Diversity of Carotenoid Composition in Flower Petals

Ohmiya, Akemi

doi:10.6090/jarq.45.163

Cited by 71 publications

(78 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Eugster and Märki-Fischer (1991) could identify nearly 40 different carotenoids in the extract of rose petals, most prominently Violaxanthin together with Auroxanthin, Luteoxanthin and ß-Carotene (Ohmiya, 2011). Glick (2009) identified as main components Violaxanthin and Neoxanthin, which comprised 85% of the total carotenoid content in the petals of the rose cultivar “Frisco.” The biosynthetic pathway to Violaxanthin occurs via Zeaxanthin, and the modification is catalyzed by the enzyme Zeaxanthin epoxidase (ZEP).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Analysis of the Anthocyanin and Carotenoid Contents of Rose Petals

et al. 2016

View full text Add to dashboard Cite

Petal color is one of the key characteristics determining the attractiveness and therefore the commercial value of an ornamental crop. Here, we present the first genome-wide association study for the important ornamental crop rose, focusing on the anthocyanin and carotenoid contents in petals of 96 diverse tetraploid garden rose genotypes. Cultivated roses display a vast phenotypic and genetic diversity and are therefore ideal targets for association genetics. For marker analysis, we used a recently designed Axiom SNP chip comprising 68,000 SNPs with additionally 281 SSRs, 400 AFLPs and 246 markers from candidate genes. An analysis of the structure of the rose population revealed three subpopulations with most of the genetic variation between individual genotypes rather than between clusters and with a high average proportion of heterozygous loci. The mapping of markers significantly associated with anthocyanin and carotenoid content to the related Fragaria and Prunus genomes revealed clusters of associated markers indicating five genomic regions associated with the total anthocyanin content and two large clusters associated with the carotenoid content. Among the marker clusters associated with the phenotypes, we found several candidate genes with known functions in either the anthocyanin or the carotenoid biosynthesis pathways. Among others, we identified a glutathione-S-transferase, 4CL, an auxin response factor and F3'H as candidate genes affecting anthocyanin concentration, and CCD4 and Zeaxanthine epoxidase as candidates affecting the concentration of carotenoids. These markers are starting points for future validation experiments in independent populations as well as for functional genomic studies to identify the causal factors for the observed color phenotypes. Furthermore, validated markers may be interesting tools for marker-assisted selection in commercial breeding programmes in that they provide the tools to identify superior parental combinations that combine several associated markers in higher dosages.

show abstract

Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Analysis of the Anthocyanin and Carotenoid Contents of Rose Petals

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Several investigations of the carotenoid composition of petals have been reported [24]). Although the leaves of most plants have similar carotenoid compositions, the carotenoid composition in petals are species-specific, indicating different regulatory mechanisms affecting the carotenoid biosynthesis pathway in different plant organs [25]. Many flowers with white petals contain few carotenoid molecules, whereas the dark orange petals could accumulate up to several-fold the carotenoid content of leaves [26].…”

Section: Introductionmentioning

confidence: 99%

Crocins with High Levels of Sugar Conjugation Contribute to the Yellow Colours of Early-Spring Flowering Crocus Tepals

et al. 2013

View full text Add to dashboard Cite

Crocus sativus is the source of saffron spice, the processed stigma which accumulates glucosylated apocarotenoids known as crocins. Crocins are found in the stigmas of other Crocuses, determining the colourations observed from pale yellow to dark red. By contrast, tepals in Crocus species display a wider diversity of colours which range from purple, blue, yellow to white. In this study, we investigated whether the contribution of crocins to colour extends from stigmas to the tepals of yellow Crocus species. Tepals from seven species were analysed by UPLC-PDA and ESI-Q-TOF-MS/MS revealing for the first time the presence of highly glucosylated crocins in this tissue. β-carotene was found to be the precursor of these crocins and some of them were found to contain rhamnose, never before reported. When crocin profiles from tepals were compared with those from stigmas, clear differences were found, including the presence of new apocarotenoids in stigmas. Furthermore, each species showed a characteristic profile which was not correlated with the phylogenetic relationship among species. While gene expression analysis in tepals of genes involved in carotenoid metabolism showed that phytoene synthase was a key enzyme in apocarotenoid biosynthesis in tepals. Expression of a crocetin glucosyltransferase, previously identified in saffron, was detected in all the samples. The presence of crocins in tepals is compatible with the role of chromophores to attract pollinators. The identification of tepals as new sources of crocins is of special interest given their wide range of applications in medicine, cosmetics and colouring industries.

show abstract

“…In addition, these carotenoids exist in a free form in the chloroplast. In contrast, the carotenoid composition in flowers varies with plant species (Ohmiya 2011). Carotenoids that accumulate in the chromoplast are in a more oxidized form than those in the chloroplast, and they typically exist in an esterified form.…”

Section: Regulation Of Carotenoid Accumulation In Petals Of Species Omentioning

confidence: 95%

Anthocyanin and carotenoid pigmentation in flowers of section Mina, subgenus Quamoclit, genus Ipomoea

2012

Self Cite

View full text Add to dashboard Cite

Plants belong to the section Mina showed characteristic petal pigmentation among Ipomoea plants. For example, petals of Ipomoea hederifolia var. lutea display yellow color derived from carotenoids and those of Ipomoea quamoclit display red color derived from pelargonidin-based anthocyanins. In this study, the pigment composition and the expression patterns of genes for pigment biosynthesis in the petals of I. quamoclit and I. hederifolia var. lutea were analyzed to elucidate the crucial factors that determine petal color in section Mina. The petals of whiteflowered I. quamoclit lack the ability to synthesize anthocyanins and carotenoids because of the suppression of anthocyanidin synthase (ANS) gene expression and transcriptional down-regulation of multiple carotenogenic genes, respectively. In petals of I. hederifolia var. lutea, the absence of dihydroflavonol 4-reductase (DFR) gene expression is responsible for the lack of anthocyanins. All F 1 progeny obtained by interspecies crossing between I. quamoclit and I. hederifolia var. lutea had scarlet petal color produced by a combination of pelargonidin-based anthocyanins and carotenoids. The flavanone 3-hydroxylase gene, DFR, and ANS were expressed in the petals of the F 1 progeny. The results suggest that in these section Mina species, the carotenoid-accumulating phenotype in petals is dominant, and that pelargonidin was produced in the F 1 progeny by complementary expression of DFR from white-flowered I. quamoclit and ANS from I. hederifolia var. lutea.

show abstract

Diversity of Carotenoid Composition in Flower Petals

Cited by 71 publications

References 45 publications

Genome-Wide Association Analysis of the Anthocyanin and Carotenoid Contents of Rose Petals

Genome-Wide Association Analysis of the Anthocyanin and Carotenoid Contents of Rose Petals

Crocins with High Levels of Sugar Conjugation Contribute to the Yellow Colours of Early-Spring Flowering Crocus Tepals

Anthocyanin and carotenoid pigmentation in flowers of section Mina, subgenus Quamoclit, genus Ipomoea

Contact Info

Product

Resources

About