Cardiovascular pharmacology of 3-n-butylphthalide in spontaneously hypertensive rats

Abstract

The hypotensive and vasorelaxant effects of 3‐n ‐butylphthalide (BuPh) and its possible mechanisms of action were investigated in spontaneously hypertensive rats (SHR) for the first time. A 13‐day intraperitoneal infusion of BuPh at doses of 2.0 and 4.0 mg/day produced a transient hypotensive effect while a dose of 0.5 mg/day showed a significant hypotensive effect only on day 12. BuPh at 0.5 mg/day had no effect on the plasma and tissue angiotensin converting enzyme (ACE) activities, or on the tissue lipid peroxidation index. BuPh relaxed endothelium‐intact and denuded aortic rings precontracted with phenylephrine and KCl. NG‐nitro‐L‐arginine methyl ester, an inhibitor of nitric oxide synthase, did not attenuate the vasorelaxant activity of BuPh. The cumulative concentration response curves of phenylephrine and Ca2+ (in CaCl2‐free, high KCl medium) were non‐competitively inhibited by BuPh. However, BuPh did not interfere with the caffeine‐induced release of intracellular Ca2+. It appears that the vasorelaxant effect of BuPh could be attributed to the blockade of Ca2+ entry, possibly through voltage‐ and receptor‐operated Ca2+ channels, thereby lowering the systolic blood pressure of SHR. © 1997 John Wiley & Sons, Ltd.

Safety Evaluation and Biochemical Efficacy of Celery Seed Extract (Apium Graveolens) Capsules in Hypertensive Patients: A Randomized, Triple-blind, Placebo-Controlled, Cross-Over, Clinical Trial

“…Moreover, a significant reduction in BP, due to NBP administration, was observed in the chronic kidney disease model against hypertensive nephropathy using spontaneously hypertensive rats [30]. In another study, the hypotensive effects of NBP were reported in vivo model which significantly decreased BP [33]. In the present clinical study, celery capsules had no significant effect on blood cells including WBC, RBC, platelet, and their indices in comparison with placebo treatment (P>0.05).…”

Plants Used as Antihypertensive

“…This is because of betterpreservation of n-butylphthalide, which is responsible for the flavor and aromatic odor of celery. This effect of n-butylphthalide was also supported by SHRs [54]. According to Ko et al apigeninflavoneextracted form effect voltage and receptor gated channels via blocking of Ca 2+ influxwhich in result block aortic ring contractions in the isolated aorta of rat [55].…”

Anti-hypertensive Herbs and their Mechanisms of Action: Part I

“…This was explained by greater retention of n-butylphthalide, which has been identified as the source of celery's flavor and aromatic odor (Moghadam et al, 2013 ). Similarly, this phthalide was reported to decrease BP in another animal model of hypertension (SHRs; Tsi and Tan, 1997 ).…”