Cardiac functional improvement by a human Bcl-2 transgene in a mouse model of ischemia/reperfusion injury

Brocheriou, Valérie; Hagège, Albert; Oubenaissa, Abdeslam; Lambert, M.; Mallet, Vincent; Duriez, Micheline; Wassef, Michel; Kahn, Axel; Menasché, Philippe; Gilgenkrantz, Hélène

doi:10.1002/1521-2254(200009/10)2:5<326::aid-jgm133>3.0.co;2-1

Cited by 166 publications

(101 citation statements)

References 28 publications

(33 reference statements)

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“…The present results confirmed this report and demonstrated that a moderate hypercholesterolemia (2% cholesterol diet for 8 weeks) also increased myocardial apoptosis. Several studies stated that inhibition of apoptosis by a variety of pharmacological and genetic approaches results in smaller infarction (Brocheriou et al, 2000;Mocanu et al, 2001) and improved cardiac function (Abbate et al, 2002;Chatterjee et al, 2003). Taurine, is known as to protect various disorders and has been demonstrated to have beneficial effects in nephropathy, cardiomyopathy and neuropathy, (Szymanski and Winiarska, 2008).…”

Section: Discussionmentioning

confidence: 99%

Taurine Attenuates Hepatic and Cardiac Damage and Apoptosis in Rabbits Fed a High-Fat Diet

Abdel-Rahman¹

2014

OnLine Journal of Biological Sciences

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A hypercholesterolemia diet has been associated with the hepatic and cardiac abnormalities and the pathological changes. The present work was designed to investigate the histological and immunohistochemical changes in the liver and heart of rabbits when fed high fat diet and the possible protective role of an antioxidant "taurine". Twenty-four male white New Zealand rabbits were divided into four groups, 6 rabbits each. Group 1, served as a control, rabbits fed with a normal diet. Group 2, (taurine group), rabbits were given orally taurine (10 mg Kg −1 b.w/day) for 8 weeks. Group 3 (hypercholesterolemic group), rabbits fed (2% cholesterol-enriched diet for 8 weeks. Group 4, rabbits fed 2% cholesterol-enriched diet plus taurine (10 mg Kg −1 b.w/day) orally for the same period. Histopathological examinations revealed that high cholesterol diet caused hepatic and myocardial tissue changes compared with rabbits fed with a normal diet. Including fatty degeneration, inflammations and necrosis of Hepatocytes and vacuolar degeneration, disorganization of myofibrils and necrosis of myocardial cells. Immunohistochemistry for caspase-3 for apoptosis were performed. Caspase-3 positive cells in the liver tissue and Caspase-3 positive area in myocardial tissue increased in high cholesterol diet group. Taurine markedly attenuate hypercholesterolemia-induced cardiac and hepatic histopathological changes in the cholesterol plus taurine group compared to the cholesterol group. Thus, the results suggest that taurine could play a beneficial role against hypercholesterolemiainduced complications in the liver and heart of the rabbits.

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Section: Discussionmentioning

confidence: 99%

Taurine Attenuates Hepatic and Cardiac Damage and Apoptosis in Rabbits Fed a High-Fat Diet

Abdel-Rahman¹

2014

OnLine Journal of Biological Sciences

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“…Among them, both inhibiting apoptosis protein Bcl-2 and promoting apoptosis protein Bax have been extensively studied at present, but their function is still unclear. Brocheriou et al [5] reported a signifi cant reduction of infarct size and cardiomyocyte apoptosis in transgenic mice after pmyocardial ischemia-reperfusion, overexpressing anti-apoptotic human Bcl-2 in cardiomyocyte. In transgenic mice with human Bcl-2 transcripts other than in nontransgenic mice with myocardial ischemia-reperfusion, Chen et al [6] found a significant functional recovery of the heart, small infarct sizes (52.4%), and few apoptotic positive myocytes, accompanied by a three-fold decrease in lactate dehydrogenase (LDH).…”

Section: Discussionmentioning

confidence: 99%

Effect of exogenous phosphocreatine on cardiomycytic apoptosis and expression of Bcl-2 and Bax after cardiopulmonary resuscitation in rats

Yan

Chen

et al. 2011

World Journal of Emergency Medicine

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“…It was demonstrated that genetic modification of the myocardium with the anti-apoptotic human Bcl-2 gene conferred myocardial protection against ischemia/reperfusion-induced apoptosis [34,35]. Furthermore, several studies have reported a reduction of apoptosis by ischemic preconditioning via altering the expression of Bcl-2 and/or Bax protein, thus increased the ratio of Bcl-2/Bax protein in preventing the progression of apoptosis in myocardium after ischemia and reperfusion [20,21].…”

Section: Discussionmentioning

confidence: 99%

Intermittent hypoxia attenuates ischemia/reperfusion induced apoptosis in cardiac myocytes via regulating Bcl-2/Bax expression

et al. 2003

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Intermittent hypoxia has been shown to provide myocardial protection against ishemia/reperfusion-induced injury. Cardiac myocyte loss through apoptosis has been reported in ischemia/reperfusion injury. Our aim was to investigate whether intermittent hypoxia could attenuate ischemia/reperfusion-induced apoptosis in cardiac myocytes and its potential mechanisms. Adult male Sprague-Dawley rats were exposed to hypoxia simulated 5000 m in a hypobaric chamber for 6 h/day, lasting 42 days. Normoxia group rats were kept under normoxic conditions. Isolated perfused hearts from both groups were subjected to 30 min of global ischemia followed by 60 min reperfusion. Incidence of apoptosis in cardiac myocytes was determined by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) and DNA agarose gel electrophoresis. Expressions of apoptosis related proteins, Bax and Bcl-2, in cytosolic and membrane fraction were detected by Western Blotting. After ischemia/reperfusion, enhanced recovery of cardiac function was observed in intermittent hypoxia hearts compared with normoxia group. Ischemia/ reperfusion-induced apoptosis, as evidenced by TUNEL-positive nuclei and DNA fragmentation, was significantly reduced in intermittent hypoxia group compared with normoxia group. After ischemia/reperfusion, expression of Bax in both cytosolic and membrane fractions was decreased in intermittent hypoxia hearts compared with normoxia group. Although ischemia/reperfusion did not induce changes in the level of Bcl-2 expression in cytosolic fraction between intermittent hypoxia and normoxia groups, the expression of Bcl-2 in membrane fraction was upregulated in intermittent hypoxia group compared with normoxia group. These results indicated that the cardioprotection of intermittent hypoxia against ischemia/reperfusion injury appears to be in part due to reduce myocardial apoptosis. Intermittent hypoxia attenuated ischemia/reperfusion-induced apoptosis via increasing the ratio of Bcl-2/Bax, especially in membrane fraction.

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Cardiac functional improvement by a human Bcl-2 transgene in a mouse model of ischemia/reperfusion injury

Cited by 166 publications

References 28 publications

Taurine Attenuates Hepatic and Cardiac Damage and Apoptosis in Rabbits Fed a High-Fat Diet

Taurine Attenuates Hepatic and Cardiac Damage and Apoptosis in Rabbits Fed a High-Fat Diet

Effect of exogenous phosphocreatine on cardiomycytic apoptosis and expression of Bcl-2 and Bax after cardiopulmonary resuscitation in rats

Intermittent hypoxia attenuates ischemia/reperfusion induced apoptosis in cardiac myocytes via regulating Bcl-2/Bax expression

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