both AKP and P-AKP pretreatment could dose-dependently improve superoxide dismutase (SOD) and catalase (CAT) activities through attenuating the accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA) production. Moreover, AKP and P-AKP prevented oxidative stress-induced down regulation of alkaline phosphatase (ALP) activity and matrix mineralization. Particularly, the promoting effects of P-AKP on the enzymatic antioxidant defense system, differentiation and mineralization was higher than that of AKP. These results suggested that phosphorylation might be a promising approach to improve the antioxidant and osteogenic activity of AKP, and P-AKP could be a beneficial agent for attenuating oxidative stress-related bone loss.

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“…Over the last two decades, epidemiologic and intervention studies have shown that milk and milk-derived peptides prevented bone loss in pre-and postmenopausal women (Chee et al, 2003). Also, several studies thereafter have reported that milk derived bioactive peptides can induce osteoblast cells differentiation, maturation and matrix mineralization in vitro and thus could have beneficial effect against postmenopausal osteoporosis (Mada et al, 2018;Behera et al, 2013;Huttunen et al, 2008). In addition, studies have reported a positive association between high dietary protein intakes with bone mineral density and fracture repair (Wengreen et al, 2004), bone strength and bone formation (Huttunen et al, 2007).…”

Section: Milk-derived Peptides With Antiosteoporotic Effects In Vitromentioning

confidence: 99%

Milk-Derived Bioactive Peptides With Antiosteoporotic Effect: A Mini Review

Mada

Abaya²,

James

et al. 2020

FJS

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Postmenopausal osteoporosis is a global health problem characterized by decreased in bone mineral density (BMD) and progressive deterioration of microarchitecture and subsequent increase in bone fragility and susceptibility to fracture. More than 200 million people suffer from osteoporosis worldwide with about 8.9 million fractures and the prevalence rate of osteoporosis is expected to increase significantly in the future because of increased in life expectancy and aging population. Milk-derived bioactive peptides from cow, goat, sheep, buffalo, and camel exhibit several potential health promoting effect including antiosteoporosis, antihypertensive, antioxidative, antithrombotic, immunomodulatory and anti-inflammatory effects. Epidemiological and intervention studies have shown that milk and milk-derived peptides prevented bone loss in pre- and postmenopausal women. Moreover, quite a lot of studies have reported that milk-derived bioactive peptides can induce osteoblast cell proliferation, differentiation and also prevented bone loss in osteoporotic rats model. Thus, milk-derived peptides exhibits beneficial effect against bone-related diseases and can be of particular interest towards prevention and management of postmenopausal osteoporosis. Hence, the present review summarizes various studies using ISI, SCOPUS and PubMed indexed journals to elucidate the potential role of milk-derived bioactive peptides with in vitro and in vivo antiosteoporotic property

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Casein-derived antioxidative peptide prevents oxidative stress-induced dysfunction in osteoblast cells

Cited by 16 publications

References 53 publications

Anti-Arthritic Effect of Chicken Embryo Tissue Hydrolyzate Against Adjuvant Arthritis in Rats (X-Ray Microtomographic and Histopathological Analysis)

Anti-Arthritic Effect of Chicken Embryo Tissue Hydrolyzate Against Adjuvant Arthritis in Rats (X-Ray Microtomographic and Histopathological Analysis)

Ameliorative effects of phosphorylated peptide from Antarctic krill (Euphausia superba) against H2O2-induced damage in MC3T3-E1 cells

Milk-Derived Bioactive Peptides With Antiosteoporotic Effect: A Mini Review

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