Cascading and precise regulation of the selective bio-production of 2- or 5-ketogluconic acid from glucose with whole-cell catalysis technology

Hua, Xia; Han, Jun; Liu, XinLu; Xu, Yong

doi:10.1039/d2gc04566d

“…The xylose and XA concentrations were quantified by high-performance anion-exchange chromatography (HPAEC) equipped with a pulsed amperometric detector (Thermo ICS-5000). Detection conditions were as follows: The chromatographic column was CarboPac™ PA10, the mobile phase was 100 mM NaOH, the flow rate was 0.3 mL/min, and the detection time was 30 min 34 .…”

Section: Methodsmentioning

confidence: 99%

Threshold effect on whole-cell catalysis of extra hyperviscous biosystem by a sealed oxygen supply biotechnology

XU,

Hua,

Hu

et al. 2024

Preprint

0

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The end-product concentration and productivity are critical issues in economic competition between biotechnological commercials and the chemical engineering industry. The prominent contradiction between high-titer products and the large-scale oxygen demand for aerobic biocatalysis leads to hyperviscosity, mass transport bottleneck in [dynamically changing](javascript:;) polyphase biosystems, and severe foaming problems. In this study, an intensification strategy for the whole-cell catalytic preparation of high-titer xylonic acid by Gluconobacter oxydans in a sealed-compressed oxygen supply bioreactor is propose. Multi-scale control factors are quantitatively studied to determine the biochemical parameter thresholds, and theoretically calculated the optimal production performance based on threshold effect. Finally, 650.8 g/L xylonic acid is obtained with a maximum productivity of 41.7 g/L/h with a catalytic performance of 95.8%, compared with the theoretical calculations. The intensification strategy for the oxygen transfer threshold effect overcome the stubborn obstacles of obligate aerobic catalysis, while providing a sustainable value-added pathway for fermentative lignocellulose.

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“…The xylose and XA concentrations were quantified by high-performance anion-exchange chromatography (HPAEC) equipped with a pulsed amperometric detector (Thermo ICS-5000). Detection conditions were as follows: The chromatographic column was CarboPac™ PA10, the mobile phase was 100 mM NaOH, the flow rate was 0.3 mL/min, and the detection time was 30 min 33 .…”

Section: Methodsmentioning

confidence: 99%

Threshold effect on whole-cell catalysis of extra hyperviscous biosystem by a sealed oxygen supply biotechnology

Hua,

Hu,

Han

et al. 2024

Preprint

0

View full text Add to dashboard Cite

The end-product concentration and productivity are critical issues in economic competition between biotechnological commercials and the chemical engineering industry. The prominent contradiction between high-titer products and the large-scale oxygen demand for aerobic biocatalysis leads to hyperviscosity, mass transport bottleneck in [dynamically changing](javascript:;) polyphase biosystems, and severe foaming problems. In this study, an intensification strategy for the whole-cell catalytic preparation of high-titer xylonic acid by Gluconobacter oxydans in a sealed-compressed oxygen supply bioreactor is propose. Multi-scale control factors are quantitatively studied to determine the biochemical parameter thresholds, and theoretically calculated the optimal production performance based on threshold effect. Finally, 650.8 g/L xylonic acid is obtained with a maximum productivity of 41.7 g/L/h with a catalytic performance of 95.8%, compared with the theoretical calculations. The intensification strategy for the oxygen transfer threshold effect overcome the stubborn obstacles of obligate aerobic catalysis, while providing a sustainable value-added pathway for fermentative lignocellulose.

show abstract

A high-performance anion-exchange chromatographic method for the fast analysis and precise determination of varied glucose-derived acids during biomass biorefinery

Hu,

Haq,

Xu

et al. 2024

Food Chemistry

0

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Cascading and precise regulation of the selective bio-production of 2- or 5-ketogluconic acid from glucose with whole-cell catalysis technology

Abstract: Ketogluconic acid is widely applied in several industries as the bio-precursor of vitamin C and tartaric acid, while the selective production of 2-ketogluconic acid (2-kGA) or 5-ketogluconic acid (5-kGA) from...

Cited by 4 publications

References 43 publications

Threshold effect on whole-cell catalysis of extra hyperviscous biosystem by a sealed oxygen supply biotechnology

Threshold effect on whole-cell catalysis of extra hyperviscous biosystem by a sealed oxygen supply biotechnology

Threshold effect on whole-cell catalysis of extra hyperviscous biosystem by a sealed oxygen supply biotechnology

A high-performance anion-exchange chromatographic method for the fast analysis and precise determination of varied glucose-derived acids during biomass biorefinery

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