We determined the millimolar absorptivities of the four clinically relevant derivatives of fetal and adult human hemoglobin in the visible and near-infrared spectral range (450-1000 nm). As expected, spectral absorption curves of similar shape were found, but the small differences between fetal and adult hemoglobin absorptivity were important enough that they should be taken into account in multicomponent analysis of hemoglobin derivatives. Common pulse oximeters, however, involving light of 660 and 940 nm, are so insensitive to the presence of fetal hemoglobin that they can be used safely in neonates. The error in pulse oximetry caused by the presence of carboxyhemoglobin is insubstantial, but methemoglobin gives either an understimation or an overestimation at high or low oxygen saturation, respectively, the turning point being near 70% saturation.
A method is described by which the concentration of deoxyhaemoglobin, oxyhaemoglobin, carboxyhaemoglobin, haemigjobin and sulphaemoglobin in a human blood sample is determined by passing the haemolysate without air contact through a coarse filter and subsequently measuring the absorbance at = 500, 569, 577, 620 and 760 nm. The euouing set of equations is solved by matrix calculation with the aid of a simple computer program. The method has been tested by comparing it with conventional methods for the determination of the various haemoglobin derivatives separately.
Eine Viel-Wellenlängen Methode für die simultane spektrophotometrische Bestimmung von fünf HämoglobinderivatenZusammenfassung: Eine Methode, mit der die Konzentration von Desoxyhämoglobin, Oxyhämoglobin, Carboxyhämoglobin, Hämiglobin und Sulfhämogjobin in einer Blutprobe vom Menschen bestimmt werden kann, wird beschrieben. Das Hämolysat durchläuft ohne Luftkontakt ein grobes Filter; die Absorption bei = 500, 569, 577, 620 und 760 nm wird gemessen. Der sich ergebende Satz von Gleichungen wird durch Matrizen-Berechnung mit Hilfe eines einfachen Rechnerprogramms gelöst. Die Methode wurde durch Vergleich mit konventionellen Methoden für die separate Bestimmung der verschiedenen Hämoglobinderivate geprüft.
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