Caries prevalence associated with Streptococcus mutans and Streptococcus sobrinus in Japanese schoolchildren

Okada, Morihiko; Kawamura, Makoto; Oda, Yoichi; Yasuda, Rie; Kojima, Takanori; Kurihara, Hidemi

doi:10.1111/j.1365-263x.2011.01203.x

Cited by 39 publications

(38 citation statements)

References 43 publications

(62 reference statements)

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“…The present findings show that the prevalence of mutans streptococci was 94.5% in subjects aged 11-36 years, which is consistent with the findings of a previous study of Down syndrome patients aged 1-48 years (29). However, other studies have reported lower prevalence rates for mutans streptococci in children (13,20,27,39). Because S. sobrinus becomes established later than S. mutans in the oral cavity of children older than 3 years (40), it is likely that the retention rate for S. sobrinus in the permanent dentition of children with ID would be greater than in normal children.…”

Section: Discussionsupporting

confidence: 83%

“…In addition, a recent report noted that, in schoolchildren and adults with ID, dental caries experience was significantly greater in both permanent and primary teeth among those harboring both S. mutans and S. sobrinus than among those with S. mutans alone (13,23), which confirmed the findings of a similar study that used real-time quantitative reverse transcriptase-PCR (qRT-PCR) methods to investigate cultures (24,25).…”

Section: Introductionsupporting

confidence: 78%

“…Dental plaque was collected from all erupted teeth by brushing with a sterile toothbrush for 1 min, as previously described (13). The toothbrush was washed several times in a tube with 15 mL of sterile distilled water, to remove adherent plaque.…”

Section: Plaque Samplingmentioning

confidence: 99%

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Five-year longitudinal study of dental caries risk associated with Streptococcus mutans and Streptococcus sobrinus in individuals with intellectual disabilities

et al. 2017

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Streptococcus mutans (S. mutans) andStreptococcus sobrinus (S. sobrinus) are important etiologic agents in human dental caries. Using quantitative real-time polymerase chain reaction assays for the presence of those strains, we examined 145 outpatients with intellectual disability (ID), calculated the proportion of each of these strains to total bacteria, and compared dental caries incidence over 5 years. Plaque samples were collected from all erupted tooth sites, and dental examinations were performed annually to determine numbers of decayed, missing, and filled teeth (DMFT score; World Health Organization caries diagnostic criteria). Elevated DMFT scores were calculated as ∆DMFT, and sites of newly affected caries (∆SNAC) were identified. Sixty-six patients had both strains. The proportion of S. mutans to total bacteria was moderately correlated with DMFT in year 2, ∆DMFT in years 2 and 5, and ∆SNAC in years 2 and 5 (correlation coefficient = 0.470, P < 0.001), while the proportion of S. sobrinus to total bacteria was moderately correlated with DMFT in years 2 and 5, ∆DMFT in years 1, 2, and 5, and ∆SNAC in years 2 and 5 (correlation coefficient = 0.695, P < 0.001). Individuals with ID who harbored both bacterial strains had a higher risk of dental caries and a significantly higher proportion of S. sobrinus to total bacteria.

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Section: Discussionsupporting

confidence: 83%

Section: Introductionsupporting

confidence: 78%

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Five-year longitudinal study of dental caries risk associated with Streptococcus mutans and Streptococcus sobrinus in individuals with intellectual disabilities

et al. 2017

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“…In this study, supra gingival plaque samples were collected from the cervical margins of both caries and caries free tooth surfaces with the help of a sterile dental explorer. Various authors as Carmona et al [9], Choi et al [10] and Okada et al [11], in their respective studies collected supra gingival plaque samples with the dental explorer for the detection of S. mutans and S. sobrinus from children without caries, with early childhood caries and severe early childhood caries.…”

Section: Discussionmentioning

confidence: 99%

Distribution of S. mutans and S. sorbinus in Caries Active and Caries Free Children by PCR Approach

Soni¹,

Vasavada²

2015

Int J Oral Craniofac Sci

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Background: Streptococcus mutans (S. mutans) and Streptococcus sorbinus (S. sorbinus) have been considered to be the most important micro-organisms associated with dental caries. Therefore, purpose of this study is to detect and correlate the presence of S. mutans and S. sobrinus in the dental plaque of caries free and caries active children, by using Polymerase chain reaction (PCR) method. Materials and Methods:Twenty patients aged between ages 4-8 years were included in the study. The subjects were divided in two groups: Group A consisting of ten children with early childhood caries and Group B consisting of ten caries-free children. Dental examinations were performed using a plane dental mirror and explorer. Plaque sample was collected from the cervical margin of the teeth by using explorer. PCR testing was performed for S. mutans and S. sorbinus. The data was analyzed by using SPSS software. The caries score data was analyzed among groups by applying Fischer's exact test. The P value <0.05 was considered as significant.Results: S. mutans was present in all patients, whether they were caries active or caries free. There was no statistically significant difference in the presence of S. sorbinus in caries active patients as compared to caries free patients. It was also noticed that S. sorbinus was found more in male patients as compared to females, the difference being statistically non-significant. Conclusion:Children harbouring both S. mutans and S. sorbinus had a higher incidence of dental caries as compared to the presence of either organism alone.Among the oral bacteria, Mutans streptococci have been implicated as a major cariogenic bacteria. The degree of colonization of these organisms correlates with the prevalence of dental caries in children and experimental animals.Mutans streptococci is divided into seven species: Streptococcus mutans, Streptococcus sobrinus, Streptococcus downei, Streptococcus rattus, Streptococcus cricetus, Streptococcus ferus, and Streptococcus macacae. Among these S. mutans and S. sobrinus are strongly associated with human dental caries [6]. Epidemiological studies have reported that S. mutans is more prevalent than S. sorbinus in the oral cavity, but have also shown that the prevalence of S. sorbinus is more closely associated with a high caries experience [7].Molecular methods for bacterial identification and enumeration now make it more precise to study the microbiota associated with caries. DNA sequence-based assays can be used to identify closely related species that are difficult to differentiate by traditional, culturebased approaches [8]. Various methods have been used for detection, including biochemical tests, immunological tests, DNA probes, and Polymerase Chain Reaction (PCR). Among these, the PCR method is currently being applied to the detection of putative pathogens and the identification of human cariogenic bacteria because it is rapid, sensitive, and simple.The purpose of this study is to detect and correlate the presence of S. mutans and S. sobrinus in...

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“…Epidemiological studies have shown a correlation between the presence of Streptococcus mutans and Streptococcus sobrinus with caries incidence [1][2][3][4] . In early childhood caries, the presence of both microorganisms promoted a significantly higher caries increment than S. mutans alone 5 .…”

Section: Introductionmentioning

confidence: 99%

Effect of dietary sugars on dual-species biofilms of Streptococcus mutans and Streptococcus sobrinus – a pilot study

Oliveira

Albuquerque

Spolidório

et al. 2016

Rev. odontol. UNESP

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ResumoIntrodução: O consumo frequente de açucares e a presença de Streptococcus mutans e Streptococcus sobrinus estão correlacionados com maior experiência de cárie. Objetivo: Elucidar o efeito de diferentes carboidratos fermentáveis na biomassa e acidogenicidade de biofilmes formados por S. mutans e S. sobrinus. Material e método: Biofilmes única e dupla-espécie de S. mutans ATCC 25175 e S. sobrinus ATCC 27607 em concentrações iguais cresceram no fundo de placas de microtitulação por 24 h a 37 °C em microaerofilia. Maltose, sacarose, glicose e lactose foram adicionados a 2%. BHI caldo (0.2% glicose) foi usado como controle negativo. Acidogenicidade foi avaliada por meio da medição do pH do meio de cultura após 24 h, imediatamente após troca de meio e nas próximas 1 h e 2 h. Coloração por cristal violeta foi usada como indicador do total de biomassa aderida, após 24 h de incubação. Os dados foram analisados por teste ANOVA two way e Teste de Bonferroni. O nível de significância foi de 5%. Resultado: Todos os carboidratos resultaram em maior formação de biomassa em ambos os tipos de biofilme (única ou dupla-espécie), quando comparado ao grupo controle. Sacarose, lactose e maltose mostraram maior acidogenicidade que o grupo controle após 24 h nos biofilmes única ou dupla-espécie, apenas após 24 h. Conclusão: Os achados indicam que o tipo de biofilme (única ou dupla-espécie) e o tipo de carboidrato usado podem influenciar tanto na quantidade de biomassa formada quanto na taxa de redução do pH.Descritores: Biofilme; biomassa; Streptococcus mutans; Streptococcus sobrinus. AbstractIntroduction: Frequent consumption of sugars and the presence of Streptococcus mutans and Streptococcus sobrinus are correlated with higher caries experience. Objective: The aim of this pilot study was to elucidate the effect of different fermentable carbohydrates on biomass formation and acidogenicity of S. mutans and S. sobrinus biofilms. Material and method: Single and dual-species biofilms of S. mutans ATCC 25175 and S. sobrinus ATCC 27607 were grown at the bottom of microtiter plates at equal concentrations for 24 h at 37 °C under micro-aerobic atmosphere. Carbohydrates were added at 2% concentration: maltose, sucrose, glucose and lactose. BHI Broth (0.2% glucose) was used as negative control. Acidogenicity was assessed by measuring the pH of spent culture medium after 24 h, immediately after refreshing the culture medium and for the next 1 h and 2 h. Crystal violet staining was used as an indicator of the total attached biofilm biomass after 24 h incubation. Data were analyzed by two-way ANOVA followed by Bonferroni post hoc test. Significance level was set at 5%. Result: All carbohydrates resulted in higher biomass formation in single-and dual-species biofilms when compared to the control group. Sucrose, lactose and maltose showed higher acidogenicity than the control group in both single-and dual-species biofilms after 24 h. Conclusion: These findings indicate that the type of biofilm (single-or dual-species) and the carbohydrate use...

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Caries prevalence associated with Streptococcus mutans and Streptococcus sobrinus in Japanese schoolchildren

Abstract: These results suggest that schoolchildren harbouring both S. mutans and S. sobrinus have a significant higher dental caries experience in both permanent and primary teeth as compared to those with S. mutans alone.

Cited by 39 publications

References 43 publications

Five-year longitudinal study of dental caries risk associated with <i>Streptococcus mutans</i> and <i>Streptococcus sobrinus</i> in individuals with intellectual disabilities

Five-year longitudinal study of dental caries risk associated with <i>Streptococcus mutans</i> and <i>Streptococcus sobrinus</i> in individuals with intellectual disabilities

Distribution of S. mutans and S. sorbinus in Caries Active and Caries Free Children by PCR Approach

Effect of dietary sugars on dual-species biofilms of Streptococcus mutans and Streptococcus sobrinus – a pilot study

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