Cardioprotective Effects of Fullerenol C₆₀(Oh)₂₄ on a Single Dose Doxorubicin-induced Cardiotoxicity in Rats with Malignant Neoplasm

Abstract

The therapeutic utility of the anthracycline antibiotic doxorubicin is limited due to its cardiotoxicity. Our aim was to investigate the efficacy of fullerenol C 60 (OH) 24 in preventing single, high-dose doxorubicin-induced cardiotoxicity in rats with malignant neoplasm. Experiment was performed on adult female Sprague Dawley rats with chemically induced mammary carcinomas. The animals were sacrificed two days after the application of doxorubicin and/or fullerenol, and the serum activities of CK, LDH and α-HBDH, as well as the levels of MDA, GSH, GSSG, GSH-Px, SOD, CAT, GR, and TAS in the heart, were determined. The results obtained from the enzymatic activity in the serum show that the administration of a single dose of 8 mg/kg in all treated groups induces statistically significant damage. There are significant changes in the enzymes of LDH and CK (p < 0.05), after an i.p. administration of doxorubicin/fullerenol and fullerenol. Comparing all groups with untreated control group, point to the conclusion that in the case of a lower α-HBDH/LDH ratio, results in more serious the liver parenchymal damage. The results revealed that doxorubicin induced oxidative damage and that the fullerenol antioxidative influence caused significant changes in MDA, GSH, GSSG, GSH-Px, SOD, CAT, GR, and TAS level in the heart (p < 0.05). Therefore, it is suggested that fullerenol might be a potential cardioprotector in doxorubicin-treated individuals.

Antioxidative nanofullerol prevents intervertebral disk degeneration

“…Among numerous reported fullerene derivatives, nanofullerol is the most extensively studied, and has been implicated as a powerful antioxidant in the biomedical field. [48][49][50][51][52][53][54][55][56] Therefore, fullerol was selected in our present study.…”

Antioxidant and Hepatoprotective Activity of Garlic Chives (Allium tuberosum) Ethanolic Extract on Doxorubicin-Induced Liver Injured Rats

“…Increased significant levels of SGOT without increased of SGPT levels are common in cardiac injury. [14] Malondialdehyde (MDA) is a metabolite of lipid peroxidation by free radicals. Malondialdehyde (MDA) formed when the free radical hydroxyl such as ROS reacts with fatty acid component of cell membrane.…”

Doxorubicin-Induced Oxidative Injury of Cardiomyocytes - Do We Have Right Strategies for Prevention?

“…8.). Again, it was confirmed oxidative nature of cardiac injury caused by anthracycline application (elevated parameters of oxidative stress -SOD, MDA, GST, GSH, GR and TAS) and antioxidative capability of fullerenol, which maintain all examinated parameters at the basal level, when is applied as pretreatment to doxorubicin (Injac, R. et al, 2008a) Protective effects of fullerenol C 60 (OH) 24 on Wistar rats with colorectal carcinoma (chronic regimen of DOX administration -1.5mg/kg/week, for 3 weeks)) was assessed by the same research group. They findings confirmed cardioprotective role of fullerenol, without modulation of antitumor activity of the drug.…”

“…8. Mitochondria showing (A) normal morphology, the double membrane envelope and lamellar cristae in the control rats; (B) irregular shape with lucent matrix and disorganised cristae in the Dox rats; and (C) amorphous material and rare disintegrated cristae in the Dox/Full rats (Injac, R. et al, 2008a).…”