Carboxymethyl chitosan as a polyampholyte mediating intrafibrillar mineralization of collagen via collagen/ACP self-assembly

Lin, Mingli; Liu, Huanhuan; Deng, Jingjing; An, Ran; Shen, Minjuan; Li, Yanqiu; Zhao-yu, XU

doi:10.1016/j.jmst.2019.05.010

Cited by 30 publications

(10 citation statements)

References 45 publications

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“…The results mentioned before prove that the combined application of bioskiving and adequate sonication treatment is a feasible approach for preparing the porous collagen membrane. Recently, synchronous self-assembly/mineralization (SSM) is an emerging novel approach to fabricate mineralized porous collagen membranes ( Lin et al, 2019 ; Liu et al, 2020 ). In brief, collagen molecules and amorphous mineral nanoparticles stabilized by the polyampholytes were mixed in the acidic solution.…”

Section: Discussionmentioning

confidence: 99%

A Rapid and Convenient Approach to Construct Porous Collagen Membranes via Bioskiving and Sonication-Feasible for Mineralization to Induce Bone Regeneration

Zhong²,

Yu³

et al. 2021

Front. Bioeng. Biotechnol.

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Porous mineralized collagen membranes efficiently promote bone regeneration. To generate them, we need to fabricate collagen membranes that are porous. However, the current fabrication method is primarily based on a bottom-up strategy, with certain limitations, such as a long manufacturing process, collagen denaturation, and failure to control fibril orientation. Using a top-down approach, we explore a novel method for constructing porous collagen membranes via the combined application of bioskiving and sonication. Numerous collagen membranes with well-aligned fibril structures were rapidly fabricated by bioskiving and then sonicated at 30, 60, 90, and 120 W for 20 min. This treatment allowed us to study the effect of power intensity on the physicochemical traits of collagen membranes. Subsequently, the prepared collagen membranes were immersed in amorphous calcium phosphate to evaluate the feasibility of mineralization. Additionally, the bioactivities of the membranes were assessed using preosteoblast cells. Tuning the power intensity was shown to modulate fibril orientation, and the porous membrane without denatured collagen could be obtained by a 20-min sonication treatment at 90 W. The prepared collagen membrane could also be further mineralized to enhance osteogenesis. Overall, this study offers a rapid and convenient approach for fabricating porous collagen membranes via bioskiving and sonication.

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Section: Discussionmentioning

confidence: 99%

A Rapid and Convenient Approach to Construct Porous Collagen Membranes via Bioskiving and Sonication-Feasible for Mineralization to Induce Bone Regeneration

Zhong²,

Yu³

et al. 2021

Front. Bioeng. Biotechnol.

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“…Accordingly, the swelling capacity of the CMCScontaining layer is far inferior to that of the CMC-containing one. On the other hand, the isoelectric point of CMCS was in the acidic range (pH = 3.5−5.5), 61,62 which was also not conducive to the swelling of the CMCS-containing layer in acidic medium. As a result, the bilayer bent toward the CMCScontaining side.…”

Section: Bilayer Hydrogels As a Ph-responsive Actuatormentioning

confidence: 99%

Heterogeneity Regulation of Bilayer Polysaccharide Hydrogels for Integrating pH- and Humidity-Responsive Actuators and Sensors

Zhang

Jiang

2023

ACS Appl. Mater. Interfaces

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Bilayer hydrogel-based actuators have attracted much interest because inhomogeneous structures are easily constructed in hydrogels. We used three kinds of polysaccharides, including anionic carboxymethyl cellulose (CMC), cationic chitosan (CS), and amphoteric carboxymethyl chitosan (CMCS), as both structure-constructing units and actuation-controlling units in this work to fabricate physically crosslinked poly(vinyl alcohol) bilayer hydrogels. The spatial heterogeneity was tuned by changing the types and concentrations of polysaccharides in different layers, to regulate pH-and humidity-driven actions of bilayer hydrogels. Based on the distortion of the ionic channel during the humidity-motivated deformation of bilayer hydrogels, a two-inone flexible device integrating a humidity-driven actuator and humidity-responsive sensor was then developed, which could detect the alterations of environmental humidity in real time. Moreover, good tensile toughness and interfacial bonding as well as the strain−resistance effect endowed the bilayer hydrogels with the capability of identifying human motion as a strain sensor, unlocking more application scenarios. This work provides an overall insight into the heterogeneity regulation of bilayer hydrogels using polysaccharides as stimulus-responsive units and also proposes an interesting strategy of manufacturing hydrogel-based flexible devices with both actuating and sensing capabilities.

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“…The occurrence of a sequence of dissolution and recrystallization steps followed by the formation of less stable intermediates from this metastable amorphous phase results in the crystallization of biological apatite, which gives rise to the bone's building blocks. Adapted from Lin et al 31 hybrid bone grafts as well as to understand how mineralizing cells generate and interact with the ECM during bones formation, which is a major gap in bone-cell biology. 2,4,11 Therefore, 3D bone models more closely resembling human physiological bone would gain benefit from the research on the structure-function relationship of the bone.…”

Section: The Formation Of the Bone's Inorganic Phasementioning

confidence: 99%

Three‐dimensional cell‐laden collagen scaffolds: From biochemistry to bone bioengineering

et al. 2021

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The bones can be viewed as both an organ and a material. As an organ, the bones give structure to the body, facilitate skeletal movement, and provide protection to internal organs. As a material, the bones consist of a hybrid organic/inorganic threedimensional (3D) matrix, composed mainly of collagen, noncollagenous proteins, and a calcium phosphate mineral phase, which is formed and regulated by the orchestrated action of a complex array of cells including chondrocytes, osteoblasts, osteocytes, and osteoclasts. The interactions between cells, proteins, and minerals are essential for the bone functions under physiological loading conditions, trauma, and fractures. The organization of the bone's organic and inorganic phases stands out for its mechanical and biological properties and has inspired materials research. The objective of this review is to fill the gaps between the physical and biological characteristics that must be achieved to fabricate scaffolds for bone tissue engineering with enhanced performance. We describe the organization of bone tissue highlighting the characteristics that have inspired the development of 3D cell-laden collagenous scaffolds aimed at replicating the mechanical and biological properties of bone after implantation. The role of noncollagenous macromolecules in the organization of the collagenous matrix and mineralization ability of entrapped cells has also been reviewed. Understanding the modulation of cell activity by the extracellular matrix will ultimately help to improve the biological performance of 3D cell-laden collagenous scaffolds used for bone regeneration and repair as well as for in vitro studies aimed at unravelling physiological and pathological processes occurring in the bone.

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Carboxymethyl chitosan as a polyampholyte mediating intrafibrillar mineralization of collagen via collagen/ACP self-assembly

Cited by 30 publications

References 45 publications

A Rapid and Convenient Approach to Construct Porous Collagen Membranes via Bioskiving and Sonication-Feasible for Mineralization to Induce Bone Regeneration

A Rapid and Convenient Approach to Construct Porous Collagen Membranes via Bioskiving and Sonication-Feasible for Mineralization to Induce Bone Regeneration

Heterogeneity Regulation of Bilayer Polysaccharide Hydrogels for Integrating pH- and Humidity-Responsive Actuators and Sensors

Three‐dimensional cell‐laden collagen scaffolds: From biochemistry to bone bioengineering

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