Carotenoid composition and changes in expression of carotenoid biosynthetic genes in tepals of Asiatic hybrid lily

Yamagishi, Masumi; Kishimoto, Sanae; Nakayama, Masayoshi

doi:10.1111/j.1439-0523.2009.01656.x

Cited by 88 publications

(63 citation statements)

References 33 publications

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“…It should be noted that the accuracy of data may vary among manuscripts. antheraxanthin, violaxanthin, and lutein, while the major carotenoid in the red tepals of the cultivar 'Saija' is capsanthin 47 . The red ketocarotenoid capsanthin is found in the fruits of red pepper 2 but is rarely found in flower petals.…”

Section: Carotenoid Composition In Flower Petalsmentioning

confidence: 99%

Diversity of Carotenoid Composition in Flower Petals

Ohmiya

2011

JARQ

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Carotenoids are yellow, orange, and red pigments that are widely distributed in nature. In plants, carotenoids play important roles in photosynthesis and furnishing flowers and fruits with distinct colors. While most plant leaves show similar carotenoid profiles, containing carotenoids essential for photosynthesis, the carotenoid composition of flower petals varies from species to species. In this review, I present a list of carotenoid composition in the flower petals of various plants and discuss the possible causes of qualitative diversity.

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Section: Carotenoid Composition In Flower Petalsmentioning

confidence: 99%

Diversity of Carotenoid Composition in Flower Petals

Ohmiya

2011

JARQ

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“…To amplify phytoene desaturase (PDS) fragments from N. benthamiana and white lupin, degenerate primers 5′-GCA AGR GAYGTTYTDGGWGG-3′ (forward) and 5′-RTTRCCA TCY AARAADGCC AT-3′ (reverse) were designed, respectively, from the amino acid sequences ARDVLGG and MAFLDGN conserved in plant PDS genes (Yamagishi et al 2010). In all, 505-bp fragments were amplified by reverse transcription (RT)-PCR, cloned into the pGEM T-Easy vector (Promega, Tokyo, Japan), and then sequenced.…”

Section: Isolation Of Pds Fragmentsmentioning

confidence: 99%

<i>Peanut stunt virus</i>-induced gene silencing in white lupin (<i>Lupinus albus</i>)

Yamagishi

Masuta

Suzuki

et al. 2015

Plant Biotechnology

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White lupin (Lupinus albus L.) plants develop cluster roots and have strong resistance to phosphorus starvation. Although many expressed sequences have been identified to explain the mechanisms used by white lupin to acquire phosphorus, the lack of a stable transformation technique has made it challenging to evaluate the functions of these expressed sequences. Virus-induced gene silencing (VIGS) is an attractive method for assaying gene function in species that are difficult to stably transform. Here, we show that the Peanut stunt virus (PSV) vector effectively induces silencing of endogenous genes in white lupin. It is unknown whether PSV is useful for VIGS; therefore, we first inoculated Nicotiana benthamiana plants with PSV harbouring fragments of the N. benthamiana phytoene desaturase gene (NbPDS). Two out of four distinct sequence fragments of NbPDS induced photo-bleaching in N. benthamiana, indicating that PSV can be used to knockdown endogenous gene sequences in a sequence-dependent manner. White lupin plants inoculated with PSV harbouring fragments of the L. albus PDS gene (LaPDS) developed photo-bleaching that was associated with a significant reduction in LaPDS mRNA accumulation. PSV spread systemically in leaves, roots, and cluster roots, and small interfering RNA of LaPDS was detected in these organs. This is the first study to demonstrate the use of VIGS by PSV, suggesting that this vector can be applied to supress endogenous gene expression in shoots and roots of white lupin and to clarify the mechanisms of phosphorus starvation resistance.

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“…Moreover, carotenoids are associated with flower colors, which may change with the relative proportions of individual carotenoids. For example, the main carotenoid in red lily flower petals is capsanthin, whereas a combination of violaxanthin, lutein, antheraxanthin, and β-carotene was observed in pink lily flowers (Yamagishi et al, 2010). The ratio of transcript abundance of specific carotenoid biosynthetic genes is probably important for the determination of flower color.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning of BnZEP and its expression in petals of different colors inBrassica napus L.

Jiang¹,

Tian²,

Guo³

et al. 2015

Turk J Agric For

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Carotenoids are a class of pigment elements present in photosynthetic organisms. The transcripts of specific carotenoid biosynthetic genes are probably important for the formation of different colors of flower petals. Zeaxanthin epoxidase (ZEP) enzyme activity is important for the high content of carotenoids in petals. In the present study, the zeaxanthin epoxidase gene in Brassica napus L. (BnZEP) was cloned and bioinformatically analyzed. The expression levels of the BnZEP gene in different plant organs and petals of different colors were investigated and compared in B. napus. The results showed that the full-length cDNA sequence of BnZEP was 2228 bp, containing an open reading frame of 2010 bp and encoding a putative protein of 669 amino acids, with a molecular weight of 73.8 kDa and an isoelectric point (pI) of 6.16. The full-length genomic DNA sequence of BnZEP was 3268 bp, containing 16 exons and 15 introns, the same as in Arabidopsis thaliana (AtZEP). The predicted amino acid sequence of BnZEP revealed a close phylogenetic relationship among the cruciferous species, including Arabidopsis thaliana. The expression levels of BnZEP were remarkably different in different organs and in petals of different colors. Obviously higher expression levels of BnZEP were found in flower petals than in leaves and buds. Yellow petals had markedly higher expression levels of BnZEP than white petals. The results showed that the expression level of BnZEP was positively correlated with yellow petal color in B. napus L. However, the highest expression level of BnZEP was observed in the pale yellow flower petals of the F 1 hybrid (HW243 × ZHONGYOU 821). This implied that the expression level of BnZEP was not completely related to the degree of yellow petal color in B. napus L. Based on the roles of ZEP enzyme in the xanthophyll cycle, it was suggested that the yellow flower color in Brassica napus L. was probably associated with the accumulation of antheraxanthin and violaxanthin in the petals. However, further studies are necessary to identify the specific key carotenoid(s) for the yellow color in the petals of Brassica napus L.

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Carotenoid composition and changes in expression of carotenoid biosynthetic genes in tepals of Asiatic hybrid lily

Cited by 88 publications

References 33 publications

Diversity of Carotenoid Composition in Flower Petals

Diversity of Carotenoid Composition in Flower Petals

<i>Peanut stunt virus</i>-induced gene silencing in white lupin (<i>Lupinus albus</i>)

Molecular cloning of BnZEP and its expression in petals of different colors inBrassica napus L.

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