Carvacrol suppresses LPS-induced pro-inflammatory activation in RAW 264.7 macrophages through ERK1/2 and NF-kB pathway

Somensi, Nauana; Rabelo, Thallita Kelly; Guimarães, Adriana Gibara; Quintans‐Júnior, Lucindo José; Araújo, Adriano Antunes de Souza; Moreira, José Cláudio Fonseca; Gelain, Daniel Pens

doi:10.1016/j.intimp.2019.105743

Cited by 83 publications

(54 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Carvacrol (5-isopropyl-2-methylphenol) ( Figure 3) is a phenolic monoterpene that has described pharmacological activities, including antioxidant, antibacterial [26], anti-inflammatory [25], cardioprotective [53] antinociceptive, and gastroprotective [54]. To assess the intestinal anti-inflammatory activity of carvacrol, rats were subjected to intrarectal administration of acetic acid (5%) to induce colitis.…”

Section: Carvacrolmentioning

confidence: 99%

See 1 more Smart Citation

Intestinal Anti-Inflammatory Activity of Terpenes in Experimental Models (2010–2020): A Review

et al. 2020

View full text Add to dashboard Cite

Inflammatory bowel diseases (IBDs) refer to a group of disorders characterized by inflammation in the mucosa of the gastrointestinal tract, which mainly comprises Crohn’s disease (CD) and ulcerative colitis (UC). IBDs are characterized by inflammation of the intestinal mucosa, are highly debilitating, and are without a definitive cure. Their pathogenesis has not yet been fully elucidated; however, it is assumed that genetic, immunological, and environmental factors are involved. People affected by IBDs have relapses, and therapeutic regimens are not always able to keep symptoms in remission over the long term. Natural products emerge as an alternative for the development of new drugs; bioactive compounds are promising in the treatment of several disorders, among them those that affect the gastrointestinal tract, due to their wide structural diversity and biological activities. This review compiles 12 terpenes with intestinal anti-inflammatory activity evaluated in animal models and in vitro studies. The therapeutic approach to IBDs using terpenes acts basically to prevent oxidative stress, combat dysbiosis, restore intestinal permeability, and improve the inflammation process in different signaling pathways.

show abstract

Section: Carvacrolmentioning

confidence: 99%

“…Several preclinical studies show evidence that terpenes have pharmacological activities such as anti-inflammatory [25], antioxidant [26], antibacterial [27], gastroprotective, and gastric healing [28].…”

Section: Introductionmentioning

confidence: 99%

Intestinal Anti-Inflammatory Activity of Terpenes in Experimental Models (2010–2020): A Review

et al. 2020

View full text Add to dashboard Cite

show abstract

“…It has been shown that LPS can activate NF‐κB signalling pathway and promote the production of downstream pro‐inflammatory mediators (He et al., 2017). Somensi N has found that carvacrol can significantly inhibit the activation of NF‐κB which induced by LPS and play an anti‐inflammatory role (Somensi et al., 2019). Yang C et al also found that shikonin could inhibit NF‐κB signalling pathway in the process of anti‐inflammatory (Yang et al., 2018).…”

Section: Discussionmentioning

confidence: 99%

Palmatine attenuates LPS‐induced inflammatory response in mouse mammary epithelial cells through inhibiting ERK1/2, P38 and Akt/NF‐кB signalling pathways

Zhang

Wang

et al. 2020

Animal Physiology Nutrition

View full text Add to dashboard Cite

Palmatine has a wide range of pharmacological effects and anti‐inflammatory function. However, the effect of palmatine on LPS‐induced inflammatory response of mammary epithelial cells has not been reported. In this research, we studied the anti‐inflammatory mechanism of palmatine in EpH4‐Ev (mouse mammary epithelial cells). EpH4‐Ev cells were pre‐treated with palmatine and then incubated with LPS. Cells were collected for examining production of pro‐inflammatory mediators by qRT‐PCR, and the related inflammatory signalling pathway was detected through immunofluorescence and Western blot. The results found that palmatine could significantly reduce the expression of IL‐6, TNF‐α, IL‐1β and COX‐2 in EpH4‐Ev cells. Research on mechanisms found that palmatine could significantly inhibit the protein levels of p‐Akt, p‐P65, p‐ERK1/2 and p‐P38 in EpH4‐Ev cells. In conclusion, these data suggested that palmatine inhibits inflammatory response in LPS‐induced EpH4‐Ev cells via down‐regulating Akt/ NF‐кB, ERK1/2 and P38 signalling pathways.

show abstract

“…RAW 264.7 cells (1 × 10 5 cells/well) were seeded in 96-well cell culture plates and allowed to adhere for 12 h. The cells were incubated on swertiamarin and loganic acid (0, 5, 10, 20, 40, 80, and 100 µM), or swertiamarin and vitexin (0, 5, 10, 20, 40, and 50 µM), respectively, with stimulation by LPS (1 µg/mL) for 24 h [34]. NO secretion by LPS-stimulated macrophages was determined by Griess reagents (Beyotime, Shanghai, China) according to the instructions of manufacturer [35]. Absorbance was measured at 540 nm and NO concentration was determined using sodium nitrite as a standard.…”

Section: Nitric Oxide (No) Assaymentioning

confidence: 99%

Traceability of Geographical Origin in Gentiana straminea by UPLC-Q Exactive Mass and Multivariate Analyses

et al. 2019

View full text Add to dashboard Cite

The root of Gentiana straminea Maxim. (Gentianaceae), is officially listed as “Qin-Jiao” in the Chinese Pharmacopoeia for the treatment of rheumatic arthritis, icteric hepatitis, constipation, pain, and hypertension. To establish the geographical origin traceability in G. straminea, its chemical profiles were determined by a UPLC-Q exactive mass spectrometer, from which 43 compounds were identified by comparing retention times and mass spectrometry. Meanwhile, a pair of isomers (loganin and secologanol) was identified by mass spectrometry based on their fragmentation pathway. A total of 42 samples from difference habitats were determined by an UPLC-Q exactive mass spectrometer and the data were assayed with multivariate statistical analysis. Eight characteristic compounds were identified to determine the geographical origin of the herb. To estimate the key characteristic markers associated with pharmacological function, the inhibiting activities of nitric oxide (NO) production in lipopolysaccharide (LPS)-induced macrophages were examined. This finding is crucial in realizing the determination of botanical origin and evaluating the quality of G. straminea.

show abstract

Carvacrol suppresses LPS-induced pro-inflammatory activation in RAW 264.7 macrophages through ERK1/2 and NF-kB pathway

Cited by 83 publications

References 44 publications

Intestinal Anti-Inflammatory Activity of Terpenes in Experimental Models (2010–2020): A Review

Intestinal Anti-Inflammatory Activity of Terpenes in Experimental Models (2010–2020): A Review

Palmatine attenuates LPS‐induced inflammatory response in mouse mammary epithelial cells through inhibiting ERK1/2, P38 and Akt/NF‐кB signalling pathways

Traceability of Geographical Origin in Gentiana straminea by UPLC-Q Exactive Mass and Multivariate Analyses

Contact Info

Product

Resources

About