Carcinoembryonic antigen production by human colorectal adenocarcinoma cells in matrix-perfusion culture

Quarles, John; Morris, Nell G.; Leibovitz, Albert

doi:10.1007/bf02831502

Cited by 25 publications

(8 citation statements)

References 12 publications

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“…L929 cells were expanded by standard cell culture techniques [25][26][27] in 75 cm 2 tissue culture asks (3110-075, Iwaki Glass, Tokyo, Japan) containing 40 ml of 10% serum-supplemented medium in a CO 2 incubator (BNA-111, Espec, Osaka, Japan) in 5% CO 2 atmosphere at 37 ± C. The cell lines were used between passage numbers 20 to 55 in the following experiments. After UV irradiation (30 cm distance, 10 W, GL10, Stanley Co., Tokyo, Japan) of the cast and LB lms for 20 min for sterilization, the lms were inserted into 24-well tissue culture plates tted with a lid (Iwaki Glass, Tokyo, well diameter D 16 mm).…”

Section: Cell Culture On Polymeric Lmsmentioning

confidence: 99%

Growth of L929 cells on polymeric films prepared by Langmuir–Blodgett and casting methods

Higuchi

Tamiya

Tsubomura

et al. 2000

Journal of Biomaterials Science, Polymer Edition

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The growth and spreading of fibroblast, L929 cells, on various polymeric films prepared by the Langmuir-Blodgett (LB) and casting methods were investigated. L929 cells, which were cultivated on collagen and synthetic polymeric films prepared by the LB method, adhered and spread much more than those on synthetic films prepared by the casting method. This is explained by the fact that cell growth and cell spreading are suitable for L929 cells on the films having serum proteins that contain a high alpha-helix content, because LB films adsorbed those serum proteins estimated from the circular dichroism measurements of the films immersed in cell culture medium. An exponential relationship was observed from the plot of the cell density vs root mean square of roughness of the films, which is estimated by atomic force microscopy, whereas a linear relationship was observed from the plot of the spreading ratio vs the root mean square of roughness. It is suggested that the correlation between the cell growth or spreading ratio and surface roughness of the films where L929 cells were cultivated is considered to be more important than the correlation between the cell growth or spreading ratio and the contact angle of the films.

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Section: Cell Culture On Polymeric Lmsmentioning

confidence: 99%

Growth of L929 cells on polymeric films prepared by Langmuir–Blodgett and casting methods

Higuchi

Tamiya

Tsubomura

et al. 2000

Journal of Biomaterials Science, Polymer Edition

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“…Most hollow-fiber bioreactors are similar in design and size to modules used in hemodialysis of uremic patients (6). Hollow-fiber bioreactors are used for production of cell-derived products such as antibodies (7), cytokines (8), and specific tumor-associated antigens (9) and for the expansion of activated lymphocytes (10,11). However, most commercially available hollow-fiber bioreactors represent a large scale approach.…”

Section: Introductionmentioning

confidence: 99%

New Miniaturized Hollow-Fiber Bioreactor for in Vivo Like Cell Culture, Cell Expansion, and Production of Cell-Derived Products

Gloeckner¹,

Lemke²

2001

Biotechnol. Prog.

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We have developed a miniaturized hollow-fiber bioreactor system for mammalian cell culture with a volume of 1 mL. Cell and medium compartments of the bioreactor are separated by a semipermeable membrane, and oxygenation of the cell compartment is accomplished using an oxygenation membrane. As a result of the geometry of the transparent housing, cells can be observed by microscopy during culture. The leukemic cell lines CCRF-CEM, HL-60, and REH were cultivated up to densities of 3.5 x 10(7)/mL without medium change or manipulation of the cells. As shown using CCRF-CEM cells, growth in the bioreactor was strongly influenced and could be controlled by the medium flow rate. As a consequence, consumption of glucose and generation of lactate varied with flow rate. Depending on the molecular size cutoff of the membranes used, added growth factors such as GM-CSF, as well as factors secreted from the cells, are retained in the cell compartment for up to 1 week. This new miniaturized hollow-fiber bioreactor offers advantages in tissue engineering by continuous nutrient supply for cells in high density, retention of added or autocrine produced factors, and undisturbed long-term culture in a closed system.

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“…(14,16,17). To compare the CEA production rate between different cell lines, CEA production rates obtained in this study and cited from the literature (12,14,15) are summarized in Table 1. CW2 cells can produce 60 ag after incubation for 34 …”

Section: Introductionmentioning

confidence: 99%

Hollow Fiber Bioreactor for High Density and Continuous Production of Carcinoembryonic Antigen.

et al. 1998

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: Continuous cultivation of CW2 cells (a human colorectal adenocarcinoma tumor cell) using a hollow fiber bioreactor was investigated for the production of carcinoembryonic antigen (CEA) over 34 days. The CEA production rate increased with the increase in incubation time up to 20 days and was found to be approximately a constant value, 20 ng/cm2 day, after the incubation time of 20 days. The CEA production rate was known to vary in different cell lines. The CEA production rates were compared with those obtained in this study and in the literature using hollow fiber bioreactors. It was found that CW2 cells produced CEA moderately among those cell lines. The high cell density (9.1 X 10' cells/cmz) achieved in the hollow fiber bioreactor compared to the flask culture (4.0 X 105 cells/cm2) made it possible to obtain continuous high production of CEA over an extended time period.

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Carcinoembryonic antigen production by human colorectal adenocarcinoma cells in matrix-perfusion culture

Cited by 25 publications

References 12 publications

Growth of L929 cells on polymeric films prepared by Langmuir–Blodgett and casting methods

Growth of L929 cells on polymeric films prepared by Langmuir–Blodgett and casting methods

New Miniaturized Hollow-Fiber Bioreactor for in Vivo Like Cell Culture, Cell Expansion, and Production of Cell-Derived Products

Hollow Fiber Bioreactor for High Density and Continuous Production of Carcinoembryonic Antigen.

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