Cardioprotective effects of <i>Galium verum</i> L. extract against myocardial ischemia-reperfusion injury

Bradić, Jovana; Jeremić, Nevena; Petkovic, Anica; Jeremić, Jovana; Živković, Vladimir; Srejović, Ivan; Sretenovic, Jasmina; Matić, Sanja; Jakovljević, Vladimir; Tomović, Marina

doi:10.1080/13813455.2018.1551904

Cited by 11 publications

(15 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The stimulation of hypertension and coronary heart disease leads to hypertrophy, fibrosis and apoptosis of cardiomyocytes. At the same time, the expressions of marker genes also change, resulting in decreased contractility and enlargement of the myocardium, which eventually lead to heart failure [7]. The prevention and treatment of ischemia reperfusion injury (one of the postoperative complications of coronary artery treatment in clinical practice) has received extensive attention, with continuous development of various clinically-difficult cardiac techniques [8].…”

Section: Discussionmentioning

confidence: 99%

Morphine pretreatment reduces myocardial ischemiareperfusion injury in heart failure rats via GSK-3β/Cx43 signaling proteins and apoptosis-related gene, Bcl-2/Bax

Zhu¹,

Geng²,

Han³

et al. 2020

Trop. J. Pharm Res

View full text Add to dashboard Cite

Purpose: To investigate the effect of morphine preconditioning on myocardial ischemia reperfusion injury in heart failure rats, and the mechanism(s) of action involvedMethods: Seventy-two healthy male Sprague-Dawley rats were assigned to 4 groups: sham, model, morphine-preconditioning and SB203580 inhibitor groups, each with 18 rats. The expressions of P-p38, p-glycogen synthetase kinase-3, and p-gap junction protein 43 in rat myocardial cells were assayed by Western blotting. The mRNA expression levels of Bcl-2 and Bax, and Bcl-2/Bax were determined using real-time fluorescence quantitative PCR.Results: The expression levels of P-p38, p-glycogen synthetase kinase-3, p-gap junction protein 43, Bcl-2 mRNA and Bcl-2/Bax were significantly higher in the pretreatment group than in the model group, while Bax mRNA was significantly lower (p < 0.05). Moreover, the mRNA expression levels of P-p38, pglycogen synthetase kinase-3, p-gap junction protein, Bcl-2, and Bcl-2/Bax in inhibitor-treated rats decreased significantly, when compared to the values for pretreatment rats; furthermore, Bax mRNA was markedly upregulated (p < 0.05).Conclusion: Morphine preconditioning significantly inhibits the expressions of GSK-3β and Cx43 signaling proteins, as well as apoptosis-related gene, Bcl-2 and Bax. In addition, it inhibits the apoptosis of rat cardiomyocytes, and reduces myocardial injury, after ischemia reperfusion, via activation of the p38 MARK signaling pathway. This provides a new strategy for clinical reduction of myocardial injury after ischemia-reperfusion. Keywords: Morphine, Pretreatment, GSK-3β/Cx43 signaling protein, Bcl-2/Bax, Heart failure, Ischemiareperfusion injury

show abstract

Section: Discussionmentioning

confidence: 99%

Morphine pretreatment reduces myocardial ischemiareperfusion injury in heart failure rats via GSK-3β/Cx43 signaling proteins and apoptosis-related gene, Bcl-2/Bax

Zhu¹,

Geng²,

Han³

et al. 2020

Trop. J. Pharm Res

View full text Add to dashboard Cite

show abstract

“…A section of each cardiac tissue (0.5 g) was homogenized in 5 mL phosphate buffer (pH 7.4) by an electrical homogenizer, on ice. Homogenates were centrifuged (1200× g , 4°C, 20 min), obtained supernatants were isolated and stored at −80°C until the determination of biochemical markers (TBARS and antioxidative defence parameters such as superoxide dismutase (SOD), reduced glutathione (GSH) and catalase (CAT); Bradic et al, 2020). The degree of the TBARS in the heart tissue was measured as described previously (Ohkawa et al, 1979).…”

Section: Methodsmentioning

confidence: 99%

Cardiac, biochemical and histopathological analysis reveals impaired heart function in hypertensive rats with apical periodontitis

et al. 2021

Self Cite

View full text Add to dashboard Cite

Aim To investigate the association between experimentally induced apical periodontitis (AP) and heart function in hypertensive rats. Methodology Forty‐eight normotensive Wistar albino and spontaneously hypertensive (SHR) rats were divided into four equal groups: control (C), normotensive with AP (AP), SHR and SHR with AP (SHR + AP). AP was induced on the first right mandibular molars by exposing the pulp chambers to the oral environment for four weeks and confirmed radiographically. Thereafter, the animals were sacrificed by cervical dislocation, whilst hearts were isolated and perfused according to the Langendorff technique gradually increasing coronary perfusion pressures 40–120 cmH2O. The hemimandibles were analysed radiographically (mm2 and pixels) to verify the presence of AP. Biomarkers of cardiac oxidative stress (OS) were determined in coronary venous effluent and cardiac tissue homogenate. Cardiac tissue was analysed histopathologically for signs of heart damage (oedema, fibrosis and necrosis). All data were analysed by Kruskal–Wallis and one‐way anova tests (p < .05). Results The levels of the maximum left ventricular pressure development rate of the SHR + AP group were significantly increased compared to the AP and C groups, and of the SHR group compared with the C group (p < .05). The levels of the minimum left ventricular pressure development rate of the SHR + AP group were significantly decreased compared to the AP, SHR and C groups, and of the SHR group compared to the C group (p < .05). The radiographic AP area was significantly larger in the SHR + AP group than in the AP group (p < .01). The levels of superoxide anion were significantly higher in the SHR + AP group than in the AP, SHR and C groups (p < .05). The activities of superoxide dismutase in cardiac tissue homogenate were significantly lower in the SHR + AP and AP groups compared with the SHR and C groups (p < .05). Conclusions In rats, AP was associated with impaired cardiodynamics, disturbed cardiac OS, antioxidant defence and cardiac pathologic changes in hypertensive conditions. Hypertension was associated with an increase in the AP radiographic area. Further studies should confirm whether root canal treatment can have a cardioprotective effect and reduce cardiac OS in hypertensive conditions.

show abstract

“…In the lysate, we determined the activity of non-enzymatic antioxidants, including reduced glutathione (GSH) and the activity of the enzymatic defense system by evaluating the catalase (CAT) and superoxide dismutase (SOD) levels, while in heart tissue homogenates, we determined CAT and SOD activity. Heart tissue homogenates were prepared according to a previously published method (Bradic et al, 2020). All mentioned biochemical parameters of oxidative stress were determined spectrophotometrically (Shimadzu UV-1800UV-VIS spectrophotometer, Japan).…”

Section: Oxidative Stress Markers and Antioxidant Enzyme Determinationmentioning

confidence: 99%

“…The degree of lipid peroxidation in the coronary venous effluent and plasma samples was estimated by measuring TBARS, using 1% thiobarbituric acid in 0.05 NaOH, which was incubated with the coronary effluent at 100 • C for 15 min and measured at 530 nm. Krebs-Henseleit solution was used as a blank probe (Bradic et al, 2020).…”

Section: Tbars Determination (Index Of Lipid Peroxidation)mentioning

confidence: 99%

“…A total of 0.5 ml of samples was precipitated with 200 µl of 30% sulpho-salicylic acid, vortexed for 30 min, and centrifuged at ×3,000 g. Equal volumes of the supernatant and Griess's reagent, containing 1% sulphanilamide in 5% phosphoric acid/0.1% naphthalene ethylenediamine-dihydrochloride were added and incubated for 10 min in the dark and measured at 543 nm. The nitrite levels were calculated using sodium nitrite as the standard (Bradic et al, 2020).…”

Section: Tbars Determination (Index Of Lipid Peroxidation)mentioning

confidence: 99%

See 1 more Smart Citation

New Insight Into the Cardioprotective Effects of Allium ursinum L. Extract Against Myocardial Ischemia-Reperfusion Injury

et al. 2021

Self Cite

View full text Add to dashboard Cite

This study aimed to estimate the effects of increasing doses of Allium ursinum methanol extract on cardiac ischemia/reperfusion injury (I/R) with a special emphasis on the role of oxidative stress. Fifty rats were randomly divided into five groups (10 animals per group) depending on the applied treatment as follows: sham, rats who drank only tap water for 28 days and hearts were retrogradely perfused for 80 min without I/R injury, I/R, rats who drank only tap water for 28 days and hearts were exposed to ex vivo I/R injury and rats who consumed increasing doses of A. ursinum 125, 250, and 500 mg/kg for 28 days before I/R injury. Hearts from all rats were isolated and retrogradely perfused according to the Langendorff technique. Parameters of oxidative stress were spectrophotometrically measured in blood, coronary venous effluent, and heart tissue samples. Intake of wild garlic extract for 28 days significantly contributed to the recovery of cardiac function, which was reflected through preserved cardiac contractility, systolic function, and coronary vasodilatory response after ischemia. Also, wild garlic extract showed the potential to modulate the systemic redox balance and stood out as a powerful antioxidant. The highest dose led to the most efficient decrease in cardiac oxidative stress and improve recovery of myocardial function after I/R injury. We might conclude that wild garlic possesses a significant role in cardioprotection and strong antioxidant activity, which implicates the possibility of its use alone in the prevention or as adjuvant antioxidant therapy in cardiovascular diseases (CVD).

show abstract

Cardioprotective effects of Galium verum L. extract against myocardial ischemia-reperfusion injury

Cited by 11 publications

References 20 publications

Morphine pretreatment reduces myocardial ischemiareperfusion injury in heart failure rats via GSK-3β/Cx43 signaling proteins and apoptosis-related gene, Bcl-2/Bax

Morphine pretreatment reduces myocardial ischemiareperfusion injury in heart failure rats via GSK-3β/Cx43 signaling proteins and apoptosis-related gene, Bcl-2/Bax

Cardiac, biochemical and histopathological analysis reveals impaired heart function in hypertensive rats with apical periodontitis

New Insight Into the Cardioprotective Effects of Allium ursinum L. Extract Against Myocardial Ischemia-Reperfusion Injury

Contact Info

Product

Resources

About