Case series: The use of a dehydrated human amnion/chorion membrane allograft to enhance healing in the repair of lower eyelid defects after Mohs micrographic surgery

Wisco, Oliver J.

doi:10.1016/j.jdcr.2016.06.002

Cited by 14 publications

(9 citation statements)

References 12 publications

(12 reference statements)

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“…We optimized a trypsin-EDTA, nuclease decellularization protocol that eliminated cell and nuclear materials from the amnion. While, other groups have used their amnion material for body surface repair like foot ulceration repair [36], [37], lower eyelid repair [16], and fistula reconstruction [15], we have focused on an internal cardiovascular application for our developed amnion based bioscaffold.…”

Section: Discussionmentioning

confidence: 99%

“…It has been tested in animal models for urethra repair, ocular surface reconstruction, liver repair, and pericardial closure [10]- [13]. In human patients, it has been used for lower eyelid repair, fistula closures, and neovagina construction [14]- [16]. Additionally, it has been shown that amnion has anti-inflammatory and anti-microbial properties [17]- [21].…”

Section: Introductionmentioning

confidence: 99%

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Amnion-Based Scaffold with Enhanced Strength and Biocompatibility for In Vivo Vascular Repair

Swim

Albertario

Iacobazzi

et al. 2019

Tissue Engineering Part A

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Current vascular replacement grafts used in congenital heart defect corrective surgery have poor longevity and growth potential. Recipient patients often require multiple reoperations. Tissue engineering has the promise to produce a graft with the potential to grow, remodel and repair. Here we aimed at developing an amnion-based scaffold suitable for cardiovascular tissue engineering applications and in vivo usage. The developed human amnion-based scaffold was made by an enzymatic decellularization process followed by freeze-drying as a single or multi-layered structure. These structures were compared to native amnion for seeded cell viability and biomechanical properties then tested for in vivo biocompatibility. Our results demonstrated that while native amnion tissue supported little cell growth, the decellularized-amnion allowed cell engraftment and proliferation cell survival. Additionally, preservation of the scaffold by freeze-drying as a single layer, allowed further improved cell engraftment and cell growth. Multi-layering the freeze-dried amnion-scaffolds resulted in a similar cell growth potential of the single layered construct but superior mechanical strength. The multi-layered construct showed in vitro biocompatibility with endothelial cells, smooth muscle cells, cardiac myocytes, and thymus and cord-blood-derived MSCs. When implanted in a piglet model of left pulmonary artery grafting, the multi-layered construct showed its in vivo suitability and biocompatibility for vascular repair as demonstrated by the development of newly formed endothelium in the intima, a smooth muscle cell-rich medial layer and an adventitia containing new vasa vasorum. endothelial cell layer in the inner side of the graft and a smooth muscle layer in the outer side. In conclusion, our developed amnion-derived scaffold represents an off-the-shelf biocompatible structure that can be seeded with the patient's own MSCs to produce an autologous vascular graft.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Amnion-Based Scaffold with Enhanced Strength and Biocompatibility for In Vivo Vascular Repair

Swim

Albertario

Iacobazzi

et al. 2019

Tissue Engineering Part A

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“…The cells derived from human amniotic membranes (hAM) are suitable for regenerative medicine because they are associated with low immunogenicity and possess anti-inflammatory, antifibrotic, and other clinically relevant properties [ 37 , 38 ]. Dehydrated human amnion/chorion membrane (DHCM) allografts significantly enhance wound healing and rectify full-thickness scalp and lower eyelid defects after Mohs micrographic surgery [ 39 , 40 ]. Murphy et al showed that amnion membrane-derived hydrogel and amnion membrane powder significantly enhanced the formation of a mature epidermis in the mouse wound healing model and a full-thickness porcine skin wound healing model [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

LOXL2 from human amniotic mesenchymal stem cells accelerates wound epithelialization by promoting differentiation and migration of keratinocytes

Zhao

Jiang

et al. 2020

Aging

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In this study, we identified wound healing-related proteins secreted by human amniotic epithelial cells (hAECs) and human amniotic mesenchymal stem cells (hAMSCs). We observed increased migration and reduced proliferation and differentiation when keratinocytes were co-cultured in media conditioned by hAECs (hAECs-CM) and hAMSCs (hAMSCs-CM). Label-free mass spectrometry and bioinformatic analyses of the hAECs-CM and hAMSCs-CM proteome revealed several proteins associated with wound healing, angiogenesis, cellular differentiation, immune response and cell motility. The levels of the proteins related to wound healing, including CTHRC1, LOXL2 and LGALS1, were significantly higher in hAMSCs-CM than hAECs-CM. LOXL2 significantly enhanced in vitro keratinocyte migration and differentiation compared to CTHRC1 and LGALS1. Moreover, LOXL2 enhanced keratinocyte migration and differentiation by activating the JNK signaling pathway. We observed significant reduction in the in vitro migration and differentiation of keratinocytes when co-cultured with medium conditioned by LOXL2-silenced hAMSCs and when treated with 10 μM SP600125, a specific JNK inhibitor. Treatment with hAMSCs-CM and LOXL2 significantly accelerated wound healing in the murine skin wound model. These findings show that LOXL2 promotes wound healing by inducing keratinocyte migration and differentiation via a JNK signaling pathway.

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“…Various grafts have been used, including cryopreserved, ultra-thick, fresh, and dehydrated amniotic membrane. 13,[57][58][59][60][61][62][63][64] Mainly, case reports and case series have been published. Patient populations have included those with conjunctival melanoma with eyelid involvement, orbital sebaceous carcinoma involving the lower eyelid, squamous cell and basal cell carcinoma, and superficial lower eyelid defects after Mohs surgery.…”

Section: Reconstruction After Palpebral or Forniceal Conjunctival Les...mentioning

confidence: 99%

A Review of Current Uses of Amniotic Membrane Transplantation in Ophthalmic Plastic and Reconstructive Surgery

Parikh,

Conger,

et al. 2023

Ophthalmic Plastic &Amp; Reconstructive Surgery

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Purpose: To review and summarize the existing literature on the clinical applications of amniotic membrane transplantation (AMT) in ophthalmic plastic and reconstructive surgery. Methods: A literature review was conducted on the PubMed database using the following search terms: “amniotic membrane” and “eyelid” or “orbit” or “fornix” or “socket” or “lacrimal”. Results: In total 516 articles resulted from the search, of which 62 were included. Numerous cases and case series have been published on the use of amniotic membrane transplantation for ocular surface reconstruction, eyelid and forniceal reconstruction, and cicatricial eyelid abnormalities. Surgical methods of securing the graft vary. Few comparative studies exist; some show a similar or improved result when compared to oral mucous membrane grafting for certain indications. Conclusions: Amniotic membrane transplantation can be a useful tool for the oculoplastic surgeon when faced with a case requiring reconstruction of the posterior lamellae, particularly in patients without other graft donor sites available, and uses of AMT continue to expand. Additional studies directly comparing AMT to other reconstructive techniques would be helpful in choosing between the available surgical techniques and standardizing best practices.

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Case series: The use of a dehydrated human amnion/chorion membrane allograft to enhance healing in the repair of lower eyelid defects after Mohs micrographic surgery

Cited by 14 publications

References 12 publications

Amnion-Based Scaffold with Enhanced Strength and Biocompatibility for In Vivo Vascular Repair

Amnion-Based Scaffold with Enhanced Strength and Biocompatibility for In Vivo Vascular Repair

LOXL2 from human amniotic mesenchymal stem cells accelerates wound epithelialization by promoting differentiation and migration of keratinocytes

A Review of Current Uses of Amniotic Membrane Transplantation in Ophthalmic Plastic and Reconstructive Surgery

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