Carotenogenic gene expression and ultrastructural changes during development in marigold

Villar‐Martínez, Alma Angélica Del; García-Saucedo, Pedro Antonio; Cárabez-Trejo, Alfonso; Cruz-Hernández, Andrés; Paredes‐López, Octavio

doi:10.1016/j.jplph.2004.12.004

Cited by 31 publications

(16 citation statements)

References 39 publications

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“… 52 Although β-carotene production during melon fruit ripening is associated with the differential regulation of carotenogenic genes, similar levels and patterns of gene expression are observed between white and orange fruit (Chayut et al, 2015, manuscript submitted). Carotenoid accumulation disjoint from differential gene transcription has also been reported in many other cases, such as between white- and orange-fleshed squash, 51 during the fruit ripening of normal and red orange, 57 in yellow- versus red-fleshed watermelon, 21 and in flowers, such as between white and yellow marigold, 99 between white chrysanthemum and its yellow bud mutant, 65 and in lilies, 68 indicating the existence of different, additional regulatory mechanisms.…”

Section: Transcriptional Regulation Of the Carotenoid Biosynthetic Gementioning

confidence: 83%

Carotenoid metabolism and regulation in horticultural crops

et al. 2015

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Carotenoids are a diverse group of pigments widely distributed in nature. The vivid yellow, orange, and red colors of many horticultural crops are attributed to the overaccumulation of carotenoids, which contribute to a critical agronomic trait for flowers and an important quality trait for fruits and vegetables. Not only do carotenoids give horticultural crops their visual appeal, they also enhance nutritional value and health benefits for humans. As a result, carotenoid research in horticultural crops has grown exponentially over the last decade. These investigations have advanced our fundamental understanding of carotenoid metabolism and regulation in plants. In this review, we provide an overview of carotenoid biosynthesis, degradation, and accumulation in horticultural crops and highlight recent achievements in our understanding of carotenoid metabolic regulation in vegetables, fruits, and flowers.

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Section: Transcriptional Regulation Of the Carotenoid Biosynthetic Gementioning

confidence: 83%

Carotenoid metabolism and regulation in horticultural crops

et al. 2015

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“…Samples for TEM analyses were taken from green and yellow pigmented sections of ten calli. Samples 200-500 nm thick were obtained from the center of each callus, keeping the same orientation of horizontal, parallel to the observation plane (Del Villar-Martínez et al 2005).…”

Section: Methodsmentioning

confidence: 99%

“…There have been no reports of this kind in T. erecta cells cultured in vitro. The most related report was published by Del Villar-Martínez et al (2005), where chromoplasts were analyzed from ligules of plants with different pigmentation levels. Therefore, the main contribution from our work is the morphometrical descriptions of plastids in cell culture and the corresponding cell shape relationship, with pigment accumulation.…”

Section: )mentioning

confidence: 99%

“…In addition, marigold is an important source of carotenoids for the food industry (Del Villar-Martínez et al 2009). In marigold, lutein is the main pigment that accumulates in flowers, with color differences between varieties resulting from differences in lutein content (Delgado-Vargas and Paredes-López 2003;Del Villar-Martínez et al 2005). From subcellular analyses, changes occurring during plastid differentiation and during marigold floral morphogenesis have demonstrated that pigment deposition occurs in specific structures, termed lipidic vesicles (Del Villar-Martínez et al 2005).…”

Section: Introductionmentioning

confidence: 99%

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Plastid analysis of pigmented undifferentiated cells of marigold Tagetes erecta L. by transmission electron microscopy

Vanegas‐Espinoza¹,

Ramos-Viveros²,

Jiménez–Aparicio³

et al. 2011

In Vitro Cell.Dev.Biol.-Plant

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Marigold (Tagetes erecta) flowers are primarily used in industry for their high pigment content. Flower color development implies that chloroplast-chromoplast transition is associated with carotenoid biosynthesis. We report the recovery of undifferentiated pigmented marigold cells, various callus tissues, and their analysis by transmission electron microscopy in order to observe accumulating pigment and development of subcellular structures. Callus was generated from leaf explants and after several rounds of recurrent selection. Green-, yellow-, and brown-colored callus were obtained that showed distinct carotenoid profiles. For green material, violaxanthin, lutein, zeaxanthin, and β-carotene were produced, while yellow callus generated mainly lutein, as did the brown callus. Chloroplastchromoplast transition was followed by measuring plastid size and shape in undifferentiated marigold cells by digital image analysis. Cellular alterations were evident in brown callus. Chloroplasts were the main structure in green callus, while yellow callus clearly showed the formation of plastoglobules, structures that are correlated with chloroplast-chromoplast transition. The high number of plastoglobules observed in yellow callus is possibly directly related to pigment synthesis and accumulation.

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“…T. erecta has been used for centuries as an ornamental and medicinal plant, and researchers currently seek to increase the pigment content. There are reports about quantification of psy, pds, lcy-β and lcy-ε gene expression about T. erecta (Del Villar Martínez et al 2005;Moehs et al 2001); studies on in vitro tissue and cell cultures are mostly interested in analyzing the influence of medium factors on callus induction or/and regeneration. This kind of research is necessary in order to work on genetic manipulation, defining the appropiated stage and cell type for modified plants regeneration (Bespalhok and Hattori 1999;Von Arnold 2008).…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis from leaf explants of <i>Tagetes erecta</i> L.

Espinoza

Benítez-García

Peralta

et al. 2017

Plant Biotechnology

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Tagetes erecta is an asteraceous plant of industrial, ornamental and medicinal importance; its inflorescences have been used as a pigment source for food coloring, mainly for poultry skin and eggs. Nevertheless, there are few reports on plant regeneration or micropropagation, because unsuccesfull results in the plant's reaction to the growth regulators, developing embryogenesis on Tagetes erecta. In this study, somatic embryogenesis was induced and plantlets of Tagetes erecta were regenerated. For induction of globular structures MS medium supplemented with 2,4-D (4.5 µM) and BAP (8.8 µM) was used; globular structures were transferred to MS medium with 45 g l −1 sucrose until the embryos maturation. Transmission electron microscopy showed characteristic subcellular structures of embryogenic callus. Somatic embryos were transferred to MS medium without plant growth regulators and whole plantlets were obtained. In vitro plants were successfully transplanted into a mixture of peat moss and vermiculite (1 : 1 v/v) under greenhouse conditions. In this study, somatic embryogenesis and plant regeneration system from foliar explants were established, an important requirement for performing genetic transformation events on Tagetes erecta.

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Carotenogenic gene expression and ultrastructural changes during development in marigold

Cited by 31 publications

References 39 publications

Carotenoid metabolism and regulation in horticultural crops

Carotenoid metabolism and regulation in horticultural crops

Plastid analysis of pigmented undifferentiated cells of marigold Tagetes erecta L. by transmission electron microscopy

Somatic embryogenesis from leaf explants of <i>Tagetes erecta</i> L.

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