Cardioprotective Effect of Rheum turkestanicum Against Doxorubicin-Induced Toxicity in Rats

Abstract

Background: Doxorubicin as an anti-cancer drug causes cardiotoxicity, limiting its tolerability and use. The mechanism of toxicity is due to free radical production and cardiomyocytes injury. This research evaluated Rheum turkestanicum (R.turkestanicum) extract against doxorubicin cardiotoxicity due to its considerable in vitro antioxidant activity.Methods: Male Wistar rats received 2.5 mg/kg doxorubicin intraperitoneally every other day for 2 weeks to create an accumulative dose. R. turkestanicum was administrated at a dose of 100 and 300 mg/kg intraperitoneally from the second week for 7 days. On the 15th day, the animals were anesthetized and blood was collected from cardiac tissue for evaluation of alanine aminotransferase (ALT), cardiac muscle creatinine kinase (CK-MB), troponin T (cTn-T), lactate dehydrogenase (LDH), and B-type natriuretic peptide brain natriuretic peptide. A cardiac homogenate was also collected to determine superoxide dismutase (SOD), catalase Catalase Activity, malondialdehyde (MDA), and thiols. Histopathology was also performed.Results: Doxorubicin increased all cardiac enzymes and malondialdehyde, correlating with a reduction in SOD, catalase, and thiols. Histopathology revealed extracellular edema, moderate congestion, and hemorrhage of foci. In contrast, administration of R. turkestanicum ameliorated these doxorubicin-induced pathophysiological changes.Conclusion: This study revealed that the extract ameliorated doxorubicin-induced cardiac toxicity via modulation of oxidative stress-related pathways. Liquid chromatography-mass spectrometry analysis of R. turkestanicum indicated several components with potent pharmacological properties.

Cardioprotective effect of Sanguisorba minor against isoprenaline-induced myocardial infarction in rats

“…Approximately, a third of all drugs approved by the Food and Drug Administration (FDA) over the past 20 years have been obtained from or inspired by natural/herbal compounds/derivatives (Izzo et al, 2020). In traditional medicine, there are several natural products such as extracts, herbomineral, polyherbal formulations, and crude herbals, which can be used for CVD Anwar et al, 2018;Hosseini et al, 2022). For example, the cardioprotective activities of some phytochemicals such as silymarin, quercetin, curcumin, berberine, naringenin, baicalin, resveratrol, and myricitrin have been well documented in various studies (Rao and Viswanath, 2007;Giannouli et al, 2018;Hesari et al, 2021).…”

Mercuric chloride‐induced oxidative stress, genotoxicity, haematological changes and histopathological alterations in fish Channa punctatus (Bloch, 1793)

“…Hosseini et al . (2018) have also reported hepatic and renal histopathological damage in rats intoxicated with HgCl 2 . Similar histopathological changes were observed in the liver of fish Danio rerio exposed to HgCl 2 (Macirella et al ., 2016).…”

Etlingera hemisphaerica Blume attenuates male reproductive toxicity due to mercury chloride in Mus musculus

“…HgCl 2 signi cantly raises liver MDA levels, decreases total sulfhydryl content, and increases serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity. The liver functions were enhanced by the R. turkestanicum extract, as evidenced by signi cant reductions in AST and ALT levels in serum, decreased MDA, and increased total sulfhydryl content [47].…”