Cartilage regeneration with highly-elastic three-dimensional scaffolds prepared from biodegradable poly(l-lactide-co-ɛ-caprolactone)

Jung, Youngmee; Park, Min Sung; Lee, Jin Woo; Kim, Young Ha; Kim, Sang Heon; Kim, Soo Hyun

doi:10.1016/j.biomaterials.2008.08.031

Cited by 102 publications

(78 citation statements)

References 25 publications

(24 reference statements)

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“…24 In an effort to overcome this limitation, rabbit cells were cultured in cell culture medium containing 2% rabbit serum. To ensure the MSCs retained their plasticity, tri-lineage differentiation assays 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 Regarding inflammation, the PLCL scaffold was biocompatible with no evidence of inflammation or giant cells in or around the struts of the template after 4 weeks implantation, which agrees well with previous studies by Jung et al, 15,16 where PLCL was also shown not to evoke an adverse inflammatory response in vivo. On first observation, it appeared that the tissue repair in the empty defect was better than that of the scaffolds.…”

Section: Discussionsupporting

confidence: 88%

“…Moreover, there was evidence of chondrocyte clustering and hypo-and hyper-cellularity in the repair and native tissues adjacent to the empty defect, which compared well with previous findings where fibrous tissue formation was observed in empty defects and is perhaps why empty defects, are accepted as a negative control . 2,10 Of more interest was the fact the functionally-graded pore structure appeared to go one step forward compared to Jung 15,16 and enhanced endogenous cell recruitment, integration and neotissue formation, with immature chondrocytes and collagen type II staining visible throughout and under the cell-free PLCL construct. This correlates well with other studies, where cartilage repair was attributed to cell homing, engraftment and repair due to pore architecture of the construct used.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the Early In Vivo Response of a Functionally Graded Macroporous Scaffold in an Osteochondral Defect in a Rabbit Model

et al. 2015

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Abstract:Cartilage tissue engineering is a multifactorial problem requiring a wide range of material property requirements from provision of biological cues to facilitation of mechanical support in load-bearing diarthrodial joints. The study aim was to design, fabricate and characterize a template to promote endogenous cell recruitment for enhanced cartilage repair. A polylactic acid poly-ε-caprolactone (PLCL) support structure was fabricated using laser micromachining technology and thermal crimping to create a functionally-graded open pore network scaffold with a compressive modulus of 10MPa and a compressive stress at 50% strain of 8.5MPa. In parallel, rabbit mesenchymal stem cells (MSC) were isolated and their growth characteristics, morphology and multipotency confirmed. Sterilization had no effect on construct chemical structure and cellular compatibility was confirmed. After four weeks implantation in an osteochondral defect in a rabbit model to assess biocompatibility, Powered by Editorial Manager® and ProduXion Manager® from Aries Systems Corporationthere was no evidence of inflammation or giant cells. Moreover, acellular constructs performed better than cell-seeded constructs with endogenous progenitor cells homing through microtunnels, differentiating to form neo-cartilage and strengthening integration with native tissue. These results suggest, albeit at an early stage of repair, that by modulating the architecture of a macroporous scaffold, pre-seeding with MSCs is not necessary for hyaline cartilage repair.Author Comments:Additional Information: Question ResponsePlease state the number of words in your manuscript including references. 5654 Powered by Editorial Manager® and ProduXion Manager® from Aries Systems Corporation 1Overall Review "This is an important study that demonstrates that an inherent advantage of the osteochondral approach when a macroporous biomaterial is used is that pre-seeding of cells is not necessary. This may be a valuable addition to the literature, and the authors are encouraged to modify the title and abstract to reflect this point of impact/significance in the field." Comment: The authors thank the reviewers for the positive feedback and have modified the title and the abstract to reflect their comments. Actions: Title:The new title of the manuscript is "Evaluation of the Early In vivo Response of a Functionally Graded Macroporous Scaffold in an Osteochondral Defect in a Rabbit Model" Abstract "Cartilage tissue engineering is a multifactorial problem requiring a wide range of material property requirements from provision of biological cues to facilitation of mechanical support in load-bearing diarthrodial joints. The study aim was to design, fabricate and characterize a template to promote endogenous cell recruitment for enhanced cartilage repair. A polylactic acid poly-ε-caprolactone (PLCL) support structure was fabricated using laser micromachining technology and thermal crimping to create a functionallygraded open pore network scaffold with a compressive modulus of 10MP...

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Evaluation of the Early In Vivo Response of a Functionally Graded Macroporous Scaffold in an Osteochondral Defect in a Rabbit Model

et al. 2015

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“…Biodegradable polymers such as poly(L-lactide-cocaprolactone) (PLCL, 5:5) (Jung et al, 2008), poly(3-hydroxy-butyrate-co-3-hydroxyhexanoate) (PHBHHx) (Wang et al, 2008), and poly(1,8-octanediol citrate) (POC) (Kang et al, 2006) have been utilized as scaffold materials for cartilage tissue engineering. One point in common of these materials was their complete rubber-like elasticity.…”

Section: Discussionmentioning

confidence: 99%

Microstructure and properties of nano-fibrous PCL-b-PLLA scaffolds for cartilage tissue engineering

Liu²,

Guan³

et al. 2009

eCM

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Nano-fibrous scaffolds which could potentially mimic the architecture of extracellular matrix (ECM) have been considered a good candidate matrix for cell delivery in tissue engineering applications. In the present study, a semicrystalline diblock copolymer, poly(ε-caprolactone)-block-poly(L-lactide) (PCL-b-PLLA), was synthesized and utilized to fabricate nano-fibrous scaffolds via a thermally induced phase separation process. Uniform nano-fibrous networks were created by quenching a PCL-b-PLLA/THF homogenous solution to -20ºC or below, followed by further gelation for 2 hours due to the presence of PLLA and PCL microcrystals. However, knot-like structures as well as continuously smooth pellicles appeared among the nanofibrous network with increasing gelation temperature. DSC analysis indicated that the crystallization of PCL segments was interrupted by rigid PLLA segments, resulting in an amorphous phase at high gelation temperatures. Combining TIPS (thermally induced phase separation) with saltleaching methods, nano-fibrous architecture and interconnected pore structures (144±36 μm in diameter) with a high porosity were created for in vitro culture of chondrocytes. Specific surface area and protein adsorption on the surface of the nano-fibrous scaffold were three times higher than on the surface of the solid-walled scaffold. Chondrocytes cultured on the nano-fibrous scaffold exhibited a spherical condrocyte-like phenotype and secreted more cartilage-like extracellular matrix (ECM) than those cultured on the solid-walled scaffold. Moreover, the protein and DNA contents of cells cultured on the nanofibrous scaffold were 1.2-1.4 times higher than those on the solid-walled scaffold. Higher expression levels of collagen II and aggrecan mRNA were induced on the nanofibrous scaffold compared to on the solid-walled scaffold. These findings demonstrated that scaffolds with a nanofibrous architecture could serve as superior scaffolds for cartilage tissue engineering.

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“…Recently, Jung and colleagues developed an elastic biodegradable poly(L-lactide-co-ε-caprolactone) (PLCL) three-dimensional scaffold and tested it in several cartilage regeneration models (Jung et al, 2008). Although the work mostly considered in vivo investigations, several in vitro tests were also performed.…”

Section: Synthetic-based Polymers As a Scaffold For Culture Of Chondrmentioning

confidence: 99%

“…Moreover, these scaffolds can be used to test the effect of mechanical stimuli on chondrogenic cells. Indeed, several studies showed that different types of mechanical stimulation led to an enhancement of chondrogenic differentiation by cells cultured on these constructs (Jung et al, 2008;Li et al, 2003;Nam et al, 2008;Xie et al, 2007).…”

Section: Synthetic-based Polymers As a Scaffold For Culture Of Chondrmentioning

confidence: 99%

Three-dimensional cultures of osteogenic and chondrogenic cells: A tissue engineering approach to mimic bone and cartilage in vitro

Tortelli

Cancedda²

2009

eCM

103

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Capturing the complexity of bone and cartilage into three-dimensional in vitro models remains one of the most important challenges in the field of the tissue engineering. Indeed, the development and the optimization of novel culture systems may be necessary to face the next questions of bone and cartilage physiology. The models should faithfully mimic these tissues, resembling their organization, their mechanical properties and their physiological response to different stimuli. Here we review the recent advances in the field of the three-dimensional cultures of both osteogenic and chondrogenic cells. In particular, we highlight the most important studies that, to our knowledge, have investigated the response of the cells to the three-dimensional environment provided by the diverse types of scaffold.

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Cartilage regeneration with highly-elastic three-dimensional scaffolds prepared from biodegradable poly(l-lactide-co-ɛ-caprolactone)

Cited by 102 publications

References 25 publications

Evaluation of the Early In Vivo Response of a Functionally Graded Macroporous Scaffold in an Osteochondral Defect in a Rabbit Model

Evaluation of the Early In Vivo Response of a Functionally Graded Macroporous Scaffold in an Osteochondral Defect in a Rabbit Model

Microstructure and properties of nano-fibrous PCL-b-PLLA scaffolds for cartilage tissue engineering

Three-dimensional cultures of osteogenic and chondrogenic cells: A tissue engineering approach to mimic bone and cartilage in vitro

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