New Insight Into the Cardioprotective Effects of Allium ursinum L. Extract Against Myocardial Ischemia-Reperfusion Injury

Abstract

This study aimed to estimate the effects of increasing doses of Allium ursinum methanol extract on cardiac ischemia/reperfusion injury (I/R) with a special emphasis on the role of oxidative stress. Fifty rats were randomly divided into five groups (10 animals per group) depending on the applied treatment as follows: sham, rats who drank only tap water for 28 days and hearts were retrogradely perfused for 80 min without I/R injury, I/R, rats who drank only tap water for 28 days and hearts were exposed to ex vivo I/R injury and rats who consumed increasing doses of A. ursinum 125, 250, and 500 mg/kg for 28 days before I/R injury. Hearts from all rats were isolated and retrogradely perfused according to the Langendorff technique. Parameters of oxidative stress were spectrophotometrically measured in blood, coronary venous effluent, and heart tissue samples. Intake of wild garlic extract for 28 days significantly contributed to the recovery of cardiac function, which was reflected through preserved cardiac contractility, systolic function, and coronary vasodilatory response after ischemia. Also, wild garlic extract showed the potential to modulate the systemic redox balance and stood out as a powerful antioxidant. The highest dose led to the most efficient decrease in cardiac oxidative stress and improve recovery of myocardial function after I/R injury. We might conclude that wild garlic possesses a significant role in cardioprotection and strong antioxidant activity, which implicates the possibility of its use alone in the prevention or as adjuvant antioxidant therapy in cardiovascular diseases (CVD).

Antibacterial Potential of Allium ursinum Extract Prepared by the Green Extraction Method

“…In addition to the sulfur compounds, biomolecules such as peptides, flavonoids, phenols, alkaloids, and saponins contribute to the antimicrobial activity of Allium species or reveal synergistic effects with other present bioactive compounds [ 7 , 26 ]. Polyphenolic compounds have great structural diversity and variations in chemical composition and thus differ in their antibacterial effectiveness against pathogenic microorganisms [ 1 ].…”

“…Numerous studies support the fact that A. ursinum can be used as a natural antimicrobial agent. However, previous studies have shown conflicting results regarding the antimicrobial activity of A. ursinum extracts against various Gram-positive and Gram-negative bacteria [ 7 , 8 , 31 , 32 , 33 ]. As it was stated before, this can be explained by the isolation of different active compounds using different solvents during extraction, extraction method, plant origin and plant part.…”

“…A. ursinum has been used in traditional medicine for centuries. Several health benefits have been associated with A. ursinum : cardioprotective, antiplatelet, antidiabetic, antioxidative, antimicrobial, and anti-inflammatory [ 6 , 7 ]. These benefits have been attributed mainly to sulfur-containing compounds such as thiosulfinates and (poly)sulfides; however, the health benefits and strong antioxidant activity can also be attributed to the polyphenolic content [ 8 , 9 ].…”

Creatine phosphate administration in cardiac ischemia–reperfusion injury in rats: focus on differences between preconditioning, perconditioning, and postconditioning protocols

“…Parameters of heart function were recorded via a pressure transducer attached to a data acquisition system and displayed using the included software. Prior to performing experiments, the balloon was checked for filling capacity and transducer calibration was performed according to the manufacturer's instructions (Rankovic et al 2021).…”

“…The degree of lipid peroxidation in the coronary venous effluent was estimated by measuring TBARS, using 1% thiobarbituric acid in 0.05 M NaOH, which was incubated with the coronary effluent at 100 • C for 15 min and measured at 530 nm. The Krebs-Henseleit solution was used as a blank probe (Rankovic et al 2021).…”