Cardiac cell–integrated microneedle patch for treating myocardial infarction

Tang, Junnan; Wang, Jinqiang; Huang, Ke; Ye, Yanqi; Su, Teng; Qiao, Li; Hensley, Michael Taylor; Caranasos, Thomas G.; Zhang, Jinying; Gu, Zhen

doi:10.1126/sciadv.aat9365

Cited by 208 publications

(176 citation statements)

References 53 publications

(65 reference statements)

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“…Acute myocardial infarctions (AMIs), principally caused by the occlusion of a coronary artery, are a major cause of death and disability worldwide (1). MIs induce the reduction of blood flow to heart muscles and result in myocardial necrosis (2,3). The myocardial necrosis triggers a sterile inflammatory response that contributes to adverse left ventricular (LV) remodeling and heart failure (4)(5)(6).…”

Section: Introductionmentioning

confidence: 99%

Targeted anti–IL-1β platelet microparticles for cardiac detoxing and repair

Huang

et al. 2020

Sci. Adv.

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An acute myocardial infarction (AMI) induces a sterile inflammatory response that facilitates further heart injury and promotes adverse cardiac remodeling. Interleukin-1β (IL-1β) plays a central role in the sterile inflammatory response that results from AMI. Thus, IL-1β blockage is a promising strategy for treatment of AMI. However, conventional IL-1β blockers lack targeting specificity. This increases the risk of serious side effects. To address this problem herein, we fabricated platelet microparticles (PMs) armed with anti–IL-1β antibodies to neutralize IL-1β after AMI and to prevent adverse cardiac remodeling. Our results indicate that the infarct-targeting PMs could bind to the injured heart, increasing the number of anti–IL-1β antibodies therein. The anti–IL-1β platelet PMs (IL1-PMs) protect the cardiomyocytes from apoptosis by neutralizing IL-1β and decreasing IL-1β–driven caspase-3 activity. Our findings indicate that IL1-PM is a promising cardiac detoxification agent that removes cytotoxic IL-1β during AMI and induces therapeutic cardiac repair.

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Section: Introductionmentioning

confidence: 99%

Targeted anti–IL-1β platelet microparticles for cardiac detoxing and repair

Huang

et al. 2020

Sci. Adv.

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“…Thus, much effort has been devoted to ISF biomarker detecting, such as microchips, microneedle arrays (MNs) and so on. Among them, MNs are one of the most effective and secure methods, owing to their ability to penetrate the skin and form microscale passages without contacting nerve fibers and capillaries . By this means, numerous ISF biomarkers could be extracted, accumulated, and analyzed accurately in vitro .…”

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confidence: 99%

Encoded Microneedle Arrays for Detection of Skin Interstitial Fluid Biomarkers

et al. 2019

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Skin interstitial fluid (ISF) is considered as an emerging source of biomarkers with physiological and medical significance. Microneedle arrays (MNs) provide a promising means for painless, noninvasive detection of these biomarkers. Here, novel MNs integrated with photonic crystal (PhC) barcodes are presented, and multiplex specific detection of ISF biomarkers is realized for the first time. The PhC barcodes‐loaded flexible MNs are simply fabricated by replicating dynamic ferrofluid‐cast micromoldings. When the prepared MNs are inserted into skin, they can enrich specific biomarkers to their probes‐decorated PhC barcodes. Thus, by adding corresponding fluorescent probes to form sandwich immunocomplexes, the relative content of the biomarkers can be read out through the fluorescence intensity of the barcodes; meanwhile, the species of these biomarkers can be clearly distinguished by the reflection peaks of the PhC barcodes. Based on the encoded MNs, their sensitivity, flexibility, and versatility of capturing and detecting three inflammatory cytokines are demonstrated in a sepsis mice model. Compared with existing MNs for ISF detection, the encoded MNs not only possess equivalent detection effects with less post‐processing and simplified procedures, but can also detect multiple biomarkers simultaneously, which makes them ideal in many clinical and biomedical detection areas.

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“…To date gene therapy for heart failure by using AAV gene vectors are currently into clinical trials [99,100]. Epicardial patches, currently used to provide exogenous cardiac cells toward the injured site [101,102], may also represent an interesting option to administrate paracrine factors known to target key regulators of cardiomyocyte proliferation [103]. This review highlights the crucial role of diverse mechanisms including cell cycle regulators, Hippo pathway, oxygen exposure, energy metabolism, ECM and growth factors in the regulation of cardiomyocyte proliferation and cardiac regeneration.…”

Section: Discussionmentioning

confidence: 99%

Cardiomyocyte proliferation, a target for cardiac regeneration

Payan

Hubert

Rochais

2020

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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Cardiac diseases, characterized by cardiomyocyte loss, lead to dramatic impairment of cardiac function and ultimately to congestive heart failure. Despite significant advances, conventional treatments do not correct the defects in cardiac muscle cell numbers and the prognosis of congestive heart failure remains poor. The existence, in adult mammalian heart, of low but detectable cardiomyocyte proliferative capacities has shifted the target of regenerative therapy toward new therapeutical strategy. Indeed, the stimulation of terminally differentiated cardiomyocyte proliferation represents the main therapeutic approach for heart regeneration. Increasing evidence demonstrating that the loss of mammalian cardiomyocyte renewal potential shortly after birth causes the loss of regenerative capacities, strongly support the hypothesis that a detailed understanding of the molecular mechanisms controlling fetal and postnatal cardiomyocyte proliferation is essential to identify targets for cardiac regeneration. Here, we will review major developmental mechanisms regulating fetal cardiomyocyte proliferation and will describe the impact of the developmental switch, operating at birth and driving postnatal heart maturation, on the regulation of adult cardiomyocyte proliferation, all these mechanisms representing potential targets for cardiac repair and regeneration.

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Cardiac cell–integrated microneedle patch for treating myocardial infarction

Abstract: A microneedle cardiac stromal cell patch has been developed for therapeutic heart regeneration after myocardial infarction.

Cited by 208 publications

References 53 publications

Targeted anti–IL-1β platelet microparticles for cardiac detoxing and repair

Targeted anti–IL-1β platelet microparticles for cardiac detoxing and repair

Encoded Microneedle Arrays for Detection of Skin Interstitial Fluid Biomarkers

Cardiomyocyte proliferation, a target for cardiac regeneration

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