Cardioprotective effect of Vedic Guard against doxorubicin-induced cardiotoxicity in rats: A biochemical, electrocardiographic, and histopathological study

Abstract

Background:Vedic Guard is a polyherbal formulation used in the treatment of various ailments, however, is not scientifically assessed for its effect on doxorubicin-induced cardiotoxicity.Objective:To find out the preventive role of Vedic Guard against doxorubicin-induced myocardial toxicity in rats.Materials and Methods:Cardiotoxicity was produced by doxorubicin (15 mg/kg for 2 weeks). Vedic Guard (270 mg/kg, orally) was administered as pre-treatment for 2 weeks and then for 2 weeks alternated with doxorubicin (DXR). The general observations, mortality, histopathology, biomarker like lactate dehydrogenase (LDH), creatine phosphokinase (CPK), aspartate aminotransferase (AST), alanine transaminase (ALT), electrocardiographic (ECG) parameters, antioxidants such as glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) were monitored after 3 weeks of last dose.Results:The repeated administration of DXR causes cardiomyopathy associated with an antioxidant deficit. Pre-treatment with Vedic Guard decreases serum enzyme viz LDH, CPK, AST, and ALT levels to that of normal values. Vedic Guard significantly protected the myocardium from the toxic effect of DXR, by increasing the levels of antioxidants such as GSH, SOD, and CAT and decreased the elevated level of malondialdehyde. The study shows significant alteration of ECG pattern in DXR administered rats. The characteristic findings were elevation of ST segment, reduction in P waves, QRS complex, and R-R interval. Vedic Guard showed a protective effect against DXR-induced altered ECG pattern. It also reduced the severity of cellular damage of the myocardium confirmed by histopathology.Conclusion:The results of the present study indicated cardioprotective effect of Vedic Guard might be attributed to its antioxidant activity.

Ameliorative effect of naringin against doxorubicin-induced acute cardiac toxicity in rats

“…It suggested that the biochemical anomalies might lead to cardiotoxicity. These present observations corroborated to the earlier experimental reports (Koti et al, 2013;Mantawy et al, 2014;Matouk et al, 2013;Mukherjee et al, 2003;Singh et al, 2012). Cardiomyocytes are more susceptible to Dox-induced damage than other tissues due to highly active metabolic machinery in heart (Nohl et al, 1998;Zhang et al, 2009).…”

Oxymatrine Ameliorates Doxorubicin-Induced Cardiotoxicity in Rats

“…CAT, MDA, SOD and GSH-Px are important markers of oxidative stress and are widely regarded as indicators of oxidative injury [23, 24]. We measured levels of these markers to test whether pre-treatment with oxymatrine improved doxorubicin-induced metabolic derangements in rat myocardium.…”

Combinatorial resveratrol and quercetin polymeric micelles mitigate doxorubicin induced cardiotoxicity in vitro and in vivo

“…[22] Therefore, we designed the animal study to induce acute cardiotoxicity by dosing at 18 mg/kg over 10 days and evaluated the cardioprotective effect of the mRQ dosed concurrently.…”