Carboxyl-functionalized graphene oxide composites as SPR biosensors with enhanced sensitivity for immunoaffinity detection

Chiu, Nan Fu; Fan, Shi Yuan; Yang, Cheng; Huang, Teng Yi

doi:10.1016/j.bios.2016.06.073

Cited by 139 publications

(65 citation statements)

References 49 publications

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“…The development of new sensing materials has received great attention, and especially graphene-like two-dimensional (2D) layered nanomaterials such as graphene (Singh et al, 2015;Chiu et al, 2018b), graphene oxide (GO) (Chiu and Huang, 2014;Stebunov et al, 2015;Chiu et al, 2017a), molybdenum disulfide (MoS 2 ) (Lee et al, 2014;Zhang et al, 2015), and functionalized-GO (Chiu et al, 2017b) and functionalized-MoS 2 (Chiu and Lin, 2018) nanocomposites. We successfully demonstrated that the modified carboxyl film could enhance sensing sensitivity.…”

Section: Introductionmentioning

confidence: 99%

High-Sensitivity Detection of the Lung Cancer Biomarker CYFRA21-1 in Serum Samples Using a Carboxyl-MoS2 Functional Film for SPR-Based Immunosensors

Chiu

Yang

2020

Front. Bioeng. Biotechnol.

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We constructed a novel surface plasmon resonance (SPR) detection assay using carboxyl-functionalized molybdenum disulfide (carboxyl-MoS 2) nanocomposites as a signal amplification sensing film for the ultrasensitive detection of the lung cancer-associated biomarker cytokeratin 19 fragment (CYFRA21-1). The experiment succeeded in MoS 2 reacted with chloroacetic acid giving carboxyl-MoS 2 as the reaction product. The additional shoulder in the C 1s and O 1s peaks of carboxyl-MoS 2 , which were increased in X-ray photoelectron spectroscopy, confirmed the presence of O-C=O groups on the surface of the carboxyl-MoS 2. Compared to MoS 2 , the experimental results confirmed that carboxyl-modified MoS 2 had improved low impedance and low refractive index. The carboxyl-MoS 2-based chip had a high affinity, with an SPR angle shift enhanced by 2.6-fold and affinity binding K A enhanced by 15-fold compared to a traditional SPR sensor. The results revealed that the carboxyl-MoS 2-based chip had high sensitivity, specificity, and SPR signal affinity, while the CYFRA21-1 assay in spiked clinical serum showed a lower detection limit of 0.05 pg/mL and a wider quantitation range (0.05 pg/mL to 100 ng/mL). The carboxyl-MoS 2-based chip detection value was about 10 4 times more sensitive than the limit of detection of an enzyme-linked immunosorbent assay (ELISA) (0.60 ng/mL). The results showed that the carboxyl-MoS 2-based chip had the potential to rapidly assay complex samples including bodily fluids, whole blood, serum, plasma, urine, and saliva in SPR-based immunosensors to diagnose diseases including cancer.

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Section: Introductionmentioning

confidence: 99%

High-Sensitivity Detection of the Lung Cancer Biomarker CYFRA21-1 in Serum Samples Using a Carboxyl-MoS2 Functional Film for SPR-Based Immunosensors

Chiu

Yang

2020

Front. Bioeng. Biotechnol.

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“…The functionalization of graphene or graphene oxide (GO) nanosheets confers specific properties to the composites, e.g., their chemical selectiveness, solubility, thermal and electronic conductivity (Bueno et al, 2017;Xiang et al, 2016). Such enhancement allows the employment of functionalized graphenebased materials in several fields, such as chemistry and catalysis (Ye et al, 2018;Rana and Jonnalagadda, 2017), biomedicine (Kenry et al, 2018;Yu et al, 2018;Zeng et al, 2017), electronics (Scidà et al, 2018;Gevaerd et al, 2018;Chiu et al, 2017;Zhao et al, 2017), energy (Li and Zhi, 2018;Sadri et al, 2017) and environmental sciences and technologies (Ren et al, 2018;Othman et al, 2018;Khurana et al, 2018). Due to their large specific surface area and rich porous structure, carbonaceous materials usually show high adsorption capacity and have been successfully employed as adsorbents of organic pollutants .…”

Section: Introductionmentioning

confidence: 99%

Functionalized Graphene-Based Materials as Innovative Adsorbents of Organic Pollutants: A Concise Overview

Fraga

Carvalho

Ghislandi

et al. 2019

Braz. J. Chem. Eng.

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The functionalization of graphene nanosheets is the cutting edge of materials sciences nowadays. Such research promotes the development of innovative, low cost and highly capable sorbents. This review article aims to assemble the available information on functionalized graphene used for the adsorption of organic pollutants and establishes a critical comparison between the data reported in the literature. Various optimal experimental conditions (pH, temperature, contact time, adsorbent dosage) and adsorbent characterization methods (FTIR, Raman, XPS spectra, XRD, TEM and AFM) have been listed to enlighten adsorption mechanisms, capacity and limiting aspects. Moreover, adsorption isotherms, kinetics and thermodynamic data of different functionalized graphene-based materials towards a wide range of organic pollutants were analyzed and tabulated. In each evaluation topic, environmental and human health protection is subject for discussion, as well as the scientific breakthrough works available in high impact journals in the field.

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“…[33][34][35][36][37]. Although CG was widely applied in semiconductors [38,39], supercapacitors [40], electrochemiluminescence [41], biosensors [42][43][44], and hydrogels [45], less literature is available on its application in membrane separation comparing with GO. To the best of our knowledge, Gao et al [46,47] prepared CG and CG/polyamide nanofiltration membranes and applied them for dye solution desalination.…”

Section: Introductionmentioning

confidence: 99%

Poly(vinyl alcohol)/Carboxyl Graphene Membranes for Ethanol Dehydration by Pervaporation

et al. 2020

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Carboxyl graphene (CG) with two functions of hybridization and crosslinking was incorporated into poly(vinyl alcohol) (PVA) matrix to form PVA/CG mixed‐matrix membranes (MMMs). The membranes demonstrated excellent mechanical properties and thermal stability. The improved hydrophilicity and formed crosslinking structure led to moderate swelling. The membrane crystallinity decreased and the free volume was promoted with increasing CG loading amount. The pervaporation (PV) separation performance for ethanol dehydration indicated that both permeation flux and separation factor were enhanced simultaneously at the optimum CG loading. Subsequently, the permeation flux continued to increase while the separation factor declined at higher CG loadings.

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Carboxyl-functionalized graphene oxide composites as SPR biosensors with enhanced sensitivity for immunoaffinity detection

Cited by 139 publications

References 49 publications

High-Sensitivity Detection of the Lung Cancer Biomarker CYFRA21-1 in Serum Samples Using a Carboxyl-MoS2 Functional Film for SPR-Based Immunosensors

High-Sensitivity Detection of the Lung Cancer Biomarker CYFRA21-1 in Serum Samples Using a Carboxyl-MoS2 Functional Film for SPR-Based Immunosensors

Functionalized Graphene-Based Materials as Innovative Adsorbents of Organic Pollutants: A Concise Overview

Poly(vinyl alcohol)/Carboxyl Graphene Membranes for Ethanol Dehydration by Pervaporation

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