Carboxyhaemoglobin. spectrophotometric determination tested and calibrated using a new reference method for measuring carbon monoxide in blood

Dijkhuizen, P.; Buursma, Anneke; Gerding, Albert; Kampen, E. van; Zijlstra, W. G.

doi:10.1016/0009-8981(77)90268-6

Cited by 17 publications

(6 citation statements)

References 20 publications

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“…The constants of Eq. 6 have been determined by calibration against a titrimetric method for HbCO in blood (16).…”

Section: Conventional Methodsmentioning

confidence: 99%

A Multi-Wavelength Spectrophotometric Method for the Simultaneous Determination of Five Haemoglobin Derivatives

Zwart¹,

Buursma²,

Kampen³

et al. 1981

CCLM

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A method is described by which the concentration of deoxyhaemoglobin, oxyhaemoglobin, carboxyhaemoglobin, haemigjobin and sulphaemoglobin in a human blood sample is determined by passing the haemolysate without air contact through a coarse filter and subsequently measuring the absorbance at = 500, 569, 577, 620 and 760 nm. The euouing set of equations is solved by matrix calculation with the aid of a simple computer program. The method has been tested by comparing it with conventional methods for the determination of the various haemoglobin derivatives separately. Eine Viel-Wellenlängen Methode für die simultane spektrophotometrische Bestimmung von fünf HämoglobinderivatenZusammenfassung: Eine Methode, mit der die Konzentration von Desoxyhämoglobin, Oxyhämoglobin, Carboxyhämoglobin, Hämiglobin und Sulfhämogjobin in einer Blutprobe vom Menschen bestimmt werden kann, wird beschrieben. Das Hämolysat durchläuft ohne Luftkontakt ein grobes Filter; die Absorption bei = 500, 569, 577, 620 und 760 nm wird gemessen. Der sich ergebende Satz von Gleichungen wird durch Matrizen-Berechnung mit Hilfe eines einfachen Rechnerprogramms gelöst. Die Methode wurde durch Vergleich mit konventionellen Methoden für die separate Bestimmung der verschiedenen Hämoglobinderivate geprüft.

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“…The constants of Eq. 6 have been determined by calibration against a titrimetric method for HbCO in blood (16).…”

Section: Conventional Methodsmentioning

confidence: 99%

A Multi-Wavelength Spectrophotometric Method for the Simultaneous Determination of Five Haemoglobin Derivatives

Zwart¹,

Buursma²,

Kampen³

et al. 1981

CCLM

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“…Two samples of 400 ml of blood in a 2-liter round-bottom flask was purged either with CO (Tatragas Messer-Slovakia, purity 99.9 %) with the flow rate 5 l/min during simultaneous shaking (100 min -1 ) for 15 min = 100 % COHb or with medicinal oxygen (Tatragas Messer-Slovakia, purity 99.9 %) under the same conditions = 0 % COHb. The COHb content in blood was determined according to Dijkhuizen et al (1977) by UV-VIS Diode array spectrophotometer Multispec-1501 (Shimadzu, Japan). To 2000 μl of distilled water (Millipore-Simplicity, France) was added 2 μl of blood and vigorously mixed in the 1 cm quartz cell.…”

Section: In Vitro Experiments Removal Of Co From Cohb In Human Blood Umentioning

confidence: 99%

Is Ionized Oxygen Negatively or Positively Charged More Effective for Carboxyhemoglobin Reduction Compare to Medical Oxygen at Atmospheric Pressure?

Perečinský

Kron²,

Engler³

et al. 2015

Physiol Res

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Carbon monoxide (CO) reversibly binds to hemoglobin forming carboxyhemoglobin (COHb). CO competes with O2 for binding place in hemoglobin leading to tissue hypoxia. Already 30 % saturation of COHb can be deadly. Medical oxygen at atmospheric pressure as a therapy is not enough effective. Therefore hyperbaric oxygen O2 inhalation is recommended. There was a question if partially ionized oxygen can be a better treatment at atmospheric pressure. In present study we evaluated effect of partially ionized oxygen produced by device Oxygen Ion 3000 by Dr. Engler in elimination of COHb in vitro experiments and in smokers. Diluted blood with different content of CO was purged with 5 l/min of either medicinal oxygen O2, negatively ionized O2 or positively ionized O2 for 15 min, then the COHb content was checked. In vivo study, 15 smokers inhaled of either medicinal oxygen O2 or negatively ionized O2, than we compared CO levels in expired air before and after inhalation. In both studies we found the highest elimination of CO when we used negatively ionized O2. These results confirmed the benefit of short inhalation of negatively ionized O2, in frame of Ionized Oxygen Therapy (IO2Th/Engler) which could be used in smokers for decreasing of COHb in blood.

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“…A critical discussion of these methods has been performed in order to propose the best method for the determination of COHb in postmortem blood that could be utilized in forensic toxicology laboratories where such analyses are limited in number (less than 20 per year). Validation of the discussed methods has been based on previously reported comparative studies (Dijkhuizen et al 1977; Baretta et al 1978; Dennis and Valeri 1980; Katsumata et al 1982; Zwart et al 1984; Vreman, Kwong, and Stevenson 1984; Goldbaum, Chace, and Lappa 1986; Costantino, Park, and Caplan 1986; Vreman, Stevenson, and Zwart 1987; Fechner and Gee 1989; Mahoney et al 1993; Van Dam, Pharm, and Daenes 1994; Levine et al 1996; Canfield et al 1999; Oritani et al 2000). Criteria for the evaluation have been considered simplicity, accuracy and precision of results, as well as time and cost of analysis.…”

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confidence: 99%

Evaluation of the Methods Used for Carboxyhemoglobin Analysis in Postmortem Blood

Boumba

Vougiouklakis

2005

Int J Toxicol

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Numerous methods have been described in the literature for the determination of carboxyhemoglobin (COHb) in whole blood. The most popular and widely used have been (1) the spectrophotometric methods, which could be performed either by using a conventional spectrophotometer or by using specialized automated instruments known as CO-oximeters; (2) the gas chromatographic methods, with variable detection systems, which have been considered as the reference methods for every carbon monoxide analysis. The authors have critically reviewed previously reported comparative studies on these methods, considering statistical and analytical matters, in order to propose the best method for the determination of COHb in postmortem blood, that could be utilized in forensic toxicology laboratories where such analyses are limited in number (less than 20 per year). Criteria for evaluation have been accuracy, reliability, simplicity, time, and cost. The authors' concluding statement has been that the manual spectrophotometric method could be the method of choice for COHb determination in postmortem blood samples. It is simple, rapid, and reliable and fulfills the forensically acceptable accuracy. It is performed by the use of a conventional spectrophotometer, which is considered a basic instrument in every analytical laboratory.

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Carboxyhaemoglobin. spectrophotometric determination tested and calibrated using a new reference method for measuring carbon monoxide in blood

Cited by 17 publications

References 20 publications

A Multi-Wavelength Spectrophotometric Method for the Simultaneous Determination of Five Haemoglobin Derivatives

A Multi-Wavelength Spectrophotometric Method for the Simultaneous Determination of Five Haemoglobin Derivatives

Is Ionized Oxygen Negatively or Positively Charged More Effective for Carboxyhemoglobin Reduction Compare to Medical Oxygen at Atmospheric Pressure?

Evaluation of the Methods Used for Carboxyhemoglobin Analysis in Postmortem Blood

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