Carriage of Staphylococcus species in the veterinary visiting dog population in mainland UK: Molecular characterisation of resistance and virulence

Wedley, Amy; Dawson, Susan; Maddox, Thomas W.; Coyne, Karen P.; Pinchbeck, Gina; Clegg, Peter; Jamrozy, Dorota; Fielder, Mark D.; Donovan, David M.; Nuttall, Tim; Williams, Nicola

doi:10.1016/j.vetmic.2014.01.015

Cited by 40 publications

(48 citation statements)

References 40 publications

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“…Similar to other studies, these findings may reflect the high frequency of contact with these surfaces by a variety of household members, compared to surfaces likely to be unique to the index patient (e.g., bed linens or bath towels) (5,18). In our population, a high proportion of strains recovered from pet dogs and cats were MRSA, consistent with a notable increase in the prevalence of antibioticresistant staphylococcal strains in companion animals over the past decade (19,20).…”

supporting

confidence: 88%

Antimicrobial Susceptibility Profiles of Staphylococcus aureus Isolates Recovered from Humans, Environmental Surfaces, and Companion Animals in Households of Children with Community-Onset Methicillin-Resistant S. aureus Infections

Morelli

Hogan

Sullivan

et al. 2015

Antimicrob Agents Chemother

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Our objective was to determine the antibiotic susceptibility profiles of Staphylococcus aureus isolates recovered from 110 households of children with community-onset methicillin-resistant S. aureus (MRSA) infections. Cultures were obtained from household members, household objects, and dogs and cats, yielding 1,633 S. aureus isolates. The S. aureus isolates were heterogeneous, although more than half were methicillin resistant. The highest proportion of MRSA was found in bathrooms. The majority of isolates were susceptible to antibiotics prescribed in outpatient settings.

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supporting

confidence: 88%

Antimicrobial Susceptibility Profiles of Staphylococcus aureus Isolates Recovered from Humans, Environmental Surfaces, and Companion Animals in Households of Children with Community-Onset Methicillin-Resistant S. aureus Infections

Morelli

Hogan

Sullivan

et al. 2015

Antimicrob Agents Chemother

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“…CoNS were isolated from 74% of clinically healthy dogs. The established prevalence of staphylococci is higher than in other published studies where staphylococci were isolated from 60% (Gandolfi-Decristophoris et al 2013) and 38% of investigated dogs (Wedley et al 2014). While the rate of isolation of CoNS in dogs was highest from the nasal cavity (54.2%), the perianal region also exhibited a relatively high recovery rate for Staphylococcus spp.…”

Section: Discussionmentioning

confidence: 59%

Methicillin-resistant coagulase-negative staphylococci in healthy dogs

Šiugždaitė¹,

Gabinaitienė²

2017

Vet. Med. - Czech

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ABSTRACT:The objective of this study was to evaluate the prevalence of coagulase-negative staphylococci in healthy dogs and to determine whether methicillin-resistant staphylococci expressed the mecA gene. Nasal and rectal swab samples were taken from 50 clinically healthy dogs. The prevalence of coagulase-negative staphylococci was evaluated according to phenotypic properties. The agar diffusion method was applied to evaluate antimicrobial resistance and the prevalence of methicillin resistance was determined using PCR analysing the mecA gene. A total of 59 coagulase-negative staphylococcus strains were isolated from the nostrils and rectums of 37 (74%) clinically healthy dogs. The prevalence of coagulase-negative staphylococci in female dogs was significantly higher compared with male dogs (P < 0.05). The results of antimicrobial susceptibility testing showed that 6.7% of the strains were resistant to oxacillin, 23.7% were resistant to penicillin, 22% to ampicillin and 16.9% to erythromycin. The mecA PCR revealed one oxacillin-sensitive and four oxacillin-resistant coagulase-negative staphylococci strains to be mecA carriers. Staphylococcus sciuri (60%) and Staphylococcus warneri (20%) were the most prevalent species among methicillin-resistant coagulase negative staphylococci. High antimicrobial resistance rates for these bacteria were observed against penicillin (100%), ampicillin (100%), oxacillin (80%), erythromycin (80%) and gentamicin (60%). All strains were susceptible to vancomycin and enrofloxacin. It is assumed that methicillin-resistance genes evolved in coagulase-negative staphylococcus and were then horizontally transferred among staphylococci.

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“…We obtained samples from all humans living and working on the farm, as well as from the nares of companion animals (two dogs and two horses [42,43]). The farmer and farm personnel contributed nasal samples from the vestibule of both anterior nares.…”

Section: Methodsmentioning

confidence: 99%

Eradication of Methicillin-Resistant Staphylococcus aureus and of Enterobacteriaceae Expressing Extended-Spectrum Beta-Lactamases on a Model Pig Farm

Schmithausen

Kellner

Schulze-Geisthoevel

et al. 2015

Appl Environ Microbiol

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g Colonization of livestock with bacteria resistant to antibiotics is considered a risk for the entry of drug-resistant pathogens into the food chain. For this reason, there is a need for novel concepts to address the eradication of drug-resistant commensals on farms. In the present report, we evaluated the decontamination measures taken on a farm contaminated with methicillin-resistant Staphylococcus aureus (MRSA) and Enterobacteriaceae expressing extended-spectrum ␤-lactamases (ESBL-E). The decontamination process preceded the conversion from piglet breeding to gilt production. Microbiological surveillance showed that the decontamination measures eliminated the MRSA and ESBL-E strains that were detected on the farm before the complete removal of pigs, cleaning and disinfection of the stable, and construction of an additional stable meeting high-quality standards. After pig production was restarted, ESBL-E remained undetectable over 12 months, but MRSA was recovered from pigs and the environment within the first 2 days. However, spa (Staphylococcus aureus protein A gene) typing revealed acquisition of an MRSA strain (type t034) that had not been detected before decontamination. Interestingly, we observed that a farmworker who had been colonized with the prior MRSA strain (t2011) acquired the new strain (t034) after 2 months. In summary, this report demonstrates that decontamination protocols similar to those used here can lead to successful elimination of contaminating MRSA and ESBL-E in pigs and the stable environment. Nevertheless, decontamination protocols do not prevent the acquisition of new MRSA strains. N umerous studies have highlighted the emergence of methicillin-resistant Staphylococcus aureus (MRSA) (1-4) and Enterobacteriaceae expressing extended-spectrum ␤-lactamases (ESBL-E) (5-7) in livestock production, particularly in pigs. Hence, there is an ongoing debate whether the use of antibiotics in food animal production represents an important source of continuous spread of MRSA and ESBL-E to humans (8-10). Farmers are confronted with two different consequences of this problem: the potential danger of animal colonization with drug-resistant bacteria for (i) humans living on or in the vicinity of farms (11-13) and for (ii) consumers of animal products (14-18). Indeed, livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) have been found in humans living and working in close contact with pigs and also in hospitals in rural areas (11,(19)(20)(21)(22)(23)(24)(25). In contrast, ESBL-E could possibly be transferred from animals to humans via meat products (16,(26)(27)(28). As a consequence, control points to limit transmission of resistant pathogens "from stable to table" have been demanded (29).Notably, bacteria not only persist on/in the living animal but also on surfaces that are in contact with the animals, such as barn walls and equipment (30, 31). LA-MRSA isolates were detected in dust samples from the investigated breeding farms in Germany as part of the EFSA (European Food Safe...

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Carriage of Staphylococcus species in the veterinary visiting dog population in mainland UK: Molecular characterisation of resistance and virulence

Cited by 40 publications

References 40 publications

Antimicrobial Susceptibility Profiles of Staphylococcus aureus Isolates Recovered from Humans, Environmental Surfaces, and Companion Animals in Households of Children with Community-Onset Methicillin-Resistant S. aureus Infections

Antimicrobial Susceptibility Profiles of Staphylococcus aureus Isolates Recovered from Humans, Environmental Surfaces, and Companion Animals in Households of Children with Community-Onset Methicillin-Resistant S. aureus Infections

Methicillin-resistant coagulase-negative staphylococci in healthy dogs

Eradication of Methicillin-Resistant Staphylococcus aureus and of Enterobacteriaceae Expressing Extended-Spectrum Beta-Lactamases on a Model Pig Farm

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