Carnitina libre y total en sangre de cordón umbilical

Osório, José Henry; Pourfarzam, Morteza

doi:10.18597/rcog.633

Cited by 1 publication

(1 citation statement)

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“…On the other hand, Talián et al ( 18 ) employed dried cord plasma spotted on filter paper cards. Osorio and Pourfarzam ( 20 ) favored this specimen for the analysis of total and free carnitine but turned to DBS of whole CB in order to obtain the full acylcarnitine profile ( 19 ). Chace et al ( 21 ) compared the results for total and free carnitine in whole cord blood and plasma, both spotted on filter paper.…”

Section: Discussionmentioning

confidence: 99%

Analysis of acylcarnitine profiles in umbilical cord blood and during the early neonatal period by electrospray ionization tandem mass spectrometry

Neto

Fonseca²,

Almeida³

et al. 2012

Braz J Med Biol Res

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Acylcarnitine profiling by electrospray ionization tandem mass spectrometry (ESI-MS/MS) is a potent tool for the diagnosis and screening of fatty acid oxidation and organic acid disorders. Few studies have analyzed free carnitine and acylcarnitines in dried blood spots (DBS) of umbilical cord blood (CB) and the postnatal changes in the concentrations of these analytes. We have investigated these metabolites in healthy exclusively breastfed neonates and examined possible effects of birth weight and gestational age. DBS of CB were collected from 162 adequate for gestational age neonates. Paired DBS of heel-prick blood were collected 4-8 days after birth from 106 of these neonates, the majority exclusively breastfed. Methanol extracts of DBS with deuterium-labeled internal standards were derivatized before analysis by ESI-MS/MS. Most of the analytes were measured using a full-scan method. The levels of the major long-chain acylcarnitines, palmitoylcarnitine, stearoylcarnitine, and oleoylcarnitine, increased by 27, 12, and 109%, respectively, in the first week of life. Free carnitine and acetylcarnitine had a modest increase: 8 and 11%, respectively. Propionylcarnitine presented a different behavior, decreasing 9% during the period. The correlations between birth weight or gestational age and the concentrations of the analytes in DBS were weak ( r ≤ 0.20) or nonsignificant. Adaptation to breast milk as the sole source of nutrients can explain the increase of these metabolites along the early neonatal period. Acylcarnitine profiling in CB should have a role in the early detection of metabolic disorders in high-risk neonates.

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Section: Discussionmentioning

confidence: 99%