Carum carvi mediated green synthesis of copper nanoparticles and its effect on Solanum lycopersicum seedlings

Abstract

The present study aims to synthesis the copper nanoparticles (CuNPs) and their effect on the biochemical and physiological characteristics of Solanum lycopersicum. The results exhibited the color change in the Carum carvi aqueous extract from clear deep brown to a greenish color and this observation indicated the green-biosynthesis of CuNPs by reduction of Cu+ to Cu0. Also, the absorbance broadening band for the green-biosynthetic CuNPs appeared at the 340 nm wavelength using UV-Vis but the C. carvi aqueous extract not showed any peaks at this wavelength. SEM analysis exhibited the micrographic surface morphology and the shape of the green-biosynthetic CuNPs with a scan area of 50 μm and showed the spherical shape particles of CuNPs aggregation. The three-dimensional image and the surface morphology of green-biosynthesized CuNPs and C. carvi aqueous extract were examined using AFM analysis that showed the surface of C. carvi aqueous extract was 45.5 nm size with non-homologus and irregular form of distribution, but the green-biosynthesized CuNPs were 12.4 nm size in nanoscale with regular and homogenous distribution form. The results also showed that the effect of bio-synthesized copper nanoparticles was evident on the S. lycopersicum seedlings fresh and dry weight according to the different reading times after treatment with nanoparticles. Also, the concentration of 2.5 mg/mL (CuNPs) showed a significant increase in the chlorophyll content (58.51 μg/cm²) on the 21st day after treatment and a significant increase in the activity of peroxidase enzyme (35.12 U min-1 mg-1 protein) was obtained at the concentration of 2.5 mg/mL (CuNPs) at 21st day after germination.

Biosynthesis of Copper Nanoparticles with Medicinal Plants Extracts: From Extraction Methods to Applications

“…in conjugation with (CuSO 4 )•5H 2 O, exhibiting a diameter of 6 nm without apparent aggregation [104]. A similar case was found in NPs obtained with extracts of Galeopsis herba and Carum carvi in conjugation with the same precursor salt Cu(NO 3 )2 •3H 2 O, where the synthesized NPs had a diameter of 5-10 and 12 nm, respectively [90,105]. However, the synthesis conditions of the three green synthesis protocols do not present any convergence in the applied variables.…”

“…CuSO 4 NPs significantly increased the shoot length, root length, and fresh weight over control of Verbena bipinnatifida seedlings [179]. Furthermore, the treatment of Solanum lycopersicum plantlets with green-biosynthetic CuNPs resulted in a substantial increase in their growth and in the increase of sugars and pigments, favoring the organoleptic properties of the fruit [105].…”

EFFECT OF CuO NANOPARTICLES ON SEED GERMINATION AND SEEDLING GROWTH IN ECHINACEA PURPUREA IN VITRO.

“…Nano particles approved to have a positive impact as anti-inflammatory and antibacterial effects (8). Copper nanoparticle absorption and accumulation are directly correlated with the concentration used (16) and are easily made in the lab as dark brownish-red powdered items (3,4,6,20). Although the practices of growing Echinacea plants from seeds was common, it has a number of drawbacks, such as the uncertainty that the plants produced will be free of diseases due to their lengthy growth cycles and the dormancy of Echinacea seeds (22,26).…”